@article {2246, title = {Boletus griseipurpureus Corner: Antibacterial, Antioxidant Properties and Phytochemical Compositions}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {April 2024}, pages = {296-301}, type = {Original Article}, chapter = {296}, abstract = {

This study aims to investigate the properties of B. griseipurpureus Corner concerning its efficacy against various bacterial strains and its antioxidant capacity, along with its phytochemical composition. The antibacterial activity was evaluated using the agar well diffusion method, revealing pronounced efficacy against gram-negative bacteria, particularly noteworthy against E. coli ESBL182, known for its antibiotic resistance. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of B. griseipurpureus Corner extract against E. coli ESBL182 were determined to be 75 and 300 mg/ml, respectively. The extract exhibited bacteriostatic activity by inhibiting cell growth, leading to a reduction in optical density at 600 nm. Additionally, examination of the supernatant from untreated and treated E. coli ESBL182 cells with B. griseipurpureus Corner extract indicated absorbance values of 0.088 and 0.248 at 260 nm, and 0.045 and 0.286 at 280 nm, respectively. Further investigation utilizing a scanning electron microscope revealed alterations in the morphology of treated cells, which displayed elongation and fragmentation, in contrast to untreated cells. The DPPH assay indicated that the IC50 of B. griseipurpureus Corner extract was 31.22 mg/ml. The IC50 value obtained from the ABTS assay was 47.31 mg/ml. Additionally, the FRAP assay revealed that the concentration of ascorbic acid equivalent in B. griseipurpureus Corner extract was 1.06 mg/g crude extract. Phytochemical analysis, conducted using a spectrophotometer at wavelengths of 750 nm and 510 nm, respectively, indicated phenolic and flavonoid contents of 0.22 mg gallic acid/g fresh weight and 3.23 mg quercetin/g fresh weight in the extract.

}, keywords = {Antibacterial activity, Antioxidant property, Boletus griseipurpureus Corner, Flavonoid compound, Phenolic compound, Phytochemical compositions}, doi = {10.5530/pj.2024.16.44}, author = {On-Anong Somsap and Anussara Kamnate and Amornrat Angajchariya} } @article {1152, title = {Development and Validation of Stability Indicating HPLC Method for Determination of Caffeic Acid, Vitexin and Rosmarinic Acid in Thunbergia laurifolia Leaf Extract}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {611-618}, type = {Research Article}, chapter = {611}, abstract = {

Thunbergia laurifolia has been a popular herb used in Thai traditional medicine for detoxification and as antipyretic. It contains rosmarinic acid (RA), caffeic acid (CA) and vitexin as major compounds. In order to control the herbal quality, the stability indicating high-performance liquid chromatography (HPLC) was developed and validated. The stability study of compounds in T. laurifolia leaf extract was investigated. The chromatographic separation was performed using a reversed-phase C18 column and mobile phase consisted of 0.5\% acetic acid and methanol using a gradient elution with 1.0 mL/min flow rate. The detection wavelength was set at 330 nm. The method was validated for its linearity, precision, accuracy, limit of detection and limit of quantitation. Forced degradation of three compounds in extract showed that they were stable in oxidative condition, but highly labile under alkaline hydrolytic conditions. All three compounds in T. laurifolia leaf extract were stable at room temperature at least 3 months while a remarkable decrease of RA, vitexin and CA in the extract were found in accelerated condition. This finding could be applied for predicting the storage recommendation and expiry of T. laurifolia extract and its related pharmaceutical products.

}, keywords = {Force degradation, Phenolic compound, Phytochemical screening, Stabilityindicating method}, doi = {10.5530/pj.2020.12.91 }, author = {Nanthakarn Woottisin and Sumet Kongkiatpaiboon and Sophida Sukprasert and Korbtham Sathirakul} } @article {743, title = {Antioxidant Activities of Ethanolic and Aqueous Extracts of Asparagus racemosus Roots}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {1129-1132}, type = {Original Article}, chapter = {1129}, abstract = {

Background: Asparagus racemosus (AR) is commonly known as shatavari, satawar or satmuli in India and in Thailand it is call sam-sib or rak-sam-sib. The dried root of AR is used in Ayurveda as an antiulcerous and antiinflammatory and has medicinal/pharmacological value. Objective: To investigate the antioxidant activities of Asparagus racemosus root extracts via total phenolic and total flavonoid contents of ethanolic and aqueous extracts. Methods: Antioxidant capacity measurements were carried out by DPPH, ABTS and FRAP methods. Total phenolic and flavonoid contents were determined by the Folin-Ciocalteu method and the aluminum chloride colorimetric method, respectively. Results: The ethanolic extract possessed higher antioxidant capacities than the aqueous extract in the three antioxidant assays (p\<0.05).These results have shown high phenolic and flavonoid contents. The ethanolic extract of AR root possessed higher amounts of phenolic and flavonoid contents than the aqueous extract. Conclusion: The antioxidant capacity of the ethanolic extract was higher than that in the aqueous extract.

}, keywords = {antioxidant activity, Asparagus racemosus, Flavonoid, Phenolic compound}, doi = {10.5530/pj.2018.6.192}, author = {Ladachart Taepongsorat and Surapong Rattana} }