@article {2164, title = {Comparison of the Anti-Inflammatory Activity of Flavonoid Bioactive Compounds Acetone Fraction and Steroid Fraction Ethyl Acetate Sungkai Leaves In Vivo and In Silico Studies}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {December 2023}, pages = {1068-1076}, type = {Original Article}, chapter = {1068}, abstract = {

One of the medicinal plants that is widely used by traditional communities is sungkai (Peronema canescens Jack), which is known to contain secondary metabolite compounds of flavonoids, saponins, tannins, steroids, terpenoids, alkaloids, and phenols. This research aims to isolate secondary metabolite compounds from acetone and ethyl acetate fractions, which act as anti-inflammatory agents in vivo and in silico. Acetone and ethyl acetate fractions were isolated and characterized using UV-Vis and FT-IR. The In Vivo test was carried out on Mus musculus, while the In Silico test was carried out using the IL-10 and IFN-γ receptors, which play a role in inflammation and immunity. Our research results showed that the ethanolic (F1) and n-hexane (F2) fractions of P. canescens extract had good anti-inflammatory activity with percent inhibition values of 58.12\% and 56.59\%, respectively. The characterization results showed that isolate F1 was a flavonoid group, Naringenin compound, while isolate F2 was a steroid group, β-Sitosterol compound. Moreover, from the scoring docking results, β-Sitesterol has tremendous potential as an anti-inflammatory than the compound naringenin.

}, keywords = {Anti-inflamatory; β-Sitesterol; Naringenin, P. canescens Jack}, doi = {10.5530/pj.2023.15.196}, author = {Madyawati Latief and Rahmani and Arliangga Fahrezi and Sutrisno and Indra Lasmana Tarigan} } @article {1772, title = {Determination Antioxidant Activity of Coffea Arabica, Coffea Canephora, Coffea Liberica and Sunscreens Cream Formulation for Sun Protection Factor (SPF)}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {April 2022}, pages = {335-342}, type = {Research Article }, chapter = {335}, abstract = {

Coffee is a highly consumed and popular beverage consumed in many countries. Its ingredients have a powerful antioxidant capacity and have the potential as sunscreen to protect the skin. This study aimed to examine the antioxidant activity of Arabica, robusta and liberica coffee extracts and the SPF activity of the coffee extract cream formulation. Coffee were macerated with three types of solvents with polarity gradients. The fraction of each coffee was analyzed for antioxidant activity using DPPH and formulated into the cream. Furthermore, the cream was analyzed for its SPF activity. The results of this study indicated that the methanol fraction of Robusta and Arabica coffee has the best antioxidant activity with IC50 values of 8.98 (ppm) and 13.13, respectively. Meanwhile, Liberica coffee has the best antioxidant in the ethyl acetate fraction, IC50 = 10.90 (ppm). In addition, the best SPF values for Robusta, Liberica and Arabica coffees were found in F3 cream with the category of Very Good Protection; 36.087 {\textpm} 0.0005; 35.007 {\textpm} 0.0005; 36,867 {\textpm} 0.0005 respectively.

}, keywords = {Antioxidant, Coffee, Cream, SPF}, doi = {10.5530/pj.2022.14.43}, author = {Madyawati Latief and Muhaimin and Heriyanti and Indra Lasmana Tarigan and Sutrisno} } @article {1905, title = {Isolation of a Flavone Apigenin and a Steroids Squalene from Peronema canescens Jack Leaves with Anti-Inflammatory Activities}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {December 2022}, pages = {744-752}, type = {Original Article}, chapter = {744}, abstract = {

The leaves of the P. canescens are ethnobotanically used by the community as a remedy for bruises and fever. This study aims to isolate both of ethanol and n-hexane fractions of P. canescens Jack leaves. Isolate then characterized and determined their anti-inflammatory activity with male white mice. Anti- Inflammatory was determined by the volume of exudate and \% inflammation inhibition. The isolates with good anti-inflammatory activity were characterized using UV-Vis and IR spectrophotometry. Our findings showed that the ethanolic (F1) and n-hexane (F2) fractions of P. canescens extract had a good anti-inflammatory activity with percent inhibition values of 58.12\% and 56.59\%, respectively. The characterization results showed that isolate F1 was a flavonoid group, Apigenin compound, while isolate F2 was a steroid group, Squalene compound.

}, keywords = {Anti-inflammatory., Apigenin, P. canescens, Squalene}, doi = {10.5530/pj.2022.14.162}, author = {Indra Lasmana Tarigan and Sutrisno and Rumaida and Indah Pramana Sari Aini and Madyawati Latief} } @article {1782, title = {Preliminary Data on the Antibacterial Activity of Coffea Arabica, Coffea Canephora and Coffea Liberica}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {April 2022}, pages = {413-424}, type = {Research Article}, chapter = {413}, abstract = {

The continuous use of synthetic antibiotics will not only kill bacteria but also can accelerate the emergence of resistant pathogenic races. In addition, it can also cause the death of normal flora. Coffee extract is reported to have the potential as a naturally active and non-toxic antibacterial compound suitable for biomedical applications. Extraction using methanol was carried out on green coffee and which had been roasted for three types of Jambi coffee, namely Arabica, Robusta and Liberica coffee and continued with fractionation using hexane and ethyl acetate. The results of the analysis using FTIR showed that OH, C-H, C = C, C = O, C-O, C-N and N-H groups were detected. These functional groups are probably the functional groups that belong to caffeine, trigonelline, nicotinic acid and dehydrocafestol, which are believed to have bacteriostatic effects on some bacteria. The SEM-EDX analysis results of the three types of coffee showed that the dominant elements were O, K and Mg. Potassium and magnesium minerals bind with chlorogenic acid to form salt complexes of chlorogenic acid and magnesium chlorogenic acid complex. The antibacterial activity of the coffee extract and fraction was still low with the diameter of the inhibition zone was still low (0-10 mm). Furthermore, further characterization and tests are needed to confirm the antibiotic potency of the Arabica, Robusta and Liberica coffee ethanol extracts.

}, keywords = {Antibiotics, Coffee, E. coli}, doi = {10.5530/pj.2022.14.53}, author = {Madyawati Latief and Heriyanti and Indra Lasmana Tarigan and Sutrisno} }