@article {1461, title = {Phytochemical and analytical evaluation of Cordia dichotoma Linn. leaves}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {27th Nov, 2014}, pages = {58-63}, type = {Original Article}, chapter = {58}, abstract = {

Background: An ethnomedicinally important plant, Cordia dichotoma Linn is practiced in various indigenous systems of medicine and popular among the various ethnic groups in India for the cure of variety of ailments as an astringent, anthelmentic, diuretic, demulcent, anti-diabetic and expectorant. Because of the increasing demand, maintaining quality standards is the need of the day. Aims and Objectives: The present study was designed to set standard pharmacognostical, physicochemical, phytochemical, fluorescence and HPTLC chromatographic profile of the leaves of Cordia dichotoma Linn (CD). Materials and Methods: CD, which was previously authenticated, was subjected to pharmacognostical, physicochemical, fluorescence and high performance thin-layer chromatography (HPTLC) analysis as per standard protocol. Results and Conclusion: The final observations were recorded. The loss on drying at 105\ºC was found to be 8.5\% w/w, total ash value 13\% w/w, acid-insoluble ash 5.07\% w/w, water-soluble ash 5.49\% w/w, water-soluble extractive 9.2\% w/w, alcohol-soluble extractive 5.81\% w/w and pH (1\% aqueous extract) 6.88. Phytochemical screening showed the presence of steroid, carbohydrate, alkaloid, saponin, cardiac glycosides, flavonoid and phenolic compounds in methanolic extract. The CD fluorescence was seen in UV light and it was of different colour in different solvents. HPTLC analysis revealed 5 peaks at wavelength 366 nm with max Rf values in the range of 0.3 to 0.93. The purity and quality of the leaves of Cordia dichotoma or pharmaceutical preparations prepared from it can be tested by pharmacognostical, physicochemical, fluorescence and HPTLC observations of the present study..

Key words: Cordia dichotoma, Fluorescence analysis, Physicochemical parameters, HPTLC chromatogram.

}, keywords = {Cordia dichotoma, Fluorescence analysis, HPTLC chromatogram., Physicochemical parameters}, author = {Md Azizur Rahman and Arshad Hussain} } @article {37, title = {Phytochemical and analytical evaluation of Cordia dichotoma Linn. leaves}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {01/2015}, pages = {58-63}, type = {Original Article}, chapter = {58}, abstract = {

Background: An ethnomedicinally important plant, Cordia dichotoma Linn is practiced in various indigenous systems of medicine and popular among the various ethnic groups in India for the cure of variety of ailments as an astringent, anthelmentic, diuretic, demulcent, anti-diabetic and expectorant. Because of the increasing demand, maintaining quality standards is the need of the day. Aims and Objectives: The present study was designed to set standard pharmacognostical, physicochemical, phytochemical, fluorescence and HPTLC chromatographic profile of the leaves of Cordia dichotoma Linn (CD). Materials and Methods: CD, which was previously authenticated, was subjected to pharmacognostical, physicochemical, fluorescence and high performance thin-layer chromatography (HPTLC) analysis as per standard protocol. Results and Conclusion: The final observations were recorded. The loss on drying at 105\ºC was found to be 8.5\% w/w, total ash value 13\% w/w, acid-insoluble ash 5.07\% w/w, water-soluble ash 5.49\% w/w, water-soluble extractive 9.2\% w/w, alcohol-soluble extractive 5.81\% w/w and pH (1\% aqueous extract) 6.88. Phytochemical screening showed the presence of steroid, carbohydrate, alkaloid, saponin, cardiac glycosides, flavonoid and phenolic compounds in methanolic extract. The CD fluorescence was seen in UV light and it was of different colour in different solvents. HPTLC analysis revealed 5 peaks at wavelength 366 nm with max Rf values in the range of 0.3 to 0.93. The purity and quality of the leaves of Cordia dichotoma or pharmaceutical preparations prepared from it can be tested by pharmacognostical, physicochemical, fluorescence and HPTLC observations of the present study.

}, keywords = {Cordia dichotoma, Fluorescence analysis, HPTLC chromatogram., Physicochemical parameters}, doi = {10.5530/pj.2015.7.7}, author = {Md. Azizur Rahman and Arshad Hussain} } @article {1500, title = {Antiproliferative activity of crude extract and fractions obtained from Digera muricata on Hela cell lines of human cervix and A549 cell lines of Human Lung.}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {3rd Sept, 2014}, pages = {32-38}, type = {Original Article}, chapter = {32}, abstract = {

Digera muricata (Linn.) of family Amaranthaceae is an ethanobotanically important plant species traditionally used against various disorders. Cytotoxic potential of methanolic extract and its fractions were investigated against Hela and A549 cell lines. Crude extract of Digera muricata was prepared in methanol by Continuous Hot Soxhlation technique. Crude extract was fractionated into two organic and one aqueous fraction by the help of Column Chromatography. MTT assay was used to evaluate the reduction of viability of the cancer cell lines. Cell viability was inhibited by crude extract of Digera muricata in a dose dependent manner ranging from 25\μg/ml to 250\μg/ml. Apoptosis assays using nucleic acid stains namely PI exclusion assay and Hoestch/PI assay were performed by the help of fluorescence microscopy. Morphological analysis was done by calculation of Apoptotic ratio and Percentage apoptosis. Our results suggests that methanolic and aqueous fraction of the extract of Digera muricata can be good source of cytotoxic compounds.

Key words: 3-(4,5-dimethylthiazol-2yl)-2,4 diphenyltetrazolium bromide assay, A549 cell line, cytotoxic, Digera muricata, HeLa cell line.

}, keywords = {3-(4, 4 diphenyltetrazolium bromide assay, 5-dimethylthiazol-2yl)-2, A549 Cell Line, Cytotoxic, Digera muricata, HeLa cell line}, author = {Shazia Usmani and Arshad Hussain and A.H.A Farooqui and Mohd.Arshad and Sahabjada Siddiqui and Mohd.Ahmad and Shadma Wahab} }