@article {345, title = {Apoptosis Induction of Cervical Carcinoma HeLa Cells Line by Dichloromethane Fraction of the Rinds of Garcinia cowa Roxb}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {May 2017}, pages = {475-478}, type = {Original Article}, chapter = {475}, abstract = {

Objective: This study aims to determine the apoptosis induction of HeLa cervical carcinoma cells death by dichloromethane fraction of the rinds of Garcinia cowa Roxb. Methods: Apoptosis induction of HeLa cell line was observed using a double staining method. Results: The result of double staining observation showed that an apoptosis occurs which marked with yellowish green fluorescence and cell fragmentation. The average percentage of apoptotic cells was higher in the treated variables (70.38\%) compared to the control variables (12.26\% ). Statistical analysis by Independent Sample T-Test showed apoptosis Sig. (2-tailed) = 0.000 (\<0.025). Conclusion: The dichloromethane fraction of G. cowa rind induces apoptosis in HeLa cervical cancer cells.

}, keywords = {Apoptosis induction, Carcinoma cells, Double stanining, Garcinia cowa Roxb., HeLa cell line}, doi = {10.5530/pj.2017.4.76}, url = {/files/PJ-9-4/10.5530pj.2017.4.76}, author = {Fatma Sri Wahyuni and Siska Febria and Dessy Arisanty} } @article {326, title = {Sub-acute Toxicity Study of The Ethyl Acetate Fraction of Asam Kandis Rinds (Garcinia cowa Roxb.) on the Liver and Renal Function in Mice}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {April 2017 }, pages = {345-349}, type = {Original Article}, chapter = {345}, abstract = {

Objective: The present study investigated the sub acute toxicity of the ethyl acetate fraction of asam kandis (Garcinia cowa Roxb) Rinds in mice. Material and Methods: Sub acute toxicity study was carried out by giving orally at dose 500, 1000 dan 2000 mg / kgBW extract to five mice at 21 days. Animals were observed individually for any clinical signs of toxicity or mortality for 14 days. Measured parameters were SGPT levels, serum creatinine levels, weight ratio of liver and kidney. Extract was given orally at dose 500, 1000 and 2000 mg/kgBW for 21 days. Observations were done on day 8th, 15th and 22th using blood serum, liver and kidneys of mice. Data were analyzed by using two-way ANOVA followed by Duncan\’s Multiple Range Test. Results: The ethyl acetate fraction of G. cowa at doses 500, 1000 and 2000 mg/kgBW gave significant effect on increasing SGPT levels and decreasing levels of serum creatinine (p \<0.05). The length of treatment gave significant effect on decreasing levels of serum creatinine, weight ratio of liver and kidney (p \<0.05). Conclusion: The dosage of the ethyl acetate fraction of asam kandis rinds provides significant effect on the SGPT and serum creatinine levels of male white mice. The duration of administration of ethyl acetate fraction of asam kandis rinds provides significant effect on serum creatinine levels, the weight ratio of liver and kidney organ of male white mice.\ 

}, keywords = {Creatinine serum, Garcinia cowa rinds, SGPT, Sub-acute toxicity, Weight ratio of liver and kidney}, doi = {10.5530/pj.2017.3.58}, url = {/files/PJ-9-3/10.5530pj.2017.3.58}, author = {Fatma Sri Wahyuni and Dessy Arisanty and Nelsi Fitri Hayaty and Dian Ayu Juwita and Almahdy} }