@article {2226, title = {Effect of Lactiplantibacillus plantarum IS-10506 on Accelerating Repair of Ketorolac-Induced Gastric Ulcers in Wistar Rats}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {February 2024}, pages = {181-185}, type = {Original Article}, chapter = {181}, abstract = {

A gastric ulcer arises due to an imbalance between the stomach{\textquoteright}s aggressive and defensive factors, one of which can be induced by ketorolac. The gastric mucosa serves as a protective layer against gastric damage. Probiotics may enhance mucosal secretion, reinforcing their barrier function. This study evaluated the effect of Lactiplantibacillus plantarum IS-10506 on repairing gastric mucosal injury expressed by Mucin 5AC (MUC5AC) induction from ketorolac in rodent model. In the experiment, 48 male Wistar rats were randomly assigned and segregated into four groups: the control group (K1), the group with ketorolac administration (K2), the group given L. plantarum IS-10506 after ketorolac administration (K3), and the group given L. plantarum IS-10506 before and after ketorolac administration (K4). Gastric tissue was examined for cells producing MUC5AC via immunohistochemistry. MUC5AC differences between groups were compared using Kruskal{\textendash}Wallis and Mann{\textendash}Whitney U tests. Significant differences were observed between each group on Days 5, 7, and 10 of necropsy (p=0.043; p=0.030; p=0.022). The ketorolac group (K2) consistently exhibited the lowest values during all examination days. Group K4 manifested a higher expression of MUC5AC relative to group K3. However, group K3 demonstrated a significantly increased from Day 1 to 10 of necropsy (p=0.030). Administering probiotic L. plantarum IS-10506 prior to ketorolac proved beneficial by significantly accelerate (p=0.030) the MUC5AC expression cells and gene expression.

}, doi = {10.5530/pj.2024.16.25}, author = {Mia Ratwita Andarsini and Andy Darma and Alpha Fardah Athiyyah and Khadijah Rizky Sumitro and Reza Gunadi Ranuh and Fauziah Pratiwi and Wibi Riawan and Widjiati Widjiati and Ingrid S Surono and Subijanto Marto Sudarmo} } @article {2144, title = {Effect of Lactobacillus Plantarum IS-10506 on Paneth Cell Regeneration in the Ileum of Sprague Dawley Rats}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {October 2023}, pages = {928-932}, type = {Research Article}, chapter = {928}, abstract = {

Background: Pathogenic Escherichia coli (E. coli) is the most common infectious agent among children in developing countries. Indigenous probiotics are not widely used to treat diarrhea and intestinal infections. This study aims to investigate the cell regeneration process of paneth cells after administration of Lactobacillus plantarum IS-10506 due to damage caused by Lipopolysaccharide (LPS) E. coli O55:B5, through the expression of MATH-1 and DEFA-6. Methods: This study used 64 paraffin blocks from Rattus norvegicus strain Sprague-Dawley divided into four groups. There were three treatments, KN, KL, KP and KPR groups, The KN group represent the administration of placebo. The KL group received LPS E. coli O55:B5 on day one. The KP group received LPS E. coli O55:B5 on the first day and Lactobacillus plantarum IS-10506 on the second day until six-day. The KPR group were administered Lactobacillus plantarum IS- 10506 six days prior to receiving LPS E. coli O55:B5, respectively. All groups, except KN, received LPS at a dose of 250 μg/kg body weight once, and Lactobacillus plantarum IS-10506 at a dose of 2.86x1010 CFU/ day. Evaluating paneth cell regeneration, DEFA-6, and MATH-1 expression immunohistochemistry was conducted on all tissues. Results: The expression of DEFA-6 and MATH-1 in the KP and KPR groups on day three of observation was significantly higher from the KL group. Even though the KL group achieved significant growth, the results of this expansion were significantly smaller than KP and KPR groups. Conclusion: After mucosal injury caused by LPS E. coli O55:B5, administration of probiotic Lactobacillus plantarum IS-10506 may increase paneth cell regeneration through differentiation and cell number.

}, keywords = {DEFA-6, Escherichia coli O55:B5, Lactobacillus plantarum IS-10506, Lipopolysaccharide, MATH-1., Paneth cells}, doi = {10.5530/pj.2023.15.177}, author = {Rasio Putra Hutama and Alpha Fardah Athiyyah and I.G.M. Reza Gunadi Ranuh and Andy Darma and Khadijah Rizky Sumitro and Wibi Riawan and Ingrid S. Surono and Subijanto Marto Sudarmo} }