@article {1654, title = {Microwave-Assisted Extraction and Identification of γ- Oryzanol from Rice Bran (Oryza sativa L. cv Ciliwung)}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {September 2021}, pages = {1242-1247}, type = {Research Article}, chapter = {1242}, abstract = {

Rice Bran (Oryza sativa L. cv Cilliwung) is a by-product of the rice milling process, consisting of an outer layer of rice grains with the whole seed institute. Rice bran oil is a result of rice bran extraction. Rice bran oil contains antioxidant compounds, one of which is oryzanol, so this study aims to determine the levels of or-oryzanol in rice bran. The stages of the research included sample preparation, extraction using Microwave-Assisted Extraction (MAE), followed by Vacuum Liquid Column Chromatography (VLCC) was extracted using the MAE method produced 30 mL of black-brown rice bran oil extract. A total of 8 grams of rice bran extract was obtained at VLCC using eluent (n-hexane: ethyl acetate) with ratios 9:1, 7:3, and 1:1, respectively. Then the five factions obtained were characterized using HPLC, and GC/MS identified the mass of the γ-oryzanol compound.

}, keywords = {Microwave assisted extraction, Rice Bran (Oryza sativa L. cv Cilliwung), Secondary metabolite, γ- Oryzanol}, doi = {10.5530/pj.2021.13.157}, author = {Maryono and Netti Herawati and Meuthia Aulia Farhani Gaffar and Sartini and Aliyah and Elly Wahyudin} } @article {246, title = {Lipid Peroxidation Inhibitory Activity In vitro of Mezzetia parviflora Becc. Wood Bark Polar extract}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {171-175}, type = {Original Article}, chapter = {171}, abstract = {

Introduction: The wood bark of Mezzetia parviflora Becc, has long served as one of the most important traditional herbal medicine sources in Buton Regency, Southeast Sulawesi. M. parviflora extracts were rich in polyphenols. This study was aimed to explore the lipid peroxidation inhibitory activity of polar extract of M. parviflora. Methods: The polar extract is the result of ethanol extract partition solved in acetone. The extract will keep polar components which are insoluble in acetone. Assayed methods applied are \ß-carotene bleaching inhibition, thiobarbituric acid reactive substance (TBARS) measurement, and continuous monitoring of conjugated dienes formation in LDL. Results: M. parviflora extract inhibit \ß-carotene/ linoleic acid oxidation, showed by IC50 value of 15.83 g/ml in 30th minute; but the potency will be reduced to IC50 value of 111.19 g/ml and 225.07 g/ml after the 60th and 120th minute of incubation. M. parviflora extract inhibit MDA formation as for linoleic acid peroxidation product until the third day; at 20, 40, 60, 80 and 100 g/ml inhibit MDA formation as many as 29.16 \± 2.41\%, 4.24\% \± 43.27, 54.08 \± 2.87\%, 59.88 \± 1.90\%, and 69.75 \± 2.32\%, respectively. M. parviflora extract at 50 g/ml can inhibit LDL-oxidation induced by CuSO4, performed by LDL-oxidation lag-time elongation until 70 minutes, similar ability was performed by epigallocathecin gallate at 5 g/ml. Conclusions: M. parviflora extract expressed relatively strong protection against lipid and LDL oxidation which can serve as the scientific basis of its development as a remedy for various diseases caused by lipid peroxidation.

}, keywords = {Conjugated diene, Low-density lipoprotein, Malondilaldehyde, Mezzetia parviflora Becc, Polyphenol}, doi = {10.5530/pj.2017.2.28}, url = {http://phcogj.com/fulltext/295}, author = {Mufidah Murdifin and Ermina Pakki and Gemini Alam and Marianti A. Manggau and Lukman Muslimin and M. Rusdi and Elly Wahyudin} }