@article {2114, title = {Activity of Pegagan Embun (Hydrocotyle sibthorpioides Lam.) Extracts in Capsule Dosage Form to IgM and IgG Levels in Humans}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {October 2023}, pages = {756-760}, type = {Original Article}, chapter = {756}, abstract = {

Objective: Traditionally, Pegagan embun (Hydrocotyle sibthorpiodes Lam.) has been used to enhance the immune system. Previous studies confirmed that Hydrocotyle sibthorpiodes Lam. enhanced antibody titers and was practically non-toxic by animal tests. This study aimed to determine the effect of Hydrocotyle sibthorpioides Lam. extract on IgM and IgG levels in humans. Methods: The participants involved 20 healthy volunteers aged 20-25 years were divided into two groups with a placebo as a comparison. The capsules containing Hydrocotyle sibthorpiodes Lam extract were in the treatment group at a dose of 67 mg and the placebo group was given capsules containing lactose orally for three days. IgM and IgG levels were measured before and after consuming the capsules. The ELISA method tested IgM and IgG levels and detected them using specific antibodies that form complexes with enzymes and substrates. Results: The results showed the average baseline IgM level of 1.480 mg/mL and after consumption of 1.405 mg/mL for placebo. For Hydrocotyle sibthorpiodes Lam. extract capsules, the baseline was 1.492 mg/mL and 1.854 mg/mL after consumption. For IgG, before given 8.941 mg/mL and after given 8.710 mg/mL for placebo, while before given Hydrocotyle sibthorpiodes Lam. extract capsules 8.946 mg/mL and after given Hydrocotyle sibthorpiodes Lam. extract capsules was 10.234 mg/mL. Statistical results showed a significant increase in IgM and IgG levels after consuming Hydrocotyle sibthorpiodes Lam. extract capsules for three days (p\<0.05). Conclusion: It can be concluded that 67 mg of Hydrocotyle sibthorpiodes Lam. extract capsules can increase IgM and IgG levels in humans.

}, keywords = {ELISA, Extract, Hydrocotyle sibthorpioides Lam, IgG, IgM, Volunteers.}, doi = {10.5530/pj.2023.15.148}, author = {Afriwardi Afriwardi and Adinny Julmiza and Salman Umar and Yufri Aldi} } @article {849, title = {ELISA Test on Cordia myxa L. Leaf Extract for alpha-Glucosidase Inhibitor}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {February 2019}, pages = {358-361}, type = {Original Article}, chapter = {358}, abstract = {

Aimed: Determine the potential of Cordia myxa L. leaf on inhibited α-glucosidase. Material: ELISA Kit, Ethanol 96\%, Colomn Chromatography, n-hexane, ethyl acetate, Glocobay{\textregistered}.\  Method: Sample from Cordia myxa L. leaf extracted by ethanol 96\% then evaporated to get the sticky extract. The sticky extract of Cordia myxa L. leaf fractionated by column chromatography with n-hexane, n-hexane: ethyl acetate (90:10; 80:20; 75:25; 70:30; 65:35; 60:40; 55:45; 50:50) Assay: The fractions assayed by ELISA (Enzyme-Linked Immunosorbent Assay) with acarbose (Glucobay {\textregistered}) as the comparator. Result: The results showed that the n-hexane fraction is the highest potency on inhibited α-glucosidase with the noncompetitive mechanism. The IC50 of n-hexane fraction is 0.53 ppm been while the acarbose is 6.85 ppm. Conclusion: The n-hexane fraction of Cordia myxa L. leaf has the highest potency to use for possible decrease blood glucose level.

}, keywords = {Acarbose, Cordia myxa L., ELISA, IC50, α-glucosidase}, doi = {10.5530/pj.2019.11.54}, author = {Ahmad Najib and Aktsar Roskiana Ahmad and Virsa Handayani} }