@article {553, title = {Effect of Thai Folklore Recipe from Abutilon indicum and Mimosa pudica in Streptozotocin-Induced Diabetic Rats}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {x}, pages = {x}, type = {x}, chapter = {x}, abstract = {

Context: Abutilon indicum and Mimosa pudica were a folklore recipe in Northeastern of Thailand. The recipe was reported that claim to diabetic treatment. Aims: The studies were evaluated to hypoglycemic effect, serum insulin secretion and blood biochemistry in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: The recipe were composed of whole plants from A. indicum and M. Pudica (1:1 w/w) powder. The pound plants were macerated with aqueous (AMA), hydro-ethanol (AMHE) and 80\% ethanol (AME) to crude extracts. The AMA, AMHE and AME at the doses of 125, 250 and 500 mg/kg body weight (b.w.) were administered orally daily in diabetic rats during eight weeks. Fasting blood glucose levels (FBG) were measured at weekly. The serum insulin levels and blood biochemical data including blood urea nitrogen (BUN), creatinine (CREA), total protein (TP), albumin (Alb), serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), alkaline phosphatase (ALP), triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) were estimated at the end of experiment. Results: All doses of the extracts were showed significantly (p\<0.05) decreasing percent age of FBG in diabetic rats. Especially, AME 125 mg/ kg b.w. was showed more potent significantly (p\<0.05) decreasing percentage of FBG at week of 2, 5, 7 and 8. The serum insulin levels of all doses administered with the extracts were significantly (p\< 0.05) higher than diabetic control group. On the other hand, all doses of the extracts were significantly (p\< 0.05) decreasing ALT and ALP lower than diabetic control group. While, AMA and AMHE at the doses of 250 and 500 mg/kg b.w. were increased HDL, but decreased TC, TG and LDL. Conclusion: The study was proved to diabetic treatment and improvement of diabetic stage and blood biochemical parameters. In addition, the experiment was confirmed to folklore traditional use.

Key words: Thai folklore recipe, A. indicum , M. pudica , Fasting blood glucose.

}, keywords = {A. indicum, Fasting blood glucose., M. pudica, Thai folklore recipe}, doi = {10.5530/jppcm.2018.2s.xx}, author = {Ampa Konsue and Chusri Talubmook} } @article {584, title = {Effect of Thai Folklore Recipe from Abutilon indicum and Mimosa pudica in Streptozotocin-Induced Diabetic Rats}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {March 2018}, pages = {480-485}, type = {Original Article}, chapter = {480}, abstract = {

Context: Abutilon indicum and Mimosa pudica were a folklore recipe in Northeastern of Thailand. The recipe was reported that claim to diabetic treatment. Aims: The studies were evaluated to hypoglycemic effect, serum insulin secretion and blood biochemistry in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: The recipe were composed of whole plants from A. indicum and M. Pudica (1:1 w/w) powder. The pound plants were macerated with aqueous (AMA), hydro-ethanol (AMHE) and 80\% ethanol (AME) to crude extracts. The AMA, AMHE and AME at the doses of 125, 250 and 500 mg/kg body weight (b.w.) were administered orally daily in diabetic rats during eight weeks. Fasting blood glucose levels (FBG) were measured at weekly. The serum insulin levels and blood biochemical data including blood urea nitrogen (BUN), creatinine (CREA), total protein (TP), albumin (Alb), serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), alkaline phosphatase (ALP), triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) were estimated at the end of experiment. Results: All doses of the extracts were showed significantly (p\<0.05) decreasing percent age of FBG in diabetic rats. Especially, AME 125 mg/ kg b.w. was showed more potent significantly (p\<0.05) decreasing percentage of FBG at week of 2, 5, 7 and 8. The serum insulin levels of all doses administered with the extracts were significantly (p\< 0.05) higher than diabetic control group. On the other hand, all doses of the extracts were significantly (p\< 0.05) decreasing ALT and ALP lower than diabetic control group. While, AMA and AMHE at the doses of 250 and 500 mg/kg b.w. were increased HDL, but decreased TC, TG and LDL. Conclusion: The study was proved to diabetic treatment and improvement of diabetic stage and blood biochemical parameters. In addition, the experiment was confirmed to folklore traditional use.

