@article {642, title = {In vitro Antifungal Effects of Hesperetin and Silibinin}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {June 2018}, pages = {789-792}, type = {Original Article}, chapter = {789}, abstract = {

Background: Increasing resistance of microorganisms against available antimicrobial agents is of major concern among scientists and clinicians worldwide. To overcome the resistance and other drawbacks of the current antimicrobial drugs and to obtain more efficacious drugs, an antimicrobial drug having a novel mode of action should be developed. The flavonoid compounds have demonstrated for their therapeutic potential as antimicrobial agents previously. Aim: To evaluate the antifungal efficacy of plant derived compounds such as hesperetin (HSP) and silibinin (SBN) compounds in vitro. Materials and Methods: In this study, we evaluated the antifungal effect of two plant derived flavonoid compounds i.e., SBN and HSP against Candida albicans, Candida tropicalis, Malassezia furfur, Aspergillus niger, Penicillium oxalicum. The disk diffusion test was performed to test the antifungal effect of HSP and SBN with three different concentrations i.e., 15, 20, 25 mg/ml of HSP and SBN. The antifungal efficacy was studied for 72 h. Results: Our results indicate that SBN has significant antifungal effect than that of HSP. The high sensitivity of these compounds was found against C. albicans, C. tropicalis. Conclusion: These drugs could be good candidate to combine with regular antimicrobial agents.

}, keywords = {Candida albicans, Flavonoids, Fungal infection, Hesperetin, Silibinin}, doi = {10.5530/pj.2018.4.133}, url = {http://fulltxt.org/article/671}, author = {Ramar Gowtham and Fysal Yousuf MA and Devaraj Ezhilarasan and Shanmugam Sambantham and Balakrishnan Anandan} } @article {252, title = {Cytotoxic Effect of Caralluma fimbriata Against Human Colon Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {204-207}, type = {Original Article}, chapter = {204}, abstract = {

Aim: The present study was designed to examine the cytotoxic effects of ethanolic leaf extract of Caralluma fimbriata in the COLO 320 cell line. Materials and Method: The anti-proliferative effects were evaluated using the MTT assay. The COLO 320 cells were treated with different concentrations of the leaf extract of Caralluma (100 \– 300 \μg/ml) for 24 h. The cell viability and IC50 was calculated from the cytotoxicity. The morphology of the Caralluma treated cells, control, and positive control were observed under reverse phase inverted microscope. Result: The C. fimbriata ethanolic leaf extract showed dose dependant increase in cytotoxicity in COLO 320 human colon cancer cells. The maximum cytotoxic effect was noticed with maximum dose used in this study i.e., 300 g with an IC50 value of 233.87 g. Conclusion: The present study shows that the ethanolic leaf extract of Caralluma fimbriata is capable of reducing cell proliferation by inducing cytotoxicity of COLO 320 cells.

}, keywords = {Caralluma fimbriata, COLO 320 cell line, Colonic cancer, Cytotoxicity, MTT Assay}, doi = {10.5530/pj.2017.2.34}, url = {http://phcogj.com/fulltext/301}, author = {Shenai Ashwini and Devaraj Ezhilarasan and Roy Anitha} }