@article {2015, title = {Analysis of the Metabolite Compound of the Ethanol Extract of Chrysanthemum cinerariifolium Stem and Activity for inhibition of Oral Squamous Cell Carcinoma (OSCC) in silico study}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {April 2023}, pages = {393-398}, type = {Research Article}, chapter = {393}, abstract = {

Background: Oral cancer is a deadly disease that is expected to increase yearly. Current cancer treatment methods have side effects. C. cinerariifolium plants have potential as anticancer agents. Objective: To evaluate the anti-OSCC properties of the ethanol extract of C. cinerariifolium stems via an in-silico study. Materials and Methods: Analysis of active compounds in ethanol extracts of C. cinerariifolium stems using TLC and UPLC-QToF-MS/MS metabolic profiling. The data were analysed statistically using principal component analysis (PCA). In silico of C. cinerariifolium compounds on protein (PI3K and Cyclin D) from OSCC. Results: TLC procedures utilizing UV light with λ 366 nm after spraying with H2SO4 revealed multiple-colored spots, indicating that H2SO4 is a specific spray detector for terpenoid and carotene. Metabolic profiling in ethanol extract of C. cinerariifolium stem included Pronethalol (3.96\%), 1-(4-Methoxyphenyl)-N-(1 naphthylmethyl) methanamine (7.34\%), Orphenadrine (24.27\%), Pentazocine (5.09\%), 4-(Dodecyloxy) aniline (6.30\%), Linoleamide (4.95\%), and Pheophorbide A (8.05\%). Orphenadrine had the highest percentage. Based on the Lipinski rule of five, pronethalol has the potential to be used as a drug-like therapy for OSCC. The anticancer activity profile is predicted by PASS online with a likely range of 0.065 to 0.385. An in-silico study showed that the strongest binding affinity is pronethalol to Cyclin D1 and pheophorbide A to the PI3K protein. Conclusion: The active metabolite of the ethanolic extract of C. cinerariifolium stem exhibits potency against oral squamous cell carcinoma via the downregulation of the cell cycle (cyclin D1) and P13K, especially pronethalol.

}, keywords = {Chrysanthemum cinerariifolium, Cyclin D1, OSCC, P13K.}, doi = {10.5530/pj.2023.15.61}, author = {Anik Listiyana and Yuanita Lely Rachmawati and Hani Susianti and Nurdiana and Hidayat Sujuti and Roihatul Mutiah and Agustina Tri Endharti} } @article {1900, title = {Influence of Chitosan Concentration on Characteristic of Microspheres Delivery System Prepared from Eleutherine palmifolia (L.) Merr. Extract}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {December 2022}, pages = {702-709}, type = {Original Article}, chapter = {702}, abstract = {

Background: Eleutherine palmifolia (L.) Merr. (E.palmifolia) is a medicinal plant containing naphthoquinone, isoliquiritigenin, and oxyresveratrol compound. This study aims to formulate and characterize the E. palmifolia microspheres delivery system using chitosan variation as the polymer. Methods: The preparation method was the solvent evaporation method. The microspheres delivery system was performed by variation of chitosan concentration in each formulation 0.637\% (F1), 1.275\% (F2), and 1.912\% (F3). The physical and chemical characterizations include Entrapment Efficiency (EE), yield percentage, particle size, particle morphology, FTIR analysis, X-Ray Diffraction (XRD) analysis, and Differential Thermal Analysis (DTA) had been evaluated. Results: The results showed that chitosan concentration variation affected the microsphere{\textquoteright}s physical and chemical characteristics. Variations in the concentration of chitosan polymer (0.637\%, 1.275\% and 1.912\%) had an effect on the physical characteristics of the microspheres of the resulting Dayak onion bulb extract. The higher the concentration of chitosan used, the higher the entrapment efficiency and the yield yield will increase. Meanwhile, in observing the particle size, the higher the concentration of chitosan used, the smaller the particle size produced. The optimal concentration of chitosan as a polymer at concentration variations of 0.637\%, 1.275\% and 1.912\% to provide good physical characteristics of the Dayak onion bulb extract microspheres was shown in formula 3 with a chitosan concentration of 1.912\%. Conclusion: The better characteristic was obtained upon higher chitosan concentration. The best physical characteristics of E.palmifolia extract microspheres were obtained from chitosan concentration at 1.912\% (F3).

}, keywords = {Characteristic, Chitosan, Delivery system., Eleutherine palmifolia, Microspheres}, doi = {10.5530/pj.2022.14.157}, author = {Roihatul Mutiah and Wirda Ardania and Arief Suryadinata and Dewi Sinta Megawati and Anik Listiyana and Abdul Wafi and Rahmi Annisa} }