}, keywords = {A. indicum, Fasting blood glucose, M. pudica, Thai folklore recipe}, doi = {10.5530/pj.2018.3.79}, url = {http://fulltxt.org/article/512}, author = {Ampa Konsue and Chusri Talubmook} } @article {333, title = {Anti-hyperglycemic and Anti-hyperlipidemic Effects of Extract from Houttuynia cordata Thumb. in Streptozotocin-Induced Diabetic Rats}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {April 2017 }, pages = {382-387}, type = {Original Article}, chapter = {382}, abstract = {

Aim: Various properties of Houttuynia cordata Thumb. has been reported. However, few studies on its pharmacological effects have been documented. To elucidate whether there are more pharmacological effects of this plant, this study was therefore, carried out to determine the anti-hyperglycemic and anti-hyperlipidemic effects of 80\% ethanol extract of H. cordata (HCE). Their antioxidant activity and acute toxicity were also conducted. Methods: HCE at a dose of 250 mg/kg was oral given to Streptozotocin-induced diabetic rats daily for 8 weeks. DPPH assay and HCE at the doses of 1,000, 2,000 and 3,000 mg/kg were employed in antioxidant and acute toxicity studies. Results: HCE lowered FBG in the diabetic, but not in the normal treated rats. HCE did not affect the body weight of all rats, but recovered TP, Alb, Glob, BUN, CREA, UA, TB, AST, ALT, ALP, and reduced the elevated CHO, TG and LDL in the diabetic rats. HCE possessed relatively low antioxidant activity with IC50 of 115.98\± 0.82 \μg/mL compared to Vitamin C (42.54+1.37 \μg/ml), but did not produce any symptoms of acute toxicity. Conclusions: The extract of H. cordata may have beneficial properties and is a new agent for diabetic treatment and improve renal and hepatic functions.

}, keywords = {Acute toxicity, Anti-Hyperglycemic, Anti-Hyperlipidemic, Antioxidant, Houttuynia cordata Thumb}, doi = {10.5530/pj.2017.3.65}, url = {/files/PJ-9-3/10.5530pj.2017.3.65}, author = {Patcharee Poolsil and Wilawan Promprom and Chusri Talubmook} } @article {334, title = {Antioxidant Activity, Biochemical Components and Sub-Chronic Toxicity of Different Brown Rice Kefir Powders}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {April 2017 }, pages = {388-394}, type = {Original Article}, chapter = {388}, abstract = {

Aims: This study aimed to investigate and compare antioxidant activity, biochemical components and subchronic toxicity of brown rice kefir powder from three different coloured rice varieties; Khao Dawk Mali 105 (KDMLKP, white), Red Hawm (RHKP, red), and Hawm Nil (HNKP, dark purple), with cow milk kefir powder (CMKP). Medthods: Antioxidant activity performed by using ferric reducing antioxidant power (FRAP) assay and 2, 2\´-diphenyl-1-picrylhydrazyl (DPPH) assay. Biochemical components values including gamma-aminobutyric acid and alpha-tocopherol were determined using high performance liquid chromatography, but total phenolic compounds was detemined using the Folin-Ciocalteu reagent. Sub-chronic toxicity test, KDMLKP, RHKP and HNKP at the doses of 150 mg/kg were given orally to the rats every day for 90 days. Results: Antioxidant activity showed that brown rice kefir powders were significantly (p\≤0.05) higher than cow milk kefir powder. Interestingly, HNKP provided the highest FRAB value and the lowest IC50 of DPPH assay. HNKP had also the highest biochemical component values. Repeat administration of KDMLKP, RHKP and HNKP did not produce any symptoms of sub-chronic toxicity; mortality, hematological values, and blood biochemistry in the rats. Moreover, RHKP produced HDL increasing without CHO, TG and LDL changing in rats. Conclusions: These data indicate that brown rice kefir powders show no sub-chronic toxicity and exhibit more antioxidant activity and biochemical component values than cow milk kefir powder. Surprisingly, RHKP showed HDL increasing while HNKP had the highest potent antioxidant activity and biochemical components. Thus, both rice kefir powders are safe and probably a new biochemical resource for health.

{\textquoteleft}}, keywords = {antioxidant activity, Biochemical Components, Brown Rice Kefir Powder, Rice, Sub-Chronic Toxicity}, doi = {10.5530/pj.2017.3.66}, url = {/files/PJ-9-3/10.5530pj.2017.3.66}, author = {Supaporn Chunchom and Chusri Talubmook and Sirirat Deeseenthum} } @article {323, title = {Chronic Toxicity of Leaf Extract from Sphagneticola trilobata (L.) Pruski}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {April 2017 }, pages = {323-328}, type = {Original Article}, chapter = {323}, abstract = {

Context: Sphagneticola trilobata (L.) Pruski. is a member of the family Asteraceae and has used traditionally in the prevention and treatment of various diseases. Aim: The research was aimed to determine chronic toxicity of 80\% ethanolic leaf extract from S.trilobata (STLE). Materials and Methods: STLE at the doses of 200 or 400 mg/kg b. w. was oral given to the healthy Wistar rats daily for 90 days. Statistical analysis used: Statistical analysis was carried out using F-test (One-Way ANOVA) followed by Duncan\’s New Multiple Range Test. Results: STLE did not produce any signs or symptoms of chronic toxicity. And also, the mortal rat was not observed during a period of an observation. Furthermore, STLE did not alter the body weight, relative organ (liver, pancreas, kidney and heart) weight, hemoglobin (Hb), hematocrit (Hct), red blood cell (RBC), white blood cell (WBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), neutrophil, lymphocyte, monocyte, platelet, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), creatinine, blood cell characteristics, ultrastructure of RBC, and histological features of hepatic, pancreatic and renal tissues in the STLE treated rats comparing to control rats. Conclusions: These findings indicate that the leaf extract from S. trilobata exerts non chronic toxicity in rats and can be used safely as a traditional medicine or diet complement without any effect on hepatic and renal functions.

}, keywords = {Blood biochemistry, Chronic toxicity, Hematological values, Histological feature, S. trilobata}, doi = {10.5530/pj.2017.3.55}, url = {/files/PJ-9-3/10.5530pj.2017.3.55}, author = {Areeya Suchantabud and Teeraporn Katisart and Chusri Talubmook} } @article {322, title = {Fasting Blood Glucose Levels and Hematological Values in Normal and Streptozotocin-Induced Diabetic Rats of Mimosa pudica L. Extracts}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {April 2017}, pages = {315-322}, type = {Original Article}, chapter = {315}, abstract = {

Context:M. pudica is a common plant found in moist waste ground, lawns, open plantations and weedy thickets. Aims: The fasting blood glucose levels (FBG) and hematological values of M. pudica aqueous(MPA) and hydro-ethanolic (MPHE) extract were evaluated in normal and streptozotocin (STZ)-induced diabetic rats. Materials and Methods: MPA and MPHE 125, 250 and 500 mg/kg body weight (b.w.) were administered orally and daily to the rats for 8 weeks. The FBG were determined weekly. Red blood cells (RBC), hemoglobin (HM. pudicab), hematocrit (Hct), platelet, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cells (WBC), lymphocytes, monocytes, neutrophils and eosinophil were evaluated. Results: MPA and MPHE had no effect on blood glucose levels in normal rats. All doses of all extracts showed significantly (p\<0.05) decreasing FBG in diabetic rats. Especially MPA at the dose of 250 mg/kg b.w. showed more potent significantly (p\<0.05) decreasing blood glucose levels than anti-diabetic drug glibenclamide at the end of experiment. All extracts had no effect on RBC, Hb, Hct, platelet, MCH, MCHC, lymphocytes, monocytes neutrophils and eosinophils. Surprisingly, the extracts were decreased WBC and MCV in diabetic rats. In addition, all of the extracts did not produce the alteration of blood cells structure in all rats. Conclusion: This study indicated that the extracts were hypoglycemic effect and improve hematological values in diabetes which confirms the traditional use of the plant.

}, keywords = {Blood glucose level, Hematological values, Mimosa pudica, Red blood cell, White blood cell}, doi = {10.5530/pj.2017.3.54}, url = {/files/PJ-9-3/10.5530pj.2017.3.54}, author = {Ampa Konsue and Chayan Picheansoonthon and Chusri Talubmook} } @article {227, title = {Toxicology and Oral glucose Tolerance Test (OGTT) of Thai Medicinal Plant Used for Diabetes controls, Phyllanthus acidus L. (EUPHORBIACEAE)}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {December 2016}, pages = {58-61}, type = {Original Article}, chapter = {58}, abstract = {

Aim: The present study aimed to investigate toxicity and oral glucose tolerance test (OGTT) of Phyllanthus acidus leaf extract (PAE) on Wistar rat. Methods: PAE was prepared and administered orally to experimental animals used. The extract was tested for toxicity in rats at a dose of 0, 1,000, 1,500 and 2,000 mg/kg body weight p.o once daily for 14 days. The hypoglycemic effects of PAE on normal rats and orally glucose-induced hyperglycemic rats were compared with distilled water and glibenclamide. A single dose (250 mg/kg body weight) of PAE was administered and blood glucose level was obtained by pricking the tail vain using glucometer at time -30, 0, 30, 60, 120 and 240 minutes. Results: All doses of the extract did not exert any sign or symptom of toxicity and the dead rat was not found. The body weight, white blood cell (WBC), mean corpuscular volume (MCV), platelet (PLT), plateletcrit (PCT), mean platelet volume (MPV), platelet distribution width (PDW), blood chemistry, blood urea nitrogen (BUN), creatinine, alkaling phosphatase (ALP) and organ weight of liver were not significantly different between control and treated rats. However, red blood cell (RBC), hematocrit (HCT), lymphocyte (LYM), and hemoglobin (Hb) at a dose 1,500 mg./kg body weight were significantly lower than those in the control group. The blood glucose levels of PAE treated groups were not different with control and Glybenclamide treated. Conclusion: The findings of the present study can be concluded that the PAE are practically non-toxic at a lower dose.

}, keywords = {Blood glucose, Diabetes, Medicinal plant, Phyllanthus acidus, Toxicity}, doi = {10.5530/pj.2017.1.11}, author = {Napapach Chaimum-aom and Sanong Chomko and Chusri Talubmook} }