@article {997, title = {Effect of Beta Glucosidase Inhibitor from Lichen Extract in Microcrystalline Cellulose Preparation from Water Hyacinth (Eichhornia crassipes)}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2019}, pages = {1199-1203}, type = {Original Article}, chapter = {1199}, abstract = {

Objective: Microcrystalline cellulose (MCC) is an excipient commonly used in the manufacturing of pharmaceutical preparations, especially tablet. MCC has been successfully made from water hyacinth (Eichhornia crassipes) through enzymatic hydrolysis process. This study aimed to find the effect of β-glucosidase inhibitor from lichen extract to the yield of MCC, the optimum conditions of enzymatic hydrolysis including pH and reaction time and characteristics of MCC obtained compared to reference, Avicel PH-101. Method: The study was began with extraction of cellulose enzyme and followed by determination of optimum beta-glucosidase inhibitor concentration. Then, this conditions were used for enzymatic hydrolysis of α-cellulose to MCC, followed by identification and characterization of MCC obtained and compared with Avicel PH- 101. Results: The optimum concentration of inhibitor was 120 ppm. A little higher yield of MCC was obtained when inhibitor extract is used in hydrolysis alfa-cellulose to MCC. The identity of MCC obtained was similar to infrared spectrum of reference. Other characteristics of MCC obtained were powder in the form of a slightly coarse, odorless and tasteless and slightly yellowish than reference. Conclusion: Addition of extract of Beta glucosidase inhibitor did not improve the MCC yield significantly, but identity and characteristics of MCC obtained showed similarities to the microcrystalline cellulose reference (Avicel PH = 101).

}, keywords = {Enzymatic hydrolysis, Lichen extract, Microcrystalline cellulose, Water hyacinthm, β-glucosidase inhibitor}, doi = {10.5530/pj.2019.11.186}, author = {Citra Bonnita Putri and Sutriyo and Herman Suryadi} } @article {716, title = {Isolation of Cellulolytic Fungi and Utilization of Its Cellulolytic Activity for Microcrystalline Cellulose Preparation from Water Hyacinth (Eichhornia crassipes)}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {1082-1088}, type = {Original Article}, chapter = {1082}, abstract = {

Introduction: Microcrystalline cellulose is a cellulase derivative which usually used as a pharmaceutical excipient in the manufacturing of direct compression tablet. High concentration of cellulose is found in water hyacinth (about 60\%) therefore it might be used in the manufacturing of microcrystalline cellulose. This study was aimed to obtain the best cellulolytic fungi, the best optimal conditions of enzymatic hydrolysis and comparing microcrystalline cellulose characteristics obtained from water hyacinth with microcrystalline cellulose standard, Avicel pH 101. Method: This study began with isolation of cellulolytic fungi, and then enzymatic hydrolysis of \α-cellulose was done with cellulase enzymes extracted from cellulolytic fungi with various pH, temperature, duration and enzyme concentration. Microcrystalline obtained was characterized by XRD (X-Ray Diffraction) and then the result was compared with Avicel pH 101. Results: The results showed the best cellulolytic isolate was isolate 2 and its optimal hydrolysis conditions at pH 5, 30$^{0}$C, for 1 h with 5 mL (5\% v/v) enzyme. Based on the pattern of diffraction there was a similarity between microcrystalline cellulose of enzymatic hydrolysis result compared with Avicel pH 101.

}, keywords = {Cellulase, Cellulolytic fungi, Cellulose, Enzymatic hydrolysis, Microcrystalline cellulose, Water hyacinth}, doi = {xx10.5530/pj.2018.6.183}, author = {Mitayani Wahyu Murti and Monica Angeline Sudarsono and Herman Suryadi} }