@article {1865, title = {Potential Role of Mitragynine as Lipolysis Stimulator via Adrenergic Signalling: Docking Model Study}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {October 2022}, pages = {527-531}, type = {Original Article}, chapter = {527}, abstract = {

Backgrounds: Mitragynine is the most popular of the more than 50 alkaloids contained in M.Speciosa. In particular, the Mitragynine alkaloid has the potential to increase lipid (fats) metabolism through specific pathways such as adenylyl cyclase signaling via adrenergic receptors. In this case, Asp Amino acid and Ser are the types of residues that can activate adenylyl cyclase to initiate a series of activities in cells. Methods: This study used Mitragynine ligand and adrenergic receptors (α1b, α2a, α2b, α2c dan β1). The receptor candidates were tested using Autodock whose test results were presented in the form of tables and 3-dimensional images using the Biovia Discovery Studio. Results: Hydrogen bonds were formed between Mitragynine and the amino acids Asp and Ser at the β1-adrenergic receptor. The binding amino acids were found in Ser20 and Asp21 with energy bond of -5.26 kcal/mol and IC50: 111.35 ppm. Meanwhile, at the adrenergic receptor α2b there was only Asp residue that formed hydrogen bond with Mitragynine namely Asp218A. The energy bond formed between the two was -5.19 kcal/mol and IC50: 125.04 ppm. Conclusion: Mitragynine has the potential to stimulate lipolysis through the pathways of α2b and β1-adrenergic receptors.

}, keywords = {Adrenergic, Docking, Lipolysis, Mitragynine}, doi = {10.5530/pj.2022.14.130}, author = {Khoirul Rista Abidin and Ronny Lesmana and Mas Rizky Anggun Adipurna Syamsunarno and Kelana Kusuma Dharma} } @article {1652, title = {Separation and Quantification of Sinensetin, Imperatorin and Total Tannin Content as Active Phytoconstituents of Methanol Extract of Imperata cylindrica Root from Kendari}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {September 2021}, pages = {1216-1224}, type = {Research Article}, chapter = {1216}, abstract = {

Introduction: Imperata cylindrica root methanol extract harvested from Kendari had anti-hypertensive activity stated in a study on hypertension rat models at the doses 60 and 90 mg/kg-bw. The extract also contained phenolic compound 1.53\% (GAE) and flavonoid 1.17\% (QE) and had antioxidant activity IC50 0.32 mg/mL. Aim of the study was to determine the active secondary metabolites in the extract. Materials and Method: Sinensetin and imperatorin in the extract were separated on silica gel 60 column, eluted with a gradient eluent system as follows (1) n-hexane- ethyl acetate (3:7); (2) n-hexane {\textendash} ethyl acetate (2:8); (3) ethyl acetate -MeOH (8:2), and (4) methanol. Imperatorin and sinensetin in fractions were identified and quantified by HPLC method with column LiChroCART 250-4,6 RP 18E, isocratic mobile phases with the composition of mixture as follows, 0.1\% formic acid, acetonitrile and methanol (40:50:10) respectively, and at temperature 400 C. The total tannin was determined by spectrophotometry method with Fe(III) chloride and 1.10-Phenanthroline at wave length 510 nm. Results: Imperatorin and sinensetin were identified in fractions 5 to 9. The concentrations of sinensetin and imperatorin in Imperata cylindrica root methanol extract were of 32.348 and 37.014 mg/kg extract, respectively. The total tannin content was 1.06 \% (w/w) (TAE). Conclusion: Imperata cylindrica root methanol extract from Kendari had active phytoconstituents, namely sinensetin, imperatorin, and tannin.

}, keywords = {Imperata cylindrica, Imperatorin, Sinensetin, Tannin}, doi = {10.5530/pj.2021.13.155}, author = {Diah Dhianawaty and Mas Rizky Anggun Adipurna Syamsunarno and Resti Gradia Dwiwina and Raden Anita Indriyanti and Ruslin} }