@article {2341, title = {In Silico Gene Transcription of 4-Hydroxycinnamic Acid from Broccoli Fruit (Brassica oleracea var. italica) with Estrogen Receptor Beta Protein}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {August 2024}, pages = {791-796}, type = {Original Article}, chapter = {791}, abstract = {

This study aims to explore the relationship between 4-Hydroxycinnamic Acid, a compound found in broccoli fruit (Brassica oleracea var. italica), and Estrogen Receptor Beta Protein through in silico analysis. The research was conducted using Pymol, MOE 2015, Discovery Studio 2016, and Lepinski Rule software. These tools were employed to model the structure of both the compound and protein, as well as to evaluate their interaction. The results revealed a significant interaction with a Binding Affinity of -6.4182. Moreover, the Root Mean Square Deviation (RMSD) measurements yielded a value of 3.8907, indicating the degree of agreement between the compound and protein structures. Analysis using the Lepinski Rule disclosed the characteristics of the compound 4-Hydroxycinnamic Acid, including a mass of 164, two hydrogen bond donors, three hydrogen bond acceptors, a log P value of 1.490, and a molar reactivity of 44.776. These findings provide valuable insights into the potential of 4-Hydroxycinnamic Acid in interacting with Estrogen Receptor Beta Protein, which could serve as a foundation for further research on the health benefits and therapeutic applications of this compound, contributing to the goal of good health and well-being.

}, keywords = {Brassica oleracea var. italica, good health and well-being., in silico, molecular interaction}, doi = {10.5530/pj.2024.16.131}, author = {Rahadian Zainul and Dheo Shalsabilla Novel and Herland Satriawan and Khang Wen Goh and Vikash Jakhmola and Maksim Rebezov and Sri Suwarni and Ria Septiana and Arif Nur Muhammad Ansori and Mochammad Aqilah Herdiansyah and Anni Faridah} } @article {2344, title = {Molecular Insights into Spirulina platensis Compounds Targeting TNFa and P21 in Delaying Cellular Senescence Mechanisms: An In Silico Approach}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {August 2024}, pages = {809-815}, type = {Original Article}, chapter = {809}, abstract = {

Spirulina platensis with bioactive compounds such as Phycocyanin, β-carotene, Genistein and α-glucan, have been shown to have anti-inflammatory properties. Our present study investigation utilizes an in silico methodology to examine the molecular interactions between Spirulina platensis chemicals compound with TNFa and p21. Utilizing computational techniques molecular docking, our goal is to clarify the possible pathways by which Spirulina platensis chemicals could affect these important regulators and postpone cellular senescence. Our research may shed important light on the creation of Spirulina platensis-based therapies for ageing and age-related illnesses. Our results imply that Spirulina platensis may contribute to overall cellular health and the mitigation of cellular senescence. Phycocyanin has the most negative ΔG value is -15.0 kcal/mol. Genistein has the lowest Ki value, namely 7.299 μM. The ΔG and Ki values of Genistein were lower than Quercetin. The potential chemical interactions between substances generated from Spirulina platensis and senescence pathways, including those involving TNFa and p21, are highly intriguing for the development of innovative therapeutic approaches targeted at ameliorating cellular senescence dysfunction associated with aging.

}, keywords = {in silico, p21 expression, Spirulina platensis, TNFa-induced cellular senescence}, doi = {10.5530/pj.2024.16.134}, author = {Tiwuk Susantiningsih and Fadilah Fadilah and Ani Retno Prijanti and Novi Silvia Hardiany} } @article {1803, title = {Azasterol Inhibition and Pharmacokinetic Effects on Thymidylate Synthase-Dihydrofolate Reductase from T. gondii: In Silico Study}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {June 2022}, pages = {571-575}, type = {Research Article}, chapter = {571}, abstract = {

