@article {2311, title = {Eosinophil Cell and Mass Appearance in Atypical Mycobacterium Infection of Lymphadenitis}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {June 2024}, pages = {664-667}, type = {Research Article}, chapter = {664}, abstract = {

Introduction: Atypical mycobacterium (ATM) is acid fast bacilli not including tuberculosis and may had opportunistic feature in environment either in air or soil. While symptoms of ATM infection may look similar with typical tuberculosis, these specific group of disease were harder to treat and would necessitate longer antibiotics consumption. While PCR may readily available to detect ATM, anatomical pathology method such as biopsy may be a cheaper alternative in low-resource settings to differentiate between atypical and typical mycobacterium infection. Aim: To analyze correlation between eosinophil cells and eosinophil mass with ATM in lymphadenopathy patient. Methods: This study is an analytical observational study with cross-sectional design which aimed to review diagnostic abilities of eosinophil cell and mass to detect ATM. Patient would undergo both PCR as gold standard of diagnosis and cytology biopsy aspiration as comparative diagnostic modalities. Data would be presented from SPSS v. 25. Results: We collected 70 subjects that fulfill inclusion and exclusion criteria. Most samples were dominated by female in relatively young age. There are 37 patients with ATM in which 75,7\% patients cytology result shown expression of eosinophil cells and 71,4\% patients shown eosinophilic mass. Chi-square test revealed that statistical significance existed between eosinophilic cells with ATM. However, such statistical significance was not found between eosinophilic mass and ATM. Conclusion: Eosinophilic cell can be used as alternative diagnostic modalities in diagnosing ATM. Further studies should further examine pathophysiological correlations and diagnostic power.

}, keywords = {Atypical mycobacterium, Cytology, Eosinophil}, doi = {10.5530/pj.2024.16.105}, author = {Neni Wahyu Hastuti and Delfitri Munir and Reno Keumalazia Kamarlis and Bintang Yinke Magdalena Sinag and Adang Bachtiar and Farhat and Amira Permatasari Tarigan and Delyuzar} } @article {2213, title = {Phytochemical Screening, Thin Layer Chromatography and Fourier Transform Infra-Red Spectroscopy Analysis of Eleutherine Bulbous (Mill.) Urb Bulb Extract}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {February 2024}, pages = {88-93}, type = {Original Article}, chapter = {88}, abstract = {

Background: Many traditional medicinal plants were studied for their phytochemical components. Dayak onions (Eleutherine bulbous (Mill.) Urb) is one of traditional medicinal plant in Indonesia. Analysis of the phytochemical compounds in the extract of the plant would help in determining various biological activities of the plant. Objective: This study was primarily aimed to identify the phytochemical profile of Dayak onions extract and its fractions. Methods: The Dayak onion bulbs were collected from Pancur Batu District, North Sumatra Province, Indonesia. Multiple solvents used to for extraction and analysis of phytochemical compound by chemical reactions, thin layer chromatography (TLC) profiling, and Fourier Transform Infrared Spectrophotometer (FTIR) profiling. Results: The phytochemical screening of Dayak onions ethanol extract showed it contained alkaloids, flavonoids, glycosides, tannins, and triterpenes/ steroids. The n-hexane fraction contained flavonoids and triterpenes/steroids, while the ethyl acetate fraction contains flavonoids and glycosides. The water fraction contained alkaloids, flavonoids, glycosides and tannins. By TLC profiling, the bioactive compounds in ethanol extract of the Dayak onion contain steroids, tannins, flavonoids and alkaloids. The bioactive compound in ethyl acetate fraction contains steroids, tannins, triterpenes, flavonoids and glycosides. The bioactive compound in n-hexan fraction contains steroids. The bioactive compound in water fraction fraction contains steroids and tannins. The FTIR demonstrated the presence of C-H alkanes, =C-H alkenes (aliphatic), C-O alcohol, ether, esters and carboxy acid in the ethanol extract, en-hexan fractions and ethyl acetate fractions. Conclusion: The phytochemical qualitative profiling showed that Dayak onion bulb as a rich source of bioactive compounds and have medicinal potential as therapeutic agent. A higher content of bioactive compounds of Dayak onion bulbs found in the ethanol and ethyl acetate extracts. The FTIR demonstrated the presence of C-H alkanes, =C-H alkenes (aliphatic), C-O alcohol, ether, esters and carboxy acid in the ethanol extract, enhexan fractions and ethyl acetate fractions.

}, keywords = {Dayak onion, Eleutherine bulbous, Extract, Fraction, FTIR, TLC}, doi = {10.5530/pj.2024.16.12}, author = {Lokot Donna Lubis and Muhammad Fidel Ganis Siregar and Farhat and Iqbal Pahlevi Adeputra Nasution and Dedy Syahrizal and Kamal Basri Siregar and Putri Chairani Eyanoer and Ichwanul Adenin and Muhammad Rusda and Mustafa M. Amin} }