@article {810, title = {Effect of Gamma Irradiation on Some Pharmacological Properties and Microbial Activities of Melinjo (Gnetum gnemon Linn.) Seeds}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {January 2019}, pages = {177-182}, type = {Original Article}, chapter = {177}, abstract = {

Background: Ionizing radiation, such as gamma irradiation, serves as a useful approach to inhibit spore germination and to control pathogens in postharvest seeds. Recently, its application on phytochemical sources and its influence on antioxidant activity of various phytochemical compounds has become an interesting topic to be explored. Objective: The objectives of this study were to determine the effect of gamma irradiation as sterilization method on the resveratrol content and its antioxidant, HMG-CoA reductase inhibitory and dipeptidyl peptidase-4 (DPP-4) inhibitory activities of Melinjo (Gnetum gnemon) seeds. Methods: In this research, melinjo seeds were irradiated by 0.0; 2.5; 5.0; 7.5; and 10.0 kGy with gamma irradiation and then extracted with ethanol. The extracts were tested for resveratrol content with HPLC, antioxidant activities by DPPH assay, HMG-CoA inhibitory activity using HMG-CoA reductase assay kit and DPP-4 inhibitory activity using DPP-4 Inhibitor Screening Assay Kit. Gamma irradiation has effect on resveratrol content, antioxidant activity, HMG-CoA reductase inhibition and DPP-4 inhibitory activity. Results: From the research, the highest value of resveratrol content is 0.18{\textpm}0.004 mg/g seeds powder found in 5.0 kGy gamma irradiation treatment with IC50 94.64{\textpm}0.236 μg/mL, while the highest HMG-CoA reductase inhibition is shown in 2.5 kGy irradiation dose. Melinjo seeds irradiated by 2.5 kGy gamma irradiation also shown a significant increase of DPP-4 inhibition activity. Conclusion: This study suggests that 2.5-5 kGy radiation is the effective gamma irradiation dose to improve the quality of melinjo seeds.

}, keywords = {Antioxidant, Dipeptidyl peptidase-4, Gamma irradiation, Gnetum gnemon, HMG-CoA reductase, Resveratrol}, doi = {10.5530/pj.2019.1.29}, author = {Rezi Riadhi Syahdi and Aditya Sindu Sakti and Agung Kristiyanto and Riky Redmawati and Abdul Mun{\textquoteright}im} } @article {766, title = {HMG-CoA Reductase Inhibitory Activity of Garcinia latissima Miq. Mesocarp Water Extract for Herbal Tea}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {November 2018}, pages = {s141-s146}, type = {Original Article}, chapter = {s141}, abstract = {

Context: High cholesterol in the blood is a risk factor for atherosclerosis that causes various diseases. The main pharmacologic intervention to reduce cholesterol levels is inhibiting the HMG-CoA reductase enzyme. One of the genera of Garcinia, Garcinia dulcis, has potential as an anti-cholesterol. Based on chemotaxonomy, Garcinia latissima Miq. is also estimated to have a potency as anti-cholesterol. Aims: This study aims to test the inhibition of HMG-CoA reductase water extract activity of G. latissima fruit flesh with different duration of infusions. Materials and Methods: Garcinia latissima Miq. mesocarp was extracted using infusion method with different duration of infusions. Each of extracts was tested the inhibitory activity of HMG-CoA reductase as well as the determination of total flavonoid and total phenol content. In addition, the simplicia of the mesocarp of G.latissima Miq. will be made as a herbal tea and a hedonic test is performed to find out the degree of liking for the tea. Result: The test results showed the inhibitory activity of 100 ppm G. latissima Miq. mesocarp water extract with infusion for 5, 10 and 15 min respectively 11.32; 29.02; 13.03\%. The 10 min extract with the largest enzyme inhibition had total flavonoids content of 31.24 mg QE / gram extract and total phenol content of 4.64 mg GAE/ gram extract. The result of the hedonic test for the colour, aroma, flavour of herbal tea formula A respectively 30; 30; 20\% and formula B respectively for 40; 33.3; 50\%. Conclusion: The water extract of G.latissima Miq mesocarp has a low potency in HMG-CoA reductase inhibitory activities.

}, keywords = {Anti Cholesterol, Garcinia Latissima, Herbal Tea, HMG-CoA reductase, Mesocarp}, doi = {10.5530/pj.2018.6s.26}, author = {Herra Williany Monalissa and Berna Elya and Nuraini Puspitasari} }