@article {1729, title = {Phytochemistry, Antioxidant Activity and Cytotoxicity Evaluation of Black-White Fungus Auricularia sp. against Breast MCF-7 Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {February 2022}, pages = {01-07}, type = {Original Article}, chapter = {01}, abstract = {

Introduction: Cancer is a non-communicable disease with over 18.1 million new cases and 9.6 million deaths annually according to WHO. Breast cancer is the number two highest count type of cancer trailing behind lung cancer. Treating cancer is expensive and have various side effects. Active components found in plants or fungi that have antioxidant and cytotoxic activity towards cancer cells, could be an alternative for anticancer. One of the fungi that is potentially developed as an anticancer, are the genus of Auricularia sp. also known as black-white fungus. This study aims to determine the phytochemicals components, antioxidant activity and cytotoxic effect of the Auricularia sp. towards MCF-7 breast cancer cells. Methods: Dried black-white fungus of Auricularia sp. grinded into a fine powder. Then, multilevel maceration is done with the n-hexane, ethyl acetate, ethanol as solvents. The extracts of black-white fungus undergo phytochemical screening and thin layer chromatography (TLC), followed by measuring antioxidant and evaluating the cytotoxic activity towards MCF-7 breast cancer cells. Results: black-white fungus of Auricularia sp. contained secondary metabolites of flavonoids, alkaloids, and triterpenoids and a total of 17 other phytochemical components. Ethyl acetate extract of black-white fungus showed a weak antioxidant activity towards DPPH free radical with IC50 of 215.51 μg/mL and a very active cytotoxic activity on MCF-7 cells with IC50 of 0.21 μg/mL. On the other hand, ethanol and n-hexane extracts of black-white fungus are categorized with an active cytotoxic activity on MCF-7 cells with IC50 of 29.28 μg/mL and 50.39 μg/mL, respectively. Conclusion: Black-white fungus Auricularia sp. that had anticancer activity towards breast MCF-7 cells should be considered as an alternative treatment for breast cancer therapy.

Key words: Phytochemistry, Antioxidant, Cytotoxicity, Black-white fungus Auricularia sp., MCF-7 cells.

}, doi = {10.5530/pj.2022.14.1}, author = {Ade Arsianti and Arfi Rabbani and Anton Bahtiar and Norma Nur Azizah and Lince Dameria Nadapdap and Ajeng Megawati Fajrin} } @article {1091, title = {Phytochemical Composition and Evaluation of Marine Algal Sargassum polycystum for Antioxidant Activity and In Vitro Cytotoxicity on Hela Cells}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {February 2020}, pages = {88-94}, type = {Original Article}, chapter = {88}, abstract = {

Introduction: Sargassum polycystum is one of marine algal which has a potent antioxidant anticancer activities. This research aims to investigate phytochemical composition, antioxidant activity and in vitro cytotoxicity of marine algal Sargassum polycystum on cervical HeLa cancer. Methods: Sargassum polycystum collected from Dompu beach, Lombok, Nusa Tenggara Barat Province, Indonesia, were extracted into organic solvent of n-hexane, ethylacetate, chloroform and ethanol, respectively. Subsequently, Sargassum polycystum extracts were applied for Thin Layer Chromatography (TLC) analysis, phytochemistry test, total phenolic and total flavonoid contents, as well as for antioxidant activity test by DPPH (2,2-diphenyl-1-picrylhydrazyl) method, and in vitro cytotoxicity evaluation on HeLa cells by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide) assay. Results: Phytochemical analysis of S. polycystum extracts are positive for metabolites of flavonoid, steroid, tannin and glycoside. TLC analysis revealed that S. polycystum extracts containing four phytochemical components. Ethylacetate extract of S. polycystum showed the highest total phenolic content, and exhibited greater antioxidant activity than ethanol extract. Total phenolic and total flavonoid content in ethylacetate extract are 548.61 μg/mL and 40.06 μg /mL, respectively. Ethylacetate extract of S. polycystum with IC50 value of 298.3 μg/mL is assigned to have a weak antioxidant activity against DPPH free radical. The results indicate that antioxidant activity of ethylacetate extracts of S. polycystum is directly correlated with its total phenolic and flavonoid content. Moreover, S. polycystum extracts demonstrated a strong anticancer activity on cervical HeLa cells with IC50 ranging from 38.3 μg/mL to 112.8 μg/mL. Conclusion: This work confirmed that S.polycystum are promising natural antioxidant and anti-cervical cancer agents.

}, keywords = {Antioxidant, Cytotoxicity, HeLa cells, phytochemisty, Sargassum polycystum}, doi = {10.5530/pj.2020.12.14}, author = {Ade Arsianti and Anton Bahtiar and Vincent Kharisma Wangsaputra and Norma Nur Azizah and Wilzar Fachri and Lince Dameria Nadapdap and Ajeng Megawati Fajrin and Hiroki Tanimoto and Kiyomi Kakiuchi} } @article {1116, title = {Phytochemical Profile, Antioxidant Activity and Cell Line Study of Marine Red Macroalgae Eucheuma cottonii on Lung A-549 Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {276-281}, type = {Original Article}, chapter = {276}, abstract = {

Introduction: In this century, cancer has increased in incidence