@article {345, title = {Apoptosis Induction of Cervical Carcinoma HeLa Cells Line by Dichloromethane Fraction of the Rinds of Garcinia cowa Roxb}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {May 2017}, pages = {475-478}, type = {Original Article}, chapter = {475}, abstract = {

Objective: This study aims to determine the apoptosis induction of HeLa cervical carcinoma cells death by dichloromethane fraction of the rinds of Garcinia cowa Roxb. Methods: Apoptosis induction of HeLa cell line was observed using a double staining method. Results: The result of double staining observation showed that an apoptosis occurs which marked with yellowish green fluorescence and cell fragmentation. The average percentage of apoptotic cells was higher in the treated variables (70.38\%) compared to the control variables (12.26\% ). Statistical analysis by Independent Sample T-Test showed apoptosis Sig. (2-tailed) = 0.000 (\<0.025). Conclusion: The dichloromethane fraction of G. cowa rind induces apoptosis in HeLa cervical cancer cells.

}, keywords = {Apoptosis induction, Carcinoma cells, Double stanining, Garcinia cowa Roxb., HeLa cell line}, doi = {10.5530/pj.2017.4.76}, url = {/files/PJ-9-4/10.5530pj.2017.4.76}, author = {Fatma Sri Wahyuni and Siska Febria and Dessy Arisanty} } @article {1500, title = {Antiproliferative activity of crude extract and fractions obtained from Digera muricata on Hela cell lines of human cervix and A549 cell lines of Human Lung.}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {3rd Sept, 2014}, pages = {32-38}, type = {Original Article}, chapter = {32}, abstract = {

Digera muricata (Linn.) of family Amaranthaceae is an ethanobotanically important plant species traditionally used against various disorders. Cytotoxic potential of methanolic extract and its fractions were investigated against Hela and A549 cell lines. Crude extract of Digera muricata was prepared in methanol by Continuous Hot Soxhlation technique. Crude extract was fractionated into two organic and one aqueous fraction by the help of Column Chromatography. MTT assay was used to evaluate the reduction of viability of the cancer cell lines. Cell viability was inhibited by crude extract of Digera muricata in a dose dependent manner ranging from 25\μg/ml to 250\μg/ml. Apoptosis assays using nucleic acid stains namely PI exclusion assay and Hoestch/PI assay were performed by the help of fluorescence microscopy. Morphological analysis was done by calculation of Apoptotic ratio and Percentage apoptosis. Our results suggests that methanolic and aqueous fraction of the extract of Digera muricata can be good source of cytotoxic compounds.

Key words: 3-(4,5-dimethylthiazol-2yl)-2,4 diphenyltetrazolium bromide assay, A549 cell line, cytotoxic, Digera muricata, HeLa cell line.

}, keywords = {3-(4, 4 diphenyltetrazolium bromide assay, 5-dimethylthiazol-2yl)-2, A549 Cell Line, Cytotoxic, Digera muricata, HeLa cell line}, author = {Shazia Usmani and Arshad Hussain and A.H.A Farooqui and Mohd.Arshad and Sahabjada Siddiqui and Mohd.Ahmad and Shadma Wahab} }