@article {1250, title = {The Ability Free Radical Binding of Dengen{\textquoteright}s Stem Bark Extract (Dillenia serrata) From Luwu District Indonesia}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {September 2020}, pages = {1340-1345}, type = {Research Article}, chapter = {1340}, abstract = {

Degenerative diseases such as cancer, heart disease is one of the diseases caused by free radicals. Dengen (Dillenia serrata) is one of Indonesia{\textquoteright}s endemic local plants that has the potential to bind free radicals. So far, only limited to the use of fruit and consumed directly. This study aims to determine the ability of dengen stem bark extract to bind to free radicals expressed in IC50. In the initial research, the total content of polyphenols in dengen stem bark extract was 444.8 mg GAE / g or 44.48\%. Dengen stem bark is extracted by maceration using 70\% ethanol solvent. Antioxidant activity was calculated based on IC50 (Inhibition Concentration) values using the DPPH method, and measured at a wavelength of 516 nm. Ethanol extract of dengen bark is made in 5 concentration series, namely 10 ppm, 20 ppm, 30 ppm, 40 ppm and 50 ppm. Ethanol extract of dengen bark has a very active antioxidant activity with an IC50 value of 48.33 ppm. Whereas for comparison vitamin C has an IC50 value of 15.448 ppm. From the results, it can be concluded that the ethanol extract of dengen bark has the ability to bind free radicals with IC50 48.33 ppm

}, keywords = {Dengen, Ethanol Bark Extract, Free Radicals, IC50}, doi = {10.5530/pj.2020.12.184}, author = {Santi Sinala and Ismail Ibrahim and Alfrida Monica Salasa} } @article {1249, title = {The Effect of Carthamus tenuis Extracts on the Cell Proliferation of Different Tumor Cell Lines}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {September 2020}, pages = {1332-1339}, type = {Research Article}, chapter = {1332}, abstract = {

Background: Carthamus tenuis is one of the medicinal plants that was used traditionally to treat skin diseases, hemorrhoids, abortion, infertility. It also showed an immunosuppressive role as well as antifungal, antibacterial, anti-inflammatory activity. Although this plant is widespread, there are few studies about its medical applications. Objectives: This study was done to explore the anticancer activity of this plant. Materials and Methods: The aerial parts of the plant were dried, grinded and extracted with hexane, ethyl acetate, and methanol. The extracts were applied in different concentrations to cell cultures of breast (MCF-7), colon (HT-29), prostate (PC-3) and colorectal (CaCo-2) cell lines and fibroblast (MRC- 5) was used as a control. The anticancer activity was evaluated by 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium (MTT) reduction assay that was measured by spectrophotometer. Results: The results showed that methanol extract significantly (p\<0.05) have the highest inhibitory activity on MCF-7, HT-29, PC-3, and CaCo-2 with IC50; (25.52 μg/ml), (17.37 μg/ml), (25.77 μg/ml), (24.49 μg/ml), respectively. Followed by ethyl acetate extract that moderately inhibit cell growth of PC-3 and CaCo-2 with IC50; (28.99 μg/ml) and (21.45 μg/ml), respectively. n-hexane extract showed no significant inhibitory effect on all cell lines; IC50 (125.52 -152.34 μg/ml) when compared to Tamoxifen drug activity as a positive control. Conclusion: Results of this study showed the anticancer activity of the plant extracts in four different kinds of cancers that need further study.

}, keywords = {Anticancer, Carthamus tenuis, IC50, Methanolic extract, MTT}, doi = {10.5530/pj.2020.12.183}, author = {Maissa{\textquoteright} Taleb Shawagfeh} } @article {849, title = {ELISA Test on Cordia myxa L. Leaf Extract for alpha-Glucosidase Inhibitor}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {February 2019}, pages = {358-361}, type = {Original Article}, chapter = {358}, abstract = {

Aimed: Determine the potential of Cordia myxa L. leaf on inhibited α-glucosidase. Material: ELISA Kit, Ethanol 96\%, Colomn Chromatography, n-hexane, ethyl acetate, Glocobay{\textregistered}.\  Method: Sample from Cordia myxa L. leaf extracted by ethanol 96\% then evaporated to get the sticky extract. The sticky extract of Cordia myxa L. leaf fractionated by column chromatography with n-hexane, n-hexane: ethyl acetate (90:10; 80:20; 75:25; 70:30; 65:35; 60:40; 55:45; 50:50) Assay: The fractions assayed by ELISA (Enzyme-Linked Immunosorbent Assay) with acarbose (Glucobay {\textregistered}) as the comparator. Result: The results showed that the n-hexane fraction is the highest potency on inhibited α-glucosidase with the noncompetitive mechanism. The IC50 of n-hexane fraction is 0.53 ppm been while the acarbose is 6.85 ppm. Conclusion: The n-hexane fraction of Cordia myxa L. leaf has the highest potency to use for possible decrease blood glucose level.

}, keywords = {Acarbose, Cordia myxa L., ELISA, IC50, α-glucosidase}, doi = {10.5530/pj.2019.11.54}, author = {Ahmad Najib and Aktsar Roskiana Ahmad and Virsa Handayani} } @article {507, title = {Anthelmintic Activity of Fruit Extract and Fractions of Piper longum L. In vitro}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {January 2018}, pages = {333-340}, type = {Original Article}, chapter = {333}, abstract = {

The present study was aimed to assess the in vitro ovicidal, larvicidal and adulticidal activity of methanolic extract and its fractions from fruits of Piper longum against strongyle ova, larvae and adult amphistomes respectively. The fruits of P. longum was identified and the accession number 006 was obtained. The phytochemical analysis revealed the presence of tannins, flavonoids, glycosides, phenolics, diterpenes and triterpenes in extract and fractions of P. longum. The extract and fractions were diluted serially in 6.25 per cent tween 80 to obtain concentrations of 500, 250, 125, 62.5, 31.25, 15.63, 7.81, 3.91 and 1.95 mg/mL. Ivermectin and thiabendazole at 10 \μg/mL acted as positive controls and 6.25 per cent tween 80 as negative control. The methanolic extract was highly active against ova with IC50 of 0.026 mg/mL. The n-hexane fraction was potent in inducing larval mortality with IC50 of 1.383 mg/mL while chloroform fraction inhibited larval migration with IC50 of 1.796 mg/mL. Amphistomes were highly sensitive for methanolic extract of P. longum which possessed IC50 of 5.493 mg/mL Based on IC50 values, the methanolic extract was found to be most potent while chloroform fraction was effective against ova, larvae and also adults. GCMS analysis of potent methanolic extract revealed the presence of piperidinone, hydrocinnamic acid, ethylhexahydro azepine, methyleugenol, hexadecanoic acid and caryophyllene oxide which may have contributed for the anthelmintic activity. The acute oral toxicity study revealed mild vascular changes in liver. From the present study, it can be concluded that chloroform fraction of P. longum possessed maximum broad spectrum anthelmintic activity comparable to controls.

}, keywords = {Adulticidal, GCMS, IC50, Larvicidal, Ovicidal, Piper longum.}, doi = {10.5530/pj.2018.2.57}, url = {http://fulltxt.org/article/487}, author = {Krishnaprasad Ganapati Koorse and Sujith Samraj and Preethy John and Priya Manakkulaparambil Narayanan and Devi SS and Usha PTA and Surya Sunilkumar and Gleeja VL} }