@article {1166, title = {Assessment of the Impact of Wild Stinkhorn Mushroom Extracts on Different Cancer Cell Proliferation and Study of Primary Metabolites}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {June 2020}, pages = {699-708}, type = {Original Article}, chapter = {699}, abstract = {

Objective: Present study aims to evaluate the efficacy of methanolic and ethyl acetate extracts of wild mushroom Phallus sp. on cell proliferation of both normal and cancer cells. This study also looked at anti-oxidant potentiality of methanolic extract and also unravels the phytochemical profiling of both extracts. Methods: Anti-proliferative activity was assessed by MTT assay on different human cancer cell lines such as MCF-7, MOLT-4, REH and Peripheral Blood Mononuclear Cells or PBMC isolated from a healthy donor. Gas Chromatography-Mass Spectrometry (GC-MS) analysis was used for comparative assessment of phytochemical constituents of both extracts. The anti-oxidant profile of methanolic extract was also evaluated by DPPH and ABTS{\textbullet}+ assays. Results: Results indicated that the both methanolic and ethyl acetate extracts of Phallus sp. showed appreciable anti-proliferative activity against breast cancer cell line MCF-7 with IC50 of 8.544{\textpm}2.812 μg/mL and 35.279{\textpm}2.863 μg/mL respectively. Both of the extracts also showed its moderate impact on human B cell precursor leukemia cell line (REH) with IC50 of 25.987{\textpm}2.696 μg/mL for methanol and 51.484{\textpm}1.480 μg/mL for ethyl acetate extract respectively. No effect was observed in MOLT-4 cell line. Methanolic extract was selected as better anti cancer extract over ethyl acetate extract. No significant anti-proliferative activity was observed in normal PBMC by both extracts. GC-MS analysis indicated that 43 and 114 compounds were identified from methanolic and ethyl acetate extracts respectively. Among them nine compounds shared its existence in both of the extracts. Different derivatives of ergosterol and several fatty acid esters ware identified as major components from both of the extracts. Methanolic extracts of the Phallus sp. showed its effectiveness on both of DPPH and ABTS{\textbullet}+ free radical, and result indicated that it contain more flavonoid content than phenol. Conclusion: The methanolic extract of Phallus sp. show very specific anti-proliferative effect on MCF-7 with moderate anti-oxidant activity and holds a great promise for isolation of bio molecules for treating Breast Cancer. Several derivatives of ergosterol identified as probable anti-cancer compound.

}, keywords = {ABTS{\textbullet}+, GC-MS, MCF-7, MTT Assay, Phallus}, doi = {10.5530/pj.2020.12.102}, author = {Ribhu Ray and Amrita Pal and Santanu Paul} } @article {57, title = {Dynamic Comparison of Free Radical Scavenging Abilities of Hypericum Perforatum L., Herba Verbenae Officinalis, and Valeriana Officinalis L. Extracts}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {01/2015}, pages = {198-204}, type = {Original Article}, chapter = {198}, abstract = {

Objectives: The purpose of this research was to compare the dynamic antioxidant activities and the free radical scavenging abilities of three botanical supplements, Hypericum perforatum L. (HPL), Herba Verbena Officinalis (HVO), and Valeriana officinalis L. (VO), which have been reported to effectively treat menopause symptoms. Methods: The antioxidant activities of the three supplements were determined by the ferric reducing ability of plasma (FRAP) assay. In addition, their free radical scavenging abilities were studied by ftheir interactions with the stable radicals of 2, 2\’-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). Results: The results indicated that the HPL ethanolic extract exhibited greater antioxidant activity (1.2688 \± 0.01 mmol Trolox/g) than the HVO ethanolic extract (1.1686\± 0.043 mmol Trolox/g) and the VO ethanolic extract (0.2579 \± 0.0031 mmol Trolox/g) as compared to Nilestriol (0.0026\±0.0012), a positive control agent in the experiment. Moreover, the HPL extract showed remarkable free radical scavenging activity against ABTS\•+, and the HVO extract was the most potent against DPPH\•. Conclusion: These activities may be attributed to the total flavonoid or phenolic acid contents of among these extracts.

}, keywords = {ABTS{\textbullet}+, DPPH{\textbullet}, Herba Verbena Officinalis, Hypericum perforatum L., Menopause Oxidative Stress, Valeriana officinalis L.}, doi = {10.5530/pj.2015.3.9}, author = {Yi-ming Li and Yan-Ru Liu and Bing-Kun Xiao and Jian-Yun Yang and Rong-Qing Huang} } @article {1476, title = {Dynamic Comparison of Free Radical Scavenging Abilities of Hypericum Perforatum L., Herba Verbenae Officinalis, and Valeriana Officinalis L. Extracts}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {09th Mar, 2015}, pages = {198-204}, type = {Original Article}, chapter = {198}, abstract = {

Objectives: The purpose of this research was to compare the dynamic antioxidant activities and the free radical scavenging abilities of three botanical supplements, Hypericum perforatum L. (HPL), Herba Verbena Officinalis (HVO), and Valeriana officinalis L. (VO), which have been reported to effectively treat menopause symptoms. Methods: The antioxidant activities of the three supplements were determined by the ferric reducing ability of plasma (FRAP) assay. In addition, their free radical scavenging abilities were studied by ftheir interactions with the stable radicals of 2, 2\’-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). Results: The results indicated that the HPL ethanolic extract exhibited greater antioxidant activity (1.2688 \± 0.01 mmol Trolox/g) than the HVO ethanolic extract (1.1686\± 0.043 mmol Trolox/g) and the VO ethanolic extract (0.2579 \± 0.0031 mmol Trolox/g) as compared to Nilestriol (0.0026\±0.0012), a positive control agent in the experiment. Moreover, the HPL extract showed remarkable free radical scavenging activity against ABTS\•+, and the HVO extract was the most potent against DPPH\•. Conclusion: These activities may be attributed to the total flavonoid or phenolic acid contents of among these extracts.

Key words: ABTS\•+, DPPH\•, Hypericum perforatum L.,Herba Verbena Officinalis, Menopause Oxidative Stress, Valeriana officinalis L.

}, keywords = {ABTS{\textbullet}+, DPPH{\textbullet}, Herba Verbena Officinalis, Hypericum perforatum L., Menopause Oxidative Stress, Valeriana officinalis L.}, author = {Yi-ming Li and Yan-Ru Liu and Bing-Kun Xiao and Jian-Yun Yang and Rong-Qing Huang} }