@article {1152, title = {Development and Validation of Stability Indicating HPLC Method for Determination of Caffeic Acid, Vitexin and Rosmarinic Acid in Thunbergia laurifolia Leaf Extract}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {611-618}, type = {Research Article}, chapter = {611}, abstract = {

Thunbergia laurifolia has been a popular herb used in Thai traditional medicine for detoxification and as antipyretic. It contains rosmarinic acid (RA), caffeic acid (CA) and vitexin as major compounds. In order to control the herbal quality, the stability indicating high-performance liquid chromatography (HPLC) was developed and validated. The stability study of compounds in T. laurifolia leaf extract was investigated. The chromatographic separation was performed using a reversed-phase C18 column and mobile phase consisted of 0.5\% acetic acid and methanol using a gradient elution with 1.0 mL/min flow rate. The detection wavelength was set at 330 nm. The method was validated for its linearity, precision, accuracy, limit of detection and limit of quantitation. Forced degradation of three compounds in extract showed that they were stable in oxidative condition, but highly labile under alkaline hydrolytic conditions. All three compounds in T. laurifolia leaf extract were stable at room temperature at least 3 months while a remarkable decrease of RA, vitexin and CA in the extract were found in accelerated condition. This finding could be applied for predicting the storage recommendation and expiry of T. laurifolia extract and its related pharmaceutical products.

}, keywords = {Force degradation, Phenolic compound, Phytochemical screening, Stabilityindicating method}, doi = {10.5530/pj.2020.12.91 }, author = {Nanthakarn Woottisin and Sumet Kongkiatpaiboon and Sophida Sukprasert and Korbtham Sathirakul} } @article {1117, title = {Phytochemical Compounds in Arundo donax L. Rhizome and Antimicrobial Activities}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {287-292}, type = {Original Article}, chapter = {287}, abstract = {

Introduction: The aerial part of Arundo donax L., giant reed, is a well-known fuel source used in many countries. Methods: Phytochemical compounds in A. donax L. rhizome, sequentially extracted with hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and methanol (MeOH), were identified using gas chromatography-mass spectrometry. Antimicrobial activities of the rhizome extracts were evaluated using disc diffusion assay against yeast (Candida albicans), and bacteria Gram-positive (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC11778, and Bacillus subtilis ATCC6633) and Gram-negative (Escherichia coli ATCC25922). Results: The detected phytochemicals were screened against WILEY07 library; 84 compounds matched with a similarity >= 90\%. All the characterized compounds were grouped based on their functional group. The major phytochemicals in the HEX, DCM, and EA extracts belonged to sterol groups, while lipids, fatty acids, and related conjugates were the main components of the methanolic extract. The other characterized compounds were hydrocarbons, phenolics, terpenoids, xanthones, and xanthene. Growth of B. subtilis was inhibited by the HEX, DCM, EA, and MeOH extracts, whereas B. cereus growth was inhibited only by the DCM and EA extracts. However, growth of E. coli and C. albicans could be not inhibited by A. donax L. rhizome extracts. Analysis of the compounds as well as their antibacterial activities via hierarchical clustering showed that hexadecanoic acid is the major compound influencing B. subtilis growth, while, B. cereus growth was affected by xanthone. Conclusions: Rhizomes of A. donax L. is one potential source of antimicrobial agents and further applied in medicinal uses.

}, keywords = {Bacteria, GC-MS, Giant reed, HCA}, doi = {10.5530/pj.2020.12.45}, author = {Kanoktip Pansuksan and Sophida Sukprasert and Netiya Karaket} }