@article {1118, title = {Anti-Elastase, Antioxidant, Total Phenolic and Total Flavonoid Content of Wuru Ketek (Myrica javanica Reinw. ex Bl.) from Tangkuban Perahu, West Java - Indonesia}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {293-297}, type = {Original Article}, chapter = {293}, abstract = {

Introduction: The presence of elastase and ROS can cause skin aging, phenolic compounds and flavonoids can be used to inhibit elastase activity and as an antioxidant. Objective: This research aimed to evaluate the anti-elastase, antioxidant activities, TPC and TFC of extracts from Myrica javanica. Methods: In this study, the leaves, stems and fruit of Myrica javanica were macerated with 96\% ethanol. The extracts obtained were analysed for anti-elastase and antioxidant activities. It was also evaluated for TPC and TFC. Result: IC50 anti-elastase on leaves extract (LE), stems extract (SE), and fruits extract (FE) respectively showed 64.71 ppm, 197.49 ppm, and no activity. The anti-elastase result of three extracts are lower if compared with Myricetine (9.54 ppm). SE showed highest DPPH and TPC value (IC50=16.36 μg/mL; 307.00 mgGAE/g dry weight) and LE showed highest FRAP and TFC value (421.68 Mol/gram; 15.80 mgQE/g dry weight). Conclusion: In summary, anti-elastase and antioxidant activity are influenced by differences in the content of compounds in the extract.

}, keywords = {Anti-Elastase, Antiaging, Antioxidant, Myrica javanica, TFC, TPC}, doi = {10.5530/pj.2020.12.46}, author = {Varda Arianti and Berna Elya and Iskandarsyah} } @article {1289, title = {In vitro Propagation and Determination of Total Phenolic Compounds, Flavonoid Contents and Antioxidative Activity of Globba globulifera Gagnep}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {November 2020}, pages = {1740-1747}, type = {Research Article}, chapter = {1740}, abstract = {

Introduction: Currently, there is a reduction in the number of Globba globulifera, which is due to its slow multiplication rate, high susceptibility to pathogenic diseases and overexploitation of the plant from natural sources. In vitro culture to study suitable concentrations of plant growth regulators for shoot and root induction of G. globulifera. Bioactive compounds were measured by TPC, TFC and FRSA methods for comparison of those from in vitro and natural conditions. Methods: Microshoots were cultured on solid and liquid MS medium supplemented with various concentrations of cytokinins (BA, Kinetin and TDZ) and auxins (NAA and IBA) for eight weeks. Methanol was used as the extraction solvent via the ultrasonic method, TPC and TFC were both measured. DPPH for free radical scavenging activity was investigated. Results: The best result for shoot formation was achieved when culturing on MS medium with 3 mg/l and 5 mg/l of BAP or 5 mg/l of BAP plus 1 mg/l of IBA. The plantlets were transplanted to pots in a greenhouse. All the planting material showed a 100\% survival rate. The rhizomes of in vitro derived plantlets showed the highest value of TPC (52.28 mg GAE/g crude extract) and FRSA (93.55\%) and lowest of IC50 (0.46 mg/ml). Conclusion: The in vitro culture and antioxidant activity analysis could be the foundation for plant propagation in large quantities and the use of medicine.

}, keywords = {Bioactive compound, DPPH, Micropropagation, TFC, TPC, Zingiberaceae}, doi = {10.5530/pj.2020.12.236}, author = {Wipa Yaowachai and Surapon Saensouk and Piyaporn Saensouk} } @article {484, title = {Phytochemical Profile and Antioxidant Activity of Propolis Ethanolic Extract from Tetragonula Bee}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {December 2017}, pages = {128-135}, type = {Original Article}, chapter = {128}, abstract = {

Objective: This research aimed to determine the phytochemical composition and the antioxidant properties in vitro of three types propolis collected from the region of South Sulawesi Province of Indonesia. Methods: Samples from three types of propolis: smooth, rough and mix were extracted with 96\% ethanol. The propolis ethanolic extracts (PEE) was dried and studied their antioxidant properties by using FRAP (Ferric Reducing Antioxidant Power) and DPPH radical scavenging assays. Total phenolic compounds were quantified by Folin-Ciocalteu, and total flavonoid contents were also quantitatively determined by the AlCl3 colorimetric method with a microplate reader. The chemical compounds were identified by an ultraperformance liquid chromatography TOF mass spectrometer (UPLC-TOF-MS) using the MS mode. Results: The sequence of potential antioxidant activity of PEE is smooth propolis \> mix propolis \> rough propolis. Which showed by EC50 value with DPPH assays are 25.54; 31.66; and 69.96 \μg/mL, respectively and also showed by EC50 value with FRAP assays consecutively were 26.41; 32.10; and 34.62 \μg/mL.Smooth propolis has the lowest EC50 value of all the types of propolis examined, contains total flavonoid content 791.06+13.06 mg QE/g extract and total phenolics content 426.91+61.08 mg GAE.g-1 extract. Chemical component identified by UPLC-TOF-MS using the MSE mode were (-)-Sesamin C20H18O6; Curcumin C21H20O6; 8-epi- Helenalin C15H18O4; and Kushenol F C25H28O6. Conclusion: Smooth propolis which taken from inside the nest was the most potent antioxidant among of all the types of examined propolis. The antioxidant activity was influenced by the phenolic content of Propolis.

}, keywords = {DPPH, FRAP, Microplate method, TFC, TPC, UPLC-TOF-MS}, doi = {10.5530/pj.2018.1.23}, url = {http://fulltxt.org/article/409}, author = {Diah Kartika Pratami and Abdul Mun{\textquoteright}im and Andini Sundowo and Muhamad Sahlan} }