@article {1646, title = {Evaluation of the Anticonvulsant, Anxiolytic, Sedative, and Neuroprotective Activities of Polysaccharides from Mycelium of Two Ganoderma Species}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {September 2021}, pages = {1161-1173}, type = {Research Article}, chapter = {1161}, abstract = {

Background: Ganoderma lucidum has been used as a medicinal mushroom since centuries in East Asia. Recent reports have shown that metabolites isolated from Ganoderma species have shown effects on central nervous system. Objective:\ To determine the neuroprotective, anticonvulsant, anxiolytic, and sedative effects of Ganoderma sp. and Ganoderma curtisii polysaccharides. Methods: Polysaccharides (Gsp-PS2 or Gc-PS2) were isolated from two Ganoderma mycelia submerged cultures. Acute toxicity effects of Gc-PS2 or Gsp-PS2 on mice were treated orally with doses of 50 - 2000 mg/kg. Anticonvulsant activity was determined using three chemoconvulsants: kainic acid (KA), strychnine, or pentylenetetrazole (PTZ). Anxiolytic-like effects were determined using the elevated plus maze test on mice. GABA release evoked by GC-PS2 or Gsp-PS2 content was determined by HPLC. Neuroprotective effects of Gsp-PS2 or Gc-PS2 were determined by glial activation, histopathological changes, and immunohistochemistry. Results: Gc-PS2 or Gsp-PS2 showed neuroprotective activity by diminishing neuronal death, reducing glial activation and Neu-N expression levels. Gsp-PS2 or Gc-PS2 inhibited convulsions in the KA model. An anxiolytic-like, but not a sedative effect was reported in mice treated with Gc-PS2 or Gsp-PS2. Polysaccharides Gc-PS2 or Gsp-PS2 evoked endogenous GABA release and increased its concentration within the incubation medium. Pretreatment with Gsp-PS2 or Gc-PS2 showed a reduction of the LPSinduced NO production. Gc-PS2 or Gsp-PS2 did not produce toxic effects. Conclusion:\ Ganoderma sp. or Ganoderma curtisii polysaccharides showed neuroprotective and anticonvulsant activities in animal models. The anticonvulsant activity may involve the GABAergic neurotransmision.

}, keywords = {a- and b-glucan, Anticonvulsant, GABA, Ganoderma curtissi, Ganoderma sp, Neuroprotective}, doi = {10.5530/pj.2021.13.149}, author = {Veronica Nunez-Urquiza and Juana Villeda-Hernandez and Elizur Montiel-Arcos and Isaac Tello and Victoria Campos-Pena and Maribel Herrera-Ruiz and Mar{\'\i}a del Carmen Guti{\'e}rrez and Vera Petricevich and Mar{\'\i}a Ang{\'e}lica Santana and Martha Navarro and Ang{\'e}lica Berenice Aguilar-Guadarrama and Gabriel Navarrete-V{\'a}zquez and Irene Perea-Arango and Ismael Leon-Rivera} }