@article {1695, title = {In vitro Wound Healing Potential and Antimicrobial Activity of Clerodendrum Inerme Leave Extracts}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1542-1548}, type = {Review Article}, chapter = {1542}, abstract = {

Background: Clerodendrum inerme is a medicinal plant which exhibited many pharmacological effects. However, wound healing property of this plant has not been investigated. Objective: The present study was designed to evaluate wound healing and antimicrobial activities of C. inerme leaves using the in vitro model with Human keratinocytes (HaCaT). Methods: Cytotoxicity and wound healing effects were determined by MTT and scratch wound healing assay. Antimicrobial activities against cutaneous flora and clinical isolates of bacteria were investigated by broth microdilution assay. Results: The results showed that the water extract of C. inerme leaves did not exhibit cytotoxic effects on HaCaT cells, while ethanol extracts at higher concentrations significantly decreased cell growth with an IC50 value of 386.8 {\textpm} 87.1 μg/mL. The water extract and the lowest concentration (6.25 μg/mL) of the ethanol extract significantly increased percentage of wound closure compared with the untreated group. The water and ethanol extracts of C. inerme displayed a broad spectrum of antibacterial activity, inhibiting growth of Staphylococcus aureus, Staphylococcus.epidermidis, Escherichia coli, and Pseudomonas aeruginosa. The water extract displayed remarkable activity against methicillin-resistant S. aureus with MIC and MBC values ranging from 0.39 to 1.56 μg/mL. Notably, it provided stronger antibacterial activity than vancomycin and also showed antifungal activity against C. albicans. Conclusion: This study confirms the potential of C. inerme leaves for wound healing and antimicrobial therapy and supports the continued utilization of C. inerme leaves in traditional medicine. Further studies are needed to clarify the molecular mechanisms through which it exerts such biological effects.

}, keywords = {Antimicrobial activity, Clerodendrum inerme, Keratinocytes, Scratch assay, Wound Healing}, doi = {10.5530/pj.2021.13.196}, author = {Sueptrakool Wisessombat and Malatee Tayeh} } @article {460, title = {Aristolochia bracteolata Enhances Wound Healing in vitro through Anti-inflammatory and Proliferative Effect on Human Dermal Fibroblasts and Keratinocytes}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {November 2017}, pages = {s129-s136}, type = {Original Article}, chapter = {s129}, abstract = {

Objective: In the present study, we examined the effect of Aristolochia bracteolata extract on Human dermal fibroblast (HDF) and Human keratinocyte cell line (HaCaT) proliferation and migration during in vitro wound healing and its underlying mechanism. Method: A. bracteolata was collected and extracted using methanol. Cytotoxiciy effect of plant extract was determined by MTT assay in HDF and HaCaT. In vitro Scratch assay determined the effect of plant extracts on migration of cells and its underlying mechanism was determined by RT-PCR analysis. Result: The plant extract of A. bracteolata selectively inhibited proliferation of both the cells at higher concentration (\>100 \μg/mL) and at lower concentrations (\<25 \μg/mL), it exhibited linear and dose-dependent cell proliferation. IC50 value was 87.60\±1.67 \μg/mL for HDF and 85.50\±1.65 \μg/mL after 24 h treatment. In vitro scratch wound healing studies showed wound closure of 50.38\%\±1.39 and 69.81\%\±1.89 at a concentration of 25 \μg/mL after 24 h and 48 h, respectively. The extract was tested for anti-inflammatory activity by determination of inhibitory activity on lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW 264.7 cell lines. We found that A. bracteolata has a strong inhibitory effect on the production of NO and tumor necrosis factor-\α (TNF-\α). The plant extract of A. bracteolata inhibited inducible nitric oxide synthase (iNOS) gene expression by lipopolysaccharide (LPS). To explore the mechanism responsible for the inhibition of iNOS, gene expression was analyzed by Real- Time PCR. A. bracteolata showed a decrease in the expression of pro-inflammatory cytokine mRNA in a concentration-dependent manner. Treatment with the plant extract resulted in enhanced expression of Collagen 1 a (I) and Collagen IV in HDFs by regulating the mRNA levels of extracellular matrix (ECM) proteins and Matrix metalloproteinase-2. Conclusion: Thus, the present investigation scientifically validates the use of A. bracteolata in wound healing.

}, keywords = {A. bracteolata, Fibroblast, Keratinocytes, RAW 264.7, Scratch assay, Wound Healing}, doi = {10.5530/pj.2017.6s.169}, url = {http://fulltxt.org/article/394}, author = {Dinesh Murugan Girija and Mangathayaru Kalachaveedu and Rajasekaran Subbarayan and Preethi Jenifer and Suresh Ranga Rao} }