@article {513, title = {GC-MS Analysis and Anti-Microbial Activities of Volatile Oil of Nardostachys Jatamansi D.C. Rhizomes Obtained from Haridwar Region, Uttrakhand}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {January 2018}, pages = {230-234}, type = {Original Article}, chapter = {230}, abstract = {

The Rhizomes of Nardostachys jatamansi D.C (Family Valerianceae) were collected from Haridwar, Uttarakhand. Aim: To identify the various volatile constituents of Nardostachys jatamansi D. C. Rhizomes and evaluation of antimicrobial activity. Method: The essential oil was collected by hydro distillation and analyzed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectroscopy (GC- MS). Volatile oil constituents, antimicrobial potential of Nardostachys jatamansi D. C. Rhizomes have been studied using different concentrations of the volatile oil against some micro-organisms. Results: A total of 10 compounds were identified from their fragmentation pattern. The Identified compounds were Calarene (20.4\%), Vardiflorene (12.3\%), \α- Panasinsen (9.7\%), \α- Santalene (4.6\%), \γ-Himachelene (17.1\%), Jatamansone (7\%), Ionol 4 (9.9\%), 2,2,7,7- Tetramethyl tricyclo[6,2,1,0 (1,6)] undec-4-ene 3-one (1.7\%), Epiglobulol (1.9\%) and Resibufogenin (8.4\%). Among them major constituents characterized was Calarene (20.4 \%) and followed by \γ-Himachelene (17.1\%) and Vardiflorene (12.3 \%). The volatile oil showed significant activity against all tested microorganisms. The maximum antibacterial activity was shown with 1\%v/v of volatile oil on Staphylococcus aureus (12.8 mm) followed by Escherichia coli (12.4 mm), whereas maximum anti-fungal activity was shown by 1\% v/v of volatile oil on Candida albicans (12.3 mm) followed by Aspergillus niger (11.9 mm). Conclusion: All identified constituents showed antibacterial activity and antifungal activity. Thus, the constituents should be further identified and analyzed by other spectrosopical techniques.

}, keywords = {Anti-microbial activity, GC GC-MS analysis, Hydro distillation, Nardostachys jatamansi DC, Volatile oil}, doi = {10.5530/pj.2018.2.40}, url = {http://fulltxt.org/article/470}, author = {Vijender Singh and Neha Rana and Mohd Ali} } @article {151, title = {Chemical Composition and Antimicrobial Activity of Fresh Rhizome Essential Oil of Zingiber Officinale Roscoe}, journal = {Pharmacognosy Journal,}, volume = {8}, year = {2016}, month = {February 2016}, pages = {185-190}, type = {Original Article}, chapter = {185}, abstract = {

Background: Zingiber officinale Roscoe (Zingiberaceae) is a rhizomatous perennial herb found in tropical Asia. It is extensively used worldwide as a spice, flavoring agent and herbal remedy for cold, throat and chest\ infections and cough. The present study was carried out to analyse an essential oil from the fresh rhizomes of Z. officinale of Ghaziabad region and to evaluate its antimicrobial activity. Materials and Methods: The fresh rhizomes were hydrodistilled to get the essential oil which was analysed by GC and GC-MS techniques. The oil was evaluated for antimicrobial activity by disc diffusion method. Results and Discussion: The essential oil was characterized by high percentage of sesquiterpenes (66.66\%), monoterpenes (17.28\%) and aliphatic compounds (13.58\%). The predominant sesquiterpene was zingiberene (46.71\%) followed by valencene (7.61\%), \β-funebrene (3.09\%) and selina-4(14),7(11)-diene (1.03\%). The major monoterpenes were characterized as citronellyl n-butyrate (19.34\%), \β-phellandrene (3.70\%), camphene (2.59\%) and \α-pinene (1.09\%). The essential oil exhibited significant antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. Conclusion: The essential oil mainly contained a large number of sesquiterpenes and monoterpenes and exhibited significant antimicrobial activity against pathogenic microorganisms.

}, keywords = {Antimicrobial activity, Chemical composition, Essential oil, GC-MS analysis, Zingiber officinale.}, doi = {10.5530/pj.2016.3.3}, author = {Pradeep Kumar Sharma and Vijender Singh and Mohammed Ali} }