Toxoplasmosis is a disease that causes health problems and can be found worldwide with a percentage of more than 60\%, especially in developing countries such as Indonesia. Pyrimethamine-resistant strains of T. gondii have been found, and it may contribute to reducing therapeutic failure in the future. Azasterol is a synthetic analog of solacongestidine, which can potentially be used as a new anti-toxoplasma drug. Resistance to the anti-toxoplasma drug, Pyrimethamine, makes Azasterol a very profitable discovery as a new anti-toxoplasma drug. This study aimed to determine the inhibitory and pharmacokinetic effects of Azasterol compounds on the development of T. gondii based on in silico studies. This oneshot experimental study analyzed the predicted inhibitory effect of Azasterol on Thymidylate synthasedihydrofolate reductase (TS-DHFR) from T. gondii to observe the pharmacokinetic prediction and toxicity test of the Azasterol compound. Besides, this one-shot experimental study utilized the in silico method. According to the results of molecular docking, Azasterol had an interaction with the TS-DHFR protein in the same binding area as the Pyrimethamine {\textendash} TS-DHFR and Sulfadiazine {\textendash} TS-DHFR complexes. Azasterol binding energy was higher than that of Pyrimethamine and Sulfadiazine. Azasterol had a good pharmacokinetic effect and had minimal toxic effects on the body.

}, keywords = {Azasterol, in silico, Toxoplasmosis, TS-DHFR.}, doi = {10.5530/pj.2022.14.73}, author = {Alan Dharmasaputra and Risma and Annisa Ullya Rasyida} } @article {1841, title = {Bioactive Compounds from Purslane (Portulaca oleracea L.) and Star Anise (Illicium verum Hook) as SARS-CoV-2 Antiviral Agent via Dual Inhibitor Mechanism: In Silico Approach}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {352-357}, type = {Original Article }, chapter = {352}, abstract = {

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the COVID-19 pandemic that infects humans and attacks the body{\textquoteright}s immune system. The purpose of the study was to identify the potential of bioactive compounds in purslane (Portulaca oleracea L.) and star anise (Illicium verum Hook) via a dual inhibitor mechanism against SARS-CoV-2 proteases with an in silico approach. The samples were obtained from PubChem and RSCB PDB. Antivirus probability prediction was performed on PASS Online. Virtual screening was performed with PyRx via molecular docking. Visualization was used by PyMol and Discovery Studio. Compounds with the best antiviral potential are indicated by the low binding affinity value to the target proteins, namely SARS-CoV-2 TMPRSS2 and PLpro. The results showed that purslane luteolin has the best antiviral potential. However, further studies are required to validate this computational prediction.

}, keywords = {Antiviral agent, Illicium verum Hook, in silico, Portulaca oleracea L., SARS-CoV-2}, doi = {10.5530/pj.2022.14.106}, author = {Nur Sofiatul Aini and Viol Dhea Kharisma and Muhammad Hermawan Widyananda and Ahmad Affan Ali Murtadlo and Rasyadan Taufiq Probojati and Dora Dayu Rahma Turista and Muhammad Badrut Tamam and Vikash Jakhmola and Dony Novaliendry and Riso Sari Mandeli and Budhi Oktavia and Muhammad Thoriq Albari and Saddam Al Aziz and Muhammad Raffi Ghifari and Okta Suryani and Putri Azhari and Muhammad Arya Ghifari and Devi Purnamasari and Agariadne Dwinggo Samala and Mirella Fonda Maahury and ANM Ansori and Rahadian Zainul} } @article {1813, title = {In silico Analysis of the Polyphenolic Metabolites of Zea mays L. "Purple Corn" on HMG-CoA Reductase}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {June 2022}, pages = {549-558}, type = {Original Article}, chapter = {549}, abstract = {

This research aims to identify the polyphenolic metabolites, reported in ears and grains of Zea mays L. "purple corn" according to the current literature, with more significant interaction on HMG-CoA reductase, through in silico assays. Using the keyword combination {\textquotedblleft}Zea mays L{\textquotedblright} AND {\textquotedblleft}polyphenols{\textquotedblright}, a search was made in Google Scholar, PubMed, ScienceDirect and Scopus databases, identifying 22 polyphenolic compounds. Polyphenolic ligands and control molecules were prepared with the OpenBabel program and parameterized with AutoDock Tools. In addition, the crystallized structure of HMG-CoA reductase (1DQA) was downloaded from the Protein Data Bank database, then prepared in PyMOL and parameterized with AutoDock Tools. Molecular docking was performed in AutoDock Vina with a 100-time repetition for each ligand-target interaction. The results show that the hydrogen bonds with amino acids of importance in HMG-CoA reductase are ASN 658, ARG 590, and GLU 559. Protocatechuic acid, caffeic acid, vanillic acid, ferulic acid, p-coumaric acid, and 4-hydroxybenzoic acid presented lower affinity energy (ΔG{\textdegree}). The physicochemical and pharmacokinetic properties of the molecules with the best pharmacodynamic interaction were analyzed with the SwissADME and pkCSM servers, showing that protocatechuic, caffeic, vanillic, ferulic, p-coumaric and 4-hydroxybenzoic acids have the best physicochemical and pharmacokinetic profile. Therefore, this study gives us a clearer idea of the action of polyphenols on HMG-CoA reductase, which will allow obtaining new drug candidates for the treatment of hypercholesterolemia.

}, keywords = {Flavonoids, HMG-CoA reductase., in silico, Polyphenols, Zea mays L.}, doi = {10.5530/pj.2022.14.70}, author = {Horna-Rodriguez Alexsandra M and L{\'o}pez-Gamboa July A and Silva-Correa Carmen R and Sag{\'a}stegui-Guarniz William Antonio and Gamarra-S{\'a}nchez C{\'e}sar D and Villarreal-La Torre V{\'\i}ctor E} } @article {1873, title = {In Silico Screening of Bioactive Compounds from Garcinia mangostana L. Against SARS-CoV-2 via Tetra Inhibitors}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {October 2022}, pages = {575-579}, type = {Research Article}, chapter = {575}, abstract = {

The global COVID-19 pandemic caused by SARS-CoV-2 has been the resulted of massive human deaths since early 2020. The purpose of this study was to determine the potential of mangosteen (Garcinia mangostana L.) as an inhibitor of RBD spike, helicase, Mpro, and RdRp activity of SARS-CoV-2 with an in silico approach. The samples were obtained from PubChem and RCSB PDB. Analysis of the similarity of the drug was carried out with the Swiss ADME on the basis of Lipinski rule of five. Prediction of antivirus probabilities was carried out using PASS Online. Molecular screening was performed using PyRx through molecular docking. Discovery Studio was used for visualization. The bioactive compounds with the highest antiviral potential were indicated with the lowest binding affinity to the targeted proteins RBD spike, helicase, Mpro, and RdRp of SARS-CoV-2. The results indicated that mangiferin has the greatest potential as a potential antiviral. However, more research is required to validate the results of these computational predictions.

}, keywords = {Antiviral agent, Garcinia mangostana L., in silico, SARS-CoV-2}, doi = {10.5530/pj.2022.14.138}, author = {Nur Sofiatul Aini and Viol Dhea Kharisma and Muhammad Hermawan Widyananda and Ahmad Affan Ali Murtadlo and Rasyadan Taufiq Probojati and Dora Dayu Rahma Turista and Muhammad Badrut Tamam and Vikash Jakhmola and Elsa Yuniarti and Saddam Al Aziz and Muhammad Raffi Ghifari and Muhammad Thoriq Albari and Riso Sari Mandeli and Muhammad Arya Ghifari and Devi Purnamasari and Budhi Oktavia and Amalia Putri Lubis and Fajriah Azra and Fadhilah Fitri and ANM Ansori and Maksim Rebezov and Rahadian Zainul} } @article {1832, title = {In Silico Screening of Bioactive Compounds from Syzygium cumini L. and Moringa oleifera L. Against SARS-CoV-2 via Tetra Inhibitors}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {267-272}, type = {Original Article }, chapter = {267}, abstract = {

The global pandemic of COVID-19 has caused disastrous consequences for both humans and the economy. The purpose of this study was to determine the potential of juwet (Syzygium cumini L.) and moringa (Moringa oleifera L.) as inhibitors of RBD spike, helicase, Mpro, and RdRp activity of SARS-CoV-2 with an in-silico approach. Samples were obtained from PubChem and RSCB PDB databases. The drug similarity analysis was determined using Swiss ADME and the Lipinski rule of five. Prediction of antivirus probabilities is carried out with PASS Online. Molecular screening is performed by molecular docking using PyRx. Visualization was used using PyMol and Discovery Studio. The bioactive compounds with the best antiviral potential had the lowest affinity bonds to the target proteins against RBD spike, helicase, Mpro, and RdRp of SARS-CoV-2. Results show that ellagic acid from java plum and myricetin from moringa have the best potential as potential antivirals. However, more research is required to validate the results of these computational predictions.

}, keywords = {Antiviral agent, in silico, Moringa Oleifera L, SARS-CoV-2, Syzygium cumini L.}, doi = {10.5530/pj.2022.14.95}, author = {Nur Sofiatul Aini and Viol Dhea Kharisma and Muhammad Hermawan Widyananda and Ahmad Affan Ali Murtadlo and Rasyadan Taufiq Probojati and Dora Dayu Rahma Turista and Muhammad Badrut Tamam and Vikash Jakhmola and Devni Prima Sari and Muhammad Thoriq Albari and Devi Pernamasari and Muhammad Arya Ghifari and Muhammad Raffi Ghifari and Riso Sari Mandeli and Muhardi and Budhi Oktavia and Trisna Kumala Sari and Titi Sriwahyuni and Putri Azhari and Mirella Fonda Maahury and ANM Ansori and Rahadian Zainul} } @article {1773, title = {An In Silico Study of Examining Bioactive Compounds from Azadirachta indica Juss. (Neem) as Potential Death Receptor 5 Inductor in Hepatoma Cells}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {April 2022}, pages = {343-349}, type = {Research Article }, chapter = {343}, abstract = {

Hepatocellular carcinoma is a disease that occurs due to the uncontrolled growth of abnormal hepatocytes. While cancer cells will not die by itself, due to resistance to death receptors 5 (DR5)-mediated apoptosis. This study is aimed to investigate Azadirachta indica Juss. leaves compound, such as gedunin and nimbolide, in binding DR5 and stimulated the TNF-related apoptosis inducing ligand (TRAIL), native ligand binding to DR5, which has a role of pro-apoptotic by docking simulation. The ligand and protein preparations were done using Discovery Studio 2016 and Hex 8.0.0 for docking. Visualization was done using Discovery Studio 2016. The docking studies revealed that nimbolide has a lower binding energy with the DR5-TRAIL complex than gedunin. According to the findings, nimbolide is a more effective DR5-TRAIL binding inducer than gedunin and has a higher binding affinity for DR5-TRAIL. This interaction has the potential to significantly reduce DR5-TRAIL binding resistance. Nimbolide and gedunin can be considered as drugs that can sensitize TRAIL binding to DR5 and increase the activation of one of hepar cancers signaling apoptosis pathways.

}, keywords = {Apoptosis, Azadirachta indica Juss., Cancer, Death receptor 5, in silico}, doi = {10.5530/pj.2022.14.44}, author = {Ricadonna Raissa and Anna Safitri and Masruri Masruri and Ma Asuncion Guiang Beltran5 and Aulanni{\textquoteright}am Aulanni{\textquoteright}am} } @article {1912, title = {In Silico Study of the Potential of Endemic Sumatra Wild Turmeric Rhizomes (Curcuma Sumatrana: Zingiberaceae) As Anti-Cancer}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {December 2022}, pages = {806-812}, type = {Research Article }, chapter = {806}, abstract = {

Cancer is one of the diseases that is the highest cause of death in humans. Most human cancer cells are formed as a result of over-expression of anti-apoptotic proteins. Thus, the activation of these proteins can inhibit pro-apoptotic proteins, then apoptosis will be inhibited so that other apoptotic pathways need to be activated to prevent cancer cells from developing. Current cancer treatments, such as chemotherapy using synthetic compounds, have various side effects, so research on natural based therapies can be used as an alternative in cancer treatment. Curcuma sumatrana is one of the plants of the Zingiberaceae family which is an endemic plant from Sumatra which is found along the Bukit Barisan. The research was carried out in silico by analyzing the potential bioactivity of the compounds, testing the bioavailability, toxicity, and molecular docking of the bioactive compounds from the ethanol extract of the rhizome of C. sumatrana which had been previously identified through gas chromatography-mass spectroscopy (GCMS) analysis. The results obtained that the compound 9-Acetyl-S-octahydrophenanthrene and 3-Oxoandrosta- 1,4-dien-17.beta.-spiro-2{\textquoteright}-3{\textquoteright}-oxo-oxetanecontained in C. sumatrana has the potential to be developed as an anticancer where the compound has good bioavailability value and is not toxic and potentially can trigger apoptosis. However, the results of this study need to be analyzed further with an in vitro or in vivo approach.

}, keywords = {Anticancer, C. sumatrana, in silico}, doi = {10.5530/pj.2022.14.171}, author = {Aldi Tamara Rahman and Rafia and Aiken Jethro and Putra Santoso and Viol Dhea Kharisma and Ahmad Affan Ali Murtadlo and Devi Purnamasari and Nunuk Hariani Soekamto and ANM Ansori and Kuswati and Riso Sari Mandeli and Kawther Ameen Muhammed Saeed Aledresi and Nur Farhana Mohd Yusof and Vikash Jakhmola and Maksim Rebezov and Maksim Rebezov and Rahadian Zainul and Kiran Dobhal and Tarun Parashar and Muhammad Arya Ghifari and Deffi Ayu Puspito Sari} } @article {1849, title = {Leaf Effect of C. Trifolia L. as Nf-B and Tnf-Α Inhibitor Compounds with In Silico Method}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {407-415}, type = {Research Article }, chapter = {407}, abstract = {

Introduction: Infection H. pylori causes inflammation through various pathways to induce proinflammatory cytokines such as IL-1, IL-6, IL-8, and TNF-α. The transcription factor NF-kB is a crucial regulator of the immune response and inflammation and regulates many cellular processes that are important in carcinogenesis, including transformation, proliferation, angiogenesis, and metastasis. Antiinflammatory plant C. trifolia L was shown to inhibit the activity of NF-B and several pro-inflammatory cytokine mediators. This study proved that the active compound from the plant{\textquoteright}s leaves, C. trifolia L has potential as an inhibitor of NF-B and TNF-α. Method: This study used a docking method with a grid box mimicking the bond between the receptor and the inhibitor control complex. Results: The bioactivity of Cayratria trifolia compounds as anti-inflammatory was shown in the inflammation parameters used, namely Interleukin 10 agonist, Interleukin agonist, Interleukin antagonist, Interleukin 6 antagonist, Interleukin 4 antagonist, Interleukin 2 agonist, Interleukin 1 antagonist, Interleukin 1b antagonist, Interleukin 10 antagonist, Interleukin 12 agonist, and Interleukin 1a antagonist. Interleukin 2 agonists showed the highest activity of all compounds. Piceid compounds showed high anti-inflammatory activity with interleukin 10 agonists, interleukin agonists, interleukin 6 antagonists, and interleukin 2 agonists. The compounds stilbenes, piceid, resveratrol, cyclopentadecane, and hentriacontane showed potency higher interleukin-6 inhibition than the other 22 compounds. These five compounds were continued for molecular docking analysis. The low bond energy is correlated with the number of bonds and the variety of interactions. The higher the number of bonds and the type of interaction, the lower the bond energy. The lower the bond energy, the stronger the interaction between the ligand and protein. Conclusion: Based on the prediction of anti-inflammatory bioactivity, five potential compounds were identified, namely cyclopentadecane, resveratrol, stilbenes, piceid, and hentriacontane. The five compounds bind to NFkB on the active site of the binding site with DNA, and this inhibition causes DNA to be unable to restrain NFkB transcription factors, and transcription does not occur. This proves that the active compound from the leaves of the plant C. trifolia L has potential as an inhibitor of NF-κB compounds. Inhibition of 6 compounds on TNF at the TNF receptor proves that the active compound from the leaves of the plant C. trifolia L has potential as a TNF-α inhibitor compound. The active ingredient Piceid exhibits predominant anti-inflammatory potential with lower binding energy and stronger interactions than other complexes.

}, keywords = {C. trifolia L, H. Pylori, in silico, NFkB, TNF-α}, doi = {10.5530/pj.2022.14.115}, author = {Judya Sukmana and Widjiati and Siswandono and I Ketut Sudiana and Hari Basuki Notobroto and Iswinarno Doso Saputro and Yoes Prijatna Dachlan and Endang Joewarini} } @article {1712, title = {In silico Study on the Promising Active Components of Terpenoid and Fucoidon from Sargassum sp. in Inhibiting CGRP and TNF-α}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {December 2021}, pages = {1715-1719}, type = {Research Article}, chapter = {1715}, abstract = {

Introduction: The new discovery of the active substance in Sargassum sp marks the new era for drug industry as it is very effective as the new migraine medication compared to analgesics which have already been popular previously in treating migraine. By using the in silico methods, this study intended to identify the preventive effect of the active substance in Sargassum sp within the stage of pain and inflammation development in migraine. In migraine pathophysiology, the clinical findings would build and verify the role of CGRP and TNF-α. Methods: This research applied a one-shot experimental study and by employing the potential test through PubChem (https://pubchem.ncbi.nlm.nih.gov/), the result of this study proved that tannins, terpenoids and fucoidone were contained in the active substance of Sargassum sp leading to the possession of potential as the drug to treat migraine. Results: Terpenoids and tannin binding affinity value is higher than other substances. Terpenoids and fucoidon had similar amino acid residues with controls. Seaweed metabolites have great potential as inhibitors of CGRP and TNF-α because the binding affinity score is close to control. Conclusion: The active substance in Sargassum sp has an inhibitory effect on the occurrence of CGRP and TNF-α in migraine based on in silico studies.

}, keywords = {CGRP, Fucoidone, in silico, Sargassum sp., Terpenoid, TNF-α.}, doi = {10.5530/pj.2021.13.221}, author = {Olivia Mahardani Adam and Jusak Nugraha and Mohammad Hasan Machfoed and Agus Turchan} } @article {1296, title = {In silico Anticancer Activity and in vitro Antioxidant of Flavonoids in Plectranthus amboinicus}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {November 2020}, pages = {1573-1577}, type = {Original Article}, chapter = {1573}, abstract = {

Background: Plectranthus amboinicus (Lour.) Spreng is a plant that has a high flavonoid content. The leaves of Plectranthus amboinicus (Lour.) Spreng contain many flavonoids Chrysoeriol, Cirsimaritin, Eriodictyol, Luteolin, Rutin, Salvigenin, Thymoquinone, Quercetin, Apigenin, and 5-O-Methyl-Luteolin. Objectives: To determine the antioxidant activity and anticancer activity of flavonoid compounds contained in Plectranthus amboinicus (Lour.) Spreng. Methods: Anticancer activity testing was carried out by in silico against several cancer receptors and antioxidant activity testing was carried out by in vitro using the 1,1-Diphenyl-2-Picryhydrazil method. The results showed that the flavonoid compounds contained in Plectranthus amboinicus (Lour.) Spreng have similar anticancer activity to the reference molecule at the P-Glycoprotein-1, Cyclin Dependent Kinase-2, and Phosphoinositide-3-Kinase receptors, as well as better anticancer activity than the reference molecule for the Cyclooxygenase-2 and Phosphoenolpyruvate Carboxykinase receptors. Results: The antioxidant activity of the extract gave an Inhibitory Concentration 50\% value of 9.77 μg/mL, the flavonoid compounds contained in Plectranthus amboinicus (Lour.) Spreng gave an Inhibitory Concentration 50\% value that lower than the extract, which ranged from 6.92 μg/mL to 8.50 μg/mL. Flavonoids in Plectranthus amboinicus (Lour.) Spreng anticancer activity by in silico molecular docking and antioxidant activity by in vitro 1,1-Diphenyl-2-Picryhydrazil method. Conclusions: All the flavonoid compounds contained in the ethanolic extract of Plectranthus amboinicus (Lour.) Spreng leaves exhibit very strong anti-cancer and antioxidant activity, which results in ethanolic extract of Plectranthus amboinicus (Lour.) Spreng leaves have very strong antioxidant activity.

}, keywords = {Anticancer, Antioxidant, Flavonoid, in silico, in vitro}, doi = {10.5530/pj.2020.12.215}, author = {Kesaktian Manurung and Delmi Sulastri and Nasrul Zubir and Syafruddin Ilyas} } @article {625, title = {In silico, in vitro and in vivo Tests of Ficus deltoidea Jack Leaves Extract as Inhibitor for Beta-Catenin Expression in Colon Carcinogenesis Model}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {June 2018}, pages = {808-813}, type = {Original Article}, chapter = {808}, abstract = {

Context: Ficus deltoidea Jack leaves extract as anticolorectal cancer. Aims: This study aims to analyze the potential of FD extract to be an anti-colon cancer by investigating the extract capability in reducing \β-catenin expression and inhibiting colon cancer cells growth. Settings |and Design: The research was conducted in Medical Faculty Universitas Indonesia with experimental design. Methods and Material: FD ethanol extracts was tested in vitro, in silico and in vivo. In vitro test was conducted to human colon cell lines. In vivo test was conducted to Balb/c mice induced with 10 mg/kg azoxymethane (AOM) and dextran sodium sulfate 1\% (DSS). The colonic tissue collected was the distal portion. \β-catenin expressions in the cytoplasm and nuclei of the epithelial cells of the colon crypt were semi quantitatively assessed using the immunohistochemistry staining on ten visual fields with 400x magnification. Statistical analysis used: SPSS. Results: FD ethanol extracts inhibit the expression of \β-catenin in the crypt ephitelial cells of mice colon induced with AOM/DSS. The extracts also inhibit the growth of human colon cancer (HCT 116) with IC50 value of 5.41 mg/mL. Phytochemical screening to the extracts gave three groups of compounds: alkaloid, flavonoid, and tannin. Water fraction is the best fraction. Based on in the results of in silico analysis with molecular docking, FD extract is believed to influence the expression of \β-catenin, in which vitexin and isovitexin are the main candidate compounds to influence the expression of the protein. Conclusion: FD ethanol extract is potential to be an anti-colon cancer proven by the extract capability to reduce \β-catenin expression.

}, keywords = {Azoxymethane, Colon carcinogenesis, Ficus deltoidea, in silico, β-catenin}, doi = {10.5530/pj.2018.4.137}, url = {http://fulltxt.org/article/675}, author = {Kusmardi Kusmardi and Tedjo Aryo and Wuyung Puspita Eka and Fadilah Fadilah and Priosoeryanto Bambang Pontjo and Fachri Wilzar} }