@article {2016, title = {Preliminary Identification and Quantification of Quercetin Concentration and Its Comparison in Psidium Guajava L. (Guava) Fruit Ethanol Extract 50\% and 70\%}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {April 2023}, pages = {399-405}, type = {Research Article}, chapter = {399}, abstract = {

In 80 \% of developing countries as stated by official fact sheets and report a large proportion of the society still relies on traditional practitioners and their armamentarium of medicinal plants in order to meet health care needs. The study on Psidium Guajava L. fruit ethanol extract from Dukuhwaluh village, Purwokerto, Central Java, Indonesia showed its potential in increasing the number of megakaryocytes, followed by the rise of thrombocyte values. The study{\textquoteright}s objective was to compare the quantity of the quercetin content in 50\% and 70\% ethanol. This study was of experimental design and began by determining the effective concentration of two groups of guava fruits ethanol extract. The percentage of quercetin content dissolved within 50\% ethanol was 54.7344 mg/kg, and 70\% ethanol was 28.8420 mg/kg respectively. Quercetin content of Psidium Guajava L. fruit ethanol extract fruits in 50\% hydroethanolic was higher than that of 70\% hydroethanolic extract.

}, keywords = {Ethanol extract., Psidium Guajava L., Quercetin}, doi = {10.5530/pj.2023.15.62}, author = {Resti Gradia Dwiwina and Achadiyani and Dyah Dhianawaty and Irma Ruslina Defi and Nur Atik} } @article {1774, title = {Preliminary Identification and Quantification of Four Secondary Metabolites, Total Tannin and Total Flavonoid Contents in Guava Fruit Ethanol Extract}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {April 2022}, pages = {350-357}, type = {Research Article }, chapter = {350}, abstract = {

Introduction: The study on guava fruit ethanol extract from Dukuhwaluh Village, Purwokerto, Central Java, Indonesia showed increased megakaryocytes and platelet numbers in thrombocytopenic mice model. The study of acute oral toxicity of the extract did not show toxic effects in the kidney and liver at doses of 2000 and 5000 mg/kg b.w. The aim of the study was to determine the profile and quantity of four metabolite compounds and total tannin and flavonoid in the extract. Materials and Method: gallic acid, ellagic acid, rutin and kaemferol in the extract were identified and quantified by using high-performance liquid chromatography (HPLC) method with column LiChroCART 250-4,6 RP 18E, isocratic mobile phases with the composition of mixture:0.1\% acetic acid, acetonitrile and methanol (40:50:10) respectively, and at temperature 300C. The total tannin and flavonoid were determined using the by spectrophotometry method, Fe(III) chloride and 1.10-phenanthroline at wavelength 510 nm for tannin and aluminium chloride and rutin at wavelength 422 nm for flavonoid, respectively. Results: Percentage of gallic acid, ellagic acid, rutin and kaempferol were 0.77\%, 1.37 \%, 0.41 \% and 0.35 \%, respectively. Total tannin and flavonoid contents were 1.20\% (TAE) and, 1.18\% (RE) respectively. Conclusion: The guava fruit ethanol extract contained gallic acid, ellagic acid, rutin, kaempferol, tannin and flavonoid.

}, keywords = {Flavonoid, Guava fruit, Psidium guajava, Tannin}, doi = {10.5530/pj.2022.14.45}, author = {Diah Dhianawaty and Nur Atik and Resti Gradia Dwiwina and Iskandar Muda} }