@article {1957, title = {Evaluation of Antidiarrheal, Antispasmodic, and Antisecretory Activities of Extract and Fractions of Castanopsis costata Leaves in Animal Models}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {March 2023}, pages = {31-37}, type = {Original Article }, chapter = {31}, abstract = {

Background: The use of traditional medicinal plants Castanopsis costata in the management of diarrhea is an ancient practice in North Sumatra, Indonesia, but its safety and efficacy have not been examined scientifically in animal models. Therefore, this study aims to determine the antidiarrheal effect of C. costata leaf extract and fractions in vivo. Methods: The antidiarrheal activity was evaluated against animal models of diarrhea induced by castor oil, charcoal meal, and entero-pooling test. The extract group received doses of 25, 50, 100, and 200 mg/kg, while the fraction groups was treated with WFCC, EAFCC, and nHFCC, in doses of 50 and 100 mg/kg, respectively. The negative control received 10 mL/kg of the dissolving vehicle, while the positive was treated with loperamide 3 mg/kg. Results: The results showed that EECC doses of 200 mg/kg, WFCC, and EAFCC at 50 and 100 mg/kg, respectively significantly delayed the onset of diarrhea, while WFCC and EAFCC at 100 mg/kg significantly reduced the amount of wet feces output and total feces output. In addition, only WFCC and EAFCC (at 50 and 100 mg/kg, respectively) significantly reduced the mean weight of wet feces and total feces. The percentage of maximum diarrhea inhibition was observed at the EAFCC dose of 100 mg/kg. Based on the charcoal meal test results, only EAFCC significantly inhibited the distance traveled by charcoal meal and reduced the peristaltic index at a dose of 100 mg/kg, while the percentage decrease in gastrointestinal motility was 46.87\%. Similarly, in the entero-pooling test, WFCC and EAFCC significantly reduced the volume and weight of intestinal contents at a dose of 100 mg/kg, respectively. Conclusion: The results confirm that the extract and fractions of C. costata have antidiarrheal activity. Therefore, this study provides scientific support for the traditional use of C. costata in treating diarrhea.

}, keywords = {Animal models, Castanopsis costata, Diarrhea, North Sumatra., Traditional medicine}, doi = {10.5530/pj.2023.15.5}, author = {Maulana Yusuf Alkandahri and Mally Ghinan Sholih and Nitya Nurul Fadilah and Maya Arfania and Surya Amal and Dedy Frianto and Lina Aliyani Mardiana and Diany Astuti and Dadang Muhammad Hasyim} } @article {1716, title = {Antidiabetic Activity of Extract and Fractions of Castanopsis costata Leaves on Alloxan-induced Diabetic Mice}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {December 2021}, pages = {1589-1593}, type = {Original Article}, chapter = {1589}, abstract = {

Background: In North Sumatra, Castanopsis costata is commonly used by traditional practitioners for the treatment of diabetes mellitus, however, no studies have been carried out to substantiate this practice. Therefore, this study aims to evaluate the anti-diabetic activity of extract and fractions of C. costata leaves in mice with alloxan-induced diabetes. Methods: Swiss albino mice with alloxan-induced diabetes, were acquired and subjected to the experimental evaluations. Subsequently, the fasting blood glucose levels of the diabetic mice groups treated with glibenclamid, extract and fractions, as well as the untreated group, were evaluated after two weeks of therapy. Results: Based on the results, the ethanolic extract of C. costata considerably reduced the mice{\textquoteright}s blood glucose levels in a dose-dependent manner, at dosages of 25, 50, 100, and 200 mg/kgBW (\% DBGL: 21.10\%, 46.36\%, 58.94\%, and 60.93\%, respectively). In addition, the water fraction of C. costata leaves produced a greater reduction in blood glucose levels (\% DBGL: 78.93\%), compared to the ethyl acetate and n-hexane fractions (\% DBGL: 67.06\% and 58.83\%), respectively. Meanwhile, treatment with the antidiabetic drug, glibenclamide (5 mg/kgBW) produced a 50.75\% reduction in blood glucose levels. Conclusion: Based on the findings, the extract and fractions of C. costata leaves were concluded to exhibit significant anti-diabetic activity. This supported the claim that traditional practitioners in North Sumatra use the plant extract for diabetes treatment.

}, keywords = {Antidiabetic activity, Castanopsis costata, Diabetes mellitus, North Sumatra.}, doi = {10.5530/pj.2021.13.204}, author = {Maulana Yusuf Alkandahri and Dani Sujana and Dadang Muhammad Hasyim and Mareetha Zahra Shafirany and Lela Sulastri and Maya Arfania and Dedy Frianto and Farhamzah and Anggun Hari Kusumawati and Nia Yuniarsih} }