@article {2143, title = {Cardioprotective Effects of Thymoquinone on Myocardial Fibrosis}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {October 2023}, pages = {924-927}, type = {Research Article}, chapter = {924}, abstract = {

Introduction: Thymoquinone (TQ) is one of the active ingredients in herbal plants such as Nigella sativa which has antioxidant and anti-inflammatory properties thus may inhibits cardiac fibrosis formation. This study aims to determine the effectiveness of Thymoquinone as a cardioprotective agent in suppressing the extent of fibrosis in Wistar rats induced with lipopolysaccharide (LPS). Methods: This post-test only control study used 30 Wistar rats which were divided into 5 groups: saline, LPS-induced cardiac fibrosis, LPS-induced cardiac fibrosis treated with TQ 10 mg/mL, LPS-induced cardiac fibrosis treated with TQ 20 mg/mL, and LPS-induced cardiac fibrosis treated with TQ 40 mg/mL. Serum IL-6, GSH, and cTnT levels were measured using ELISA, and Mason{\textquoteright}s trichrome staining was used to assess myocardial fibrosis. Results: The LPS10+TQ20 and LPS10+TQ40 groups exhibited significantly lower levels of IL-6 compared to the LPS10+TQ10 group (p \< 0.05). GSH levels did not show a significant decrease in the TQ groups across different doses (p=0.771). The TQ-treated group demonstrated lower cTnT levels compared to the LPS-only group (p\<0.05). Thymoquinone treatment resulted in reduced fibrosis area compared to the LPS10 group (p\<0.05). Conclusions: TQ has a promising cardioprotective effect on the formation of cardiac fibrosis in Wistar rats induced with LPS.

}, keywords = {Cardiac Fibrosis, Lipopolysaccharide, Nigella sativa, Oxidative stress.}, doi = {10.5530/pj.2023.15.176}, author = {Saugi Abduh and Purwanto Bambang and Dirgahayu Paramasari and Soetrisno} } @article {1807, title = {In Vivo Studies of Combined Probiotics on IFN-γ, Ig-E and Bronchial Muscular Layer of Rats with Allergic Asthma}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {June 2022}, pages = {598-603}, type = {Research Article}, chapter = {598}, abstract = {

Background: Leuconostoc mesenteriodes and Lactobacillus brevis are being used as probiotic to improve the immune system. The effect of probiotic containing combination of the 2 bacteria on enhancing innate and adaptive immune responses is not fully understood. Objective: This was conducted to analyse the effect of probiotic containing in single or combination content of L. mesenteriodes (Lm) and L. brevis (Lb) on IFN-γ concentration, Ig-E concentration, and histopathology of bronchial muscular layer of ovalbumininduced allergic asthma. Materials and Methods: A total of 40 male Sprague Dawley rats (6{\textendash}8 weeks, 200-300 g) were randomly divided into 5 groups: NC (non-induced control group); NgC (OVA-induced control group), Lm (OVA +probiotics containing L. mesenteriodes), Lb (OVA + probiotics containing L. brevis), and Lm + Lb (OVA+probiotics containing combination of L. mesenteriodes and L. brevis). On 64-day, concentration of IFN-γ and Ig-E in serum were measured. Histology of bronchus was performed. Results: IFN-γ concentration, Ig-E The administration of single or combined probiotics increased IFN-γ (p\<0.001), yet decreased IgE (p\<0.001) of all treatment groups, but only a combination of both probiotics reduced the thickness of the bronchial epithelium. Conclusion: The combination of Lm and Lb single or combined probiotics improve systemic and local anti-inflammation effects and ameliorate airway remodelling in the ovalbumin-induced chronic asthma rat model.

}, keywords = {Asthma, Bronchus histopatology, IFN-γ, Ig-E, Lactobacillus brevis, Leuconostoc mesenteriodes}, doi = {10.5530/pj.2022.14.77}, author = {Pujiati Pujiati and Soetrisno and Dono Indarto and Reviono} }