@article {1420, title = {Both Ethanol and Ethyl Acetate Curcuma Zedoaraia Extract was Capable of Inducing Cells Death in T47D Cell Line Culture}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {May 2021}, pages = {737-743}, type = {Research Article}, chapter = {737}, abstract = {

Introduction: Curcuma zedoaria (CZ) has been proven capable of inducing apoptosis in cells cancer. CZ extraction can be performed by ethanol and acetyl acetate as solvent. However, which one of these extracts is superior remains unclear. Objective: This study aimed to investigate the difference potential effect of ethanol and acetyl CZ extract on apoptosis of T47D cell line. Methods: In this study 21 wells were assign into seven groups: control group (T47D); treatment groups consisting of group of ethanol CZ extract 46 (EtZ-46); group of ethanol CZ extract 23 (EtZ-23); group of ethanol CZ extract 11 (EtZ-11); and group of ethyl acetate CZ extract 111 (AcZ-111); group of ethyl acetate CZ extract 55 (AcZ-55); and group of ethyl acetate CZ extract 27 (AcZ-27). In T47D group only loaded with T47D cell line; in treatment groups aside from loaded with T47D cell line culture, also treated with ethanol or acetyl acetate CZ extract respectively. Concentration of T47D cell was 5 x 104 T47D cells line in 100 μl suspension loaded on each well of 21 wells and kept in CO2 incubator overnight. The apoptosis cells were measured after 48 hours post CZ treatment. Results: Post Hoc analysis indicated that the number of apoptosis cells in AcZ-111 was significant higher compared to that of other groups, p\<0.05. Conclusion: Acetyl acetate CZ extract treatment with dose 111 μg was capable of inducing apoptosis in T47D cell line superior than that of other groups including ethanol CZ extract.

}, keywords = {Acetyl acetate, Apoptosis, Curcuma zedoaria, Ethanol, Necrosis, T47D cell line}, doi = {10.5530/pj.2021.13.94}, author = {Titiek Sumarawati and Chodidjah and Taufiqurrachman Nasihun} } @article {1139, title = {Pimpinella Treatment on Reducing Apoptosis of Kidney Cells Following UVB Radiation in Rats}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {503-509 }, type = {Original Article}, chapter = {503}, abstract = {

Introduction: Pimpinella alpina Molk (PM) is a botanical antioxidant was able to inhibit apoptosis in various cells. Apoptosis is a leading cause of tubular atrophy and therefore chronic kidney disease. However, the effect of PM on reducing apoptosis in kidney cells remains unclear. Objective: aim of this study to elucidate the effect of PM on reducing apoptosis in kidney cells. Methods: In the post test only control group design, 35 male rats were grouped into 7 comprise: NC-G, samples were neither exposure to UVB nor PM treatment; NG-7 and NG-15, all samples were only exposure to UVB irradiation for 7 days; P10-7, P15-7, P10-15, P15-15 groups, samples were exposure to UVB for 7 days and treated with PM for 7 and 15 days respectively. Bax and Caspase3 expression were assessed by rt-PCR and IHC staining method. Results: Statistical analysis showed that RNA-Bax and RNA-caspase3, Bax and caspase3 protein expression in P15-7, P10-15 and P15-15 were lower significantly compared to those of NG-7, p\<0.05, and no significant difference compared to those of NC-G, p \> 0.05. Conclusion: PM treatment with 100 and 150 mg/day for seven and fifteen days were able to decrease Bax and Caspase3 expression in kidney cells following UVB irradiation. Even, the decreased in Bax and caspase3 expression were comparable to normal.

}, keywords = {Apoptosis, Bax, Caspase3, Kidney Cells, Pimpinella alpina Molk}, doi = {10.5530/pj.2020.12.77 }, author = {Taufiqurrachman Nasihun and Eni Widayati} }