@article {2369, title = {Apoptosis of The Lens Epithelial Cells After Ultraviolet-B Exposure as a Proposed Pathogenesis of Senile Cataract: Literature Review}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {August 2024}, pages = {989-992}, type = {Review Article}, chapter = {989}, abstract = {

Cataract shares a major burden causing half of the world{\textquoteright}s visual impairment and blindness. Ultraviolet light exposure has been associated with a higher incidence of cataracts. UV irradiation causes damage to the ocular tissue through protein crosslinking, enzyme dysfunction, ion pump inhibition, genetic mutation, and cell membrane disruption. Irreversible damage causes apoptosis to the lens epithelial cells, which is the main motor of lens homeostasis. Disturbance to the lens homeostasis will result in the loss of lens transparency and the development of cataracts. Understanding the pathophysiology of UV-induced cataract may bring to better approach to slower progression of the aging of the lens and formation of cataractous lenses.

}, keywords = {Nil}, doi = {10.5530/pj.2024.16.159}, author = {Ni Putu Ayu Reza Dhiyantari and Dicky Hermawan and Nurwasis} } @article {2340, title = {The Protective Effects of UV-blocking Spectacle Lenses and Class 1 UV-blocking Contact Lenses (Senofilcon A) on Superoxide Dismutase-2 and Catalase Expression in Lens Epithelial Cells Following UVB Radiation Exposure: An Experimental Study in Animal Model}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {August 2024}, pages = {785-790}, type = {Original Article}, chapter = {785}, abstract = {

Introduction: To determine SOD2 and CAT expression in lens epithelial cells with the protection of UVblocking spectacle lens and UV-blocking contact lenses after UVB radiation. Methods: 32 eyes of Rattus norvegicus mice were subjected to UVB radiation from Philips PL-S 9W/01 narrowband 311 nm lamps at a distance of 18 cm for 30 minutes. Divided into four groups: a control group, P1 group exposed to radiation, P2 group wearing UV-blocking spectacle lens, and P3 group wearing UV-blocking contact lens. The mice were euthanized three days later and had their eyes removed. Immunohistochemistry analysis was used to evaluate the expression of SOD2 and CAT. Results: P1 demonstrated a statistically significant SOD2 expression (p=0.002) compared to the control group. Neither P2 nor P3 exhibited significant differences in SOD2 expression (p=0.693; p=0.365). The SOD2 of the P1 group was significantly different compared to the P2 and P3 groups (p=0.007; p=0.023). No statistically significant difference between groups P2 and P3 (p=0.606). The CAT levels in the P1 group significantly differed from the control group (p=0.001). The differences between the P2 and P3 groups and the control groups were statistically insignificant (p=0.894; p=0.120). CAT P1 group had a significant difference with P2 and P3 groups (p=0.001; p=0.033). No statistically significant difference was observed between groups P2 and P3 (p=0.154). Conclusions: UV-B exposure decreases the expression of SOD2 and CAT. Both protection can provide equally good protection.

}, keywords = {CAT, epithelial lens, SOD2, UV-blocking contact lens., UV-blocking spectacles, UVB}, doi = {10.5530/pj.2024.16.130}, author = {Made Dessy Gangga Ayu Cinthiadewi and Nurwasis and Muhammad Firmansjah and Djoko Legowo and Ni Putu Ayu Reza Dhiyantari and Rifat Nurfahri} }