@article {1167, title = {Isolation, Identification Similarity and Qualitative Expression of Metallothionein Gene in IR-Bagendit Rice (Oryza sativa)}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {June 2020}, pages = {709-715}, type = {Original Article}, chapter = {709}, abstract = {

Metallothionein (MTs) is an enzyme that plays a role in the binding of metals in plants. Various types of rice have been known to contain MTs and IR-Bagendit rice leaves have the highest MTs protein content compared to other rice varieties. However, MTs coding gene in IRBagendit rice variety is still unknown. OsRAC1 gene is reported as the down-regulator of MTs and there is an analogous gene for MTs-like gene using RAP1 and RAP2 primers in various plants. This study aimed to isolate, identification of similarity, and analysis of qualitative expression of MTs gene in IR-Bagendit rice as compared to Inpari, IR-36, and IR-34. The steps of this research were DNA isolation, PCR in OsRAC1 gene, RNA isolation and cDNA reverse transcription using primer RP1 and RP2, and agarose gel electrophoresis. Amplification quality of OsRAC1 gene in IR-Bagendit, Inpari, IR-36, and IR-34 showed the same result. Qualitative expression of MTs by reverse transcription showed that IR-Bagendit has the highest MTs-like gene compared to other samples.

}, keywords = {IR-Bagendit, Metal Exposure, Metallothionein, MTs-like gene, Rice}, doi = {10.5530/pj.2020.12.103}, author = {Budi Santosa and Budi Santosa and Aprilia Indah Kartika and Fitri Nuroini and Aditya Rahman Ernanto and Annisa Ayuningtyas and Mohd Nazil Salleh and Siti Thomas Zulaikhah} } @article {334, title = {Antioxidant Activity, Biochemical Components and Sub-Chronic Toxicity of Different Brown Rice Kefir Powders}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {April 2017 }, pages = {388-394}, type = {Original Article}, chapter = {388}, abstract = {

Aims: This study aimed to investigate and compare antioxidant activity, biochemical components and subchronic toxicity of brown rice kefir powder from three different coloured rice varieties; Khao Dawk Mali 105 (KDMLKP, white), Red Hawm (RHKP, red), and Hawm Nil (HNKP, dark purple), with cow milk kefir powder (CMKP). Medthods: Antioxidant activity performed by using ferric reducing antioxidant power (FRAP) assay and 2, 2\´-diphenyl-1-picrylhydrazyl (DPPH) assay. Biochemical components values including gamma-aminobutyric acid and alpha-tocopherol were determined using high performance liquid chromatography, but total phenolic compounds was detemined using the Folin-Ciocalteu reagent. Sub-chronic toxicity test, KDMLKP, RHKP and HNKP at the doses of 150 mg/kg were given orally to the rats every day for 90 days. Results: Antioxidant activity showed that brown rice kefir powders were significantly (p\≤0.05) higher than cow milk kefir powder. Interestingly, HNKP provided the highest FRAB value and the lowest IC50 of DPPH assay. HNKP had also the highest biochemical component values. Repeat administration of KDMLKP, RHKP and HNKP did not produce any symptoms of sub-chronic toxicity; mortality, hematological values, and blood biochemistry in the rats. Moreover, RHKP produced HDL increasing without CHO, TG and LDL changing in rats. Conclusions: These data indicate that brown rice kefir powders show no sub-chronic toxicity and exhibit more antioxidant activity and biochemical component values than cow milk kefir powder. Surprisingly, RHKP showed HDL increasing while HNKP had the highest potent antioxidant activity and biochemical components. Thus, both rice kefir powders are safe and probably a new biochemical resource for health.

{\textquoteleft}}, keywords = {antioxidant activity, Biochemical Components, Brown Rice Kefir Powder, Rice, Sub-Chronic Toxicity}, doi = {10.5530/pj.2017.3.66}, url = {/files/PJ-9-3/10.5530pj.2017.3.66}, author = {Supaporn Chunchom and Chusri Talubmook and Sirirat Deeseenthum} }