@article {2116, title = {Anti-Neoplastic and Cytotoxicity Potency Measuring of Five Medicago sativa L. (Alfalfa) Leaf Extracts Towards Melanoma (UACC62), Breast (MCF7), Prostate (PC3), and Colon (HCT116) Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {October 2023}, pages = {768-776}, type = {Original Article}, chapter = {768}, abstract = {

The National Cancer Registry of the National Department of Health of South Africa confirms that in females, the highest incidence of cancers diagnosed in the year 2019 was breast (10 172 cases), cervical (6 945 cases) and colorectal (1 952 cases) cancers, while in males the most diagnosed cancers were prostate, lung, and colorectal neoplasms. The risk factors that promote cancer development, and metastasis or spreading includes excessive alcohol and narcotic usage, carcinogenic infections (oncoviruses) and genetical predispositions. The global incidence of cancer is much likely to increase because of the increased exposure to risk factors (processed fast foods, industrial toxins in air and water) that promote oxidative stress, low grade chronic inflammation, diabetes mellitus, hyperglycemia, and insulin resistance. Because the ancient healer or doctor relied on plants and other natural resources for therapeutic compounds, we wanted to measure the anti-cancer actions induced by our Alfac-facah leaf extracts towards four major cancer cells. The growth inhibitory and cytotoxicity activity that was induced by our extracts was measured using the reliable Sulforhodamine B Assay as per the collaborative research program between the CSIR{\textquoteright}s Biosciences Pharmacology Group and the NCI. Our Alfac-facah leaf test material was extracted using five solvents: Ethanol, Methanol, Diethyl-ether, Acetone, and Water. Medicinal Plants persist to play a key role in medicine, whereby they not only help with treating or preventing diseases, but they also contribute to the general wellbeing of the patient. For this reason, natural resources remain a pivotal ingredient of novel drug development compounds

}, keywords = {AICR (American Institute for Cancer Research), Cancer (malignancy, Carcinoma, CSIR (The Council for Scientific and Industrial Research, ECACC (European Collection of Authenticated Cell Cultures, International Agency for Research on Cancer (IARC), Leukemia, lymphoma), Medicago sativa L. (Alfalfa), NCI (National Cancer Institute, neoplasm, sarcoma, South Africa), Sulforhodamine B Assay (SRB), THC (delta-9 tetrahydrocannabinol). Rick Simpson oil (RSO)., Tumour, U.S Department of Health and Human Services), UK)}, doi = {10.5530/pj.2023.15.150}, author = {Makhele Thapelo Simon and Makhoahle Pakiso Moses and Mashele Sitheni Samson} } @article {531, title = {Andrographolide Induced Apoptosis in NALM-6 Cells Mediated Through the Cell Cycle Arrest and Nuclear Fragmentation}, journal = {Pharmacog Journal}, volume = {10}, year = {2018}, month = {January-2018}, pages = {210-214}, type = {Original Article}, chapter = {210 }, abstract = {

Introduction: Andrographis paniculata is an herb widely cultivated in South and Southeastern Asia. It has been traditionally used to treat infections and other Physiological disorders for several hundreds. We investigated the anti-leukemic potential of Andrographolide (AGP) isolated from the leaves of this plant against an array of cancer cells to investigate its most efficacies in a particular cancer type. Methods: AGP was isolated from Andrographis paniculata leaves by using column chromatography. The structure was further determined by LC-MS, 1H NMR and 13C NMR. AGP was initially tested against four different cancer cell lines, namely NALM-6 (pre B-ALL), K562 (CML), A549 (lung carcinoma) and MCF-7 (breast carcinoma) using MTT assay at different time points and different concentrations. The effect of the isolated biomolecule was also investigated in inducing apoptosis through the study of cell cycle progression using flow cytometry by PI staining and nuclear fragmentation pattern by DAPI staining and fluorescence microscopy. Results: the spectral analysis of the isolated bio-molecule assured that the compound was AGP. MTT assay data indicated that AGP was most potent to induce cytotoxicity in NALM-6 cells. Further investigation revealed that it effectively induced apoptosis by arresting cell cycle progression and increased the nuclear break down in NALM- 6 leukemic cells. Conclusion: Our study efficiently demonstrated that the AGP isolated from Andrographis paniculata induced apoptosis in NALM-6 cells, which could be used in the therapeutic intervention of leukemia in the future.

}, keywords = {Andrographis paniculata, Andrographolide, Apoptosis, Cell cycle, Cytotoxicity, Leukemia}, doi = {10.5530/pj.2018.2.36}, url = {http://fulltxt.org/article/466}, author = {Swadesh Sarkar and Priya K Gopal and Santanu Paul} }