@article {2108, title = {Decreased Expression of Endocrine Glands Vascular Endothelial Growth Factor (EG-VEGF) in Rat Endometrial After Stimulation with Recombinants FSH Can be Reduce Implantation Rates}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {October 2023}, pages = {707-713}, type = {Original Article}, chapter = {707}, abstract = {

Background: Decreased expression of EG-VEGF in human endometrium after administration of ovarian stimulators has been reported to adversely affect endometrial receptivity and low implantation rates. Objective: To determine the effect of recombinant FSH administration on EG-VEGF protein in the endometrium of rats taken in the secretory phase and how it relates to endometrial receptivity. Design: A total of 36 female wistar rats with normal estrous cycles were randomly assigned to the natural cycle group (NC) and two stimulation groups (SC) which were injected with recombinant FSH at 12.5 IU and 25 IU intraperitoneally. Uterine necropsy and blood collection were performed on day 1, day 2, and day 3 after hCG administration. A total of 3 female rats from each group were mated with male rats (two males and three females in one cage). A successful marriage is indicated by the presence of a vaginal plug the next day. The level of EG-VEGF protein expression was assessed by immunohistochemical technique and steroid hormone levels were measured by the Elisa technique. Results: ANOVA test, that the expression of EG-VEGF in the endometrial glands showed a significant decrease from the normal cycle group to the stimulated cycle group 1 (SC 1) and SC2 (P = 0.00), as well as the expression of EG-VEGF in the endometrial stroma. (P = 000). Steroid hormone levels did not show a significant decrease between the normal cycle group and the stimulated cycle group (P = 0.48 and P = 0.13). Conclusion: Decreased EG-VEGF expression in rat endometrium after administration of recombinant FSH is associated with decreased endometrial receptivity which can reduce pregnancy rates.

}, keywords = {EG-VEGF, Endometrial receptivity, rFSH, Secretory phase.}, doi = {10.5530/pj.2023.15.141}, author = {Raden Muharam and Nurhuda Sahar and Kusmardi Kusmardi and Luluk Yunaini and Risqa Novita and Rosalina Thuffi and Conny Riana Tjampakasari and Ponco Birowo and Diyah Kristanty} } @article {1880, title = {Analysis Protein APOB and TroponinT in Obese Mice (Musmusculus) Induced by Static Magnetic Field as a Marker of Coronary Heart}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {October 2022}, pages = {627-631}, type = {Research Article}, chapter = {627}, abstract = {

Obesity is closely related to cardiovascular disease. APOB protein was a better predictor of LDL particle count. Increased troponin T protein is also indicated as an indication of the risk of CHD disease. Static Magnetic Field (SMF) is an electromagnetic applied in therapy because only a simple magnetic disk is used to generate it both in vitro and in vivo. The use of SMF is considered to affect the biological state of cells so that it can affect weight loss in mice. The method used was male c57bl/6j mice with groups of Obes0, Obes2, Obes7, Obes 14, and Obes21 fed high-fat diet and then exposed to SMF with an intensity of 2mT for 1 hour. The results obtained were that there was a significant difference p=0.015 (p\<0.05) in the protein concentration of APOB and there was no significant difference in the concentration of cTnT taken from the blood serum of mice. The increase in APOB protein levels in each control group was not found to have an increase in cTnT protein so that the two proteins did not have a linear correlation between the two proteins 0.598 (p\>0.05). Most of the protein concentrations of APOB and cTnT decreased in concentration at longer days. It can be seen morphologically in the heart there is a decrease in the number of adipose cells.

}, keywords = {Cardiovaskular, Electromagnetic, Obesity, Protein.}, doi = {10.5530/pj.2022.14.145}, author = {Puji Sari and Luluk Yunaini and Dwi Anita Suryandari and Widia Bela Oktaviani and Rahma Nur Istiqomah and Umiatin} }