@article {1807, title = {In Vivo Studies of Combined Probiotics on IFN-γ, Ig-E and Bronchial Muscular Layer of Rats with Allergic Asthma}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {June 2022}, pages = {598-603}, type = {Research Article}, chapter = {598}, abstract = {

Background: Leuconostoc mesenteriodes and Lactobacillus brevis are being used as probiotic to improve the immune system. The effect of probiotic containing combination of the 2 bacteria on enhancing innate and adaptive immune responses is not fully understood. Objective: This was conducted to analyse the effect of probiotic containing in single or combination content of L. mesenteriodes (Lm) and L. brevis (Lb) on IFN-γ concentration, Ig-E concentration, and histopathology of bronchial muscular layer of ovalbumininduced allergic asthma. Materials and Methods: A total of 40 male Sprague Dawley rats (6{\textendash}8 weeks, 200-300 g) were randomly divided into 5 groups: NC (non-induced control group); NgC (OVA-induced control group), Lm (OVA +probiotics containing L. mesenteriodes), Lb (OVA + probiotics containing L. brevis), and Lm + Lb (OVA+probiotics containing combination of L. mesenteriodes and L. brevis). On 64-day, concentration of IFN-γ and Ig-E in serum were measured. Histology of bronchus was performed. Results: IFN-γ concentration, Ig-E The administration of single or combined probiotics increased IFN-γ (p\<0.001), yet decreased IgE (p\<0.001) of all treatment groups, but only a combination of both probiotics reduced the thickness of the bronchial epithelium. Conclusion: The combination of Lm and Lb single or combined probiotics improve systemic and local anti-inflammation effects and ameliorate airway remodelling in the ovalbumin-induced chronic asthma rat model.

}, keywords = {Asthma, Bronchus histopatology, IFN-γ, Ig-E, Lactobacillus brevis, Leuconostoc mesenteriodes}, doi = {10.5530/pj.2022.14.77}, author = {Pujiati Pujiati and Soetrisno and Dono Indarto and Reviono} }