@article {1695, title = {In vitro Wound Healing Potential and Antimicrobial Activity of Clerodendrum Inerme Leave Extracts}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1542-1548}, type = {Review Article}, chapter = {1542}, abstract = {

Background: Clerodendrum inerme is a medicinal plant which exhibited many pharmacological effects. However, wound healing property of this plant has not been investigated. Objective: The present study was designed to evaluate wound healing and antimicrobial activities of C. inerme leaves using the in vitro model with Human keratinocytes (HaCaT). Methods: Cytotoxicity and wound healing effects were determined by MTT and scratch wound healing assay. Antimicrobial activities against cutaneous flora and clinical isolates of bacteria were investigated by broth microdilution assay. Results: The results showed that the water extract of C. inerme leaves did not exhibit cytotoxic effects on HaCaT cells, while ethanol extracts at higher concentrations significantly decreased cell growth with an IC50 value of 386.8 {\textpm} 87.1 μg/mL. The water extract and the lowest concentration (6.25 μg/mL) of the ethanol extract significantly increased percentage of wound closure compared with the untreated group. The water and ethanol extracts of C. inerme displayed a broad spectrum of antibacterial activity, inhibiting growth of Staphylococcus aureus, Staphylococcus.epidermidis, Escherichia coli, and Pseudomonas aeruginosa. The water extract displayed remarkable activity against methicillin-resistant S. aureus with MIC and MBC values ranging from 0.39 to 1.56 μg/mL. Notably, it provided stronger antibacterial activity than vancomycin and also showed antifungal activity against C. albicans. Conclusion: This study confirms the potential of C. inerme leaves for wound healing and antimicrobial therapy and supports the continued utilization of C. inerme leaves in traditional medicine. Further studies are needed to clarify the molecular mechanisms through which it exerts such biological effects.

}, keywords = {Antimicrobial activity, Clerodendrum inerme, Keratinocytes, Scratch assay, Wound Healing}, doi = {10.5530/pj.2021.13.196}, author = {Sueptrakool Wisessombat and Malatee Tayeh} } @article {1223, title = {Anti-migration and Anti-invasion Abilities of Methanolic Leaves Extract of Clerodendrum Inerme on Lung Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {August 2020}, pages = {1024-1031}, type = {Original Article}, chapter = {1024}, abstract = {

Background: Clerodendrum inerme is a folk medicinal plant used in the treatment of various illnesses such as a cough, scrofulous infection, venereal infection, skin diseases and tumors. It exhibited many pharmacological effects including hepatoprotective, anti-inflammatory, antibacterial, anti-oxidant and anticancer properties. Objective: The purpose of this study was to investigate the influence of methanolic extract of C. inerme leaves on migration, invasion and adhesion activities on human lung adenocarcinoma. Materials and methods: Cytotoxicity, cell motility, migration, invasion and adhesion abilities were detected by MTT, wound healing, trans-well mobilization, modified Boyden chamber and cell adhesion assay, respectively. Results: The results demonstrated that up to 400 μg/mL methanolic leaves extract has low toxicity on A549 cells showing more than 50\% cell viability. At non-cytotoxic and sub-toxic doses (200 and 400 μg/mL) of methanolic leave extract significantly suppressed cell motility, migration, invasion and cell adhesion compared with the untreated control. Conclusion: These results suggested that methanolic leaves extract of C. inerme inhibited migration, invasion and adhesion of A549 cells. These findings showed new therapeutic potential for C. inerme in anti-metastatic therapy.

}, keywords = {Anti-invasion, Anti-migration, Clerodendrum inerme, Human lung adenocarcinoma}, doi = {10.5530/pj.2020.12.145}, author = {Malatee Tayeh and Poonsit Hiransai and Hathaichanok Kommen and Ramida Watanapokasin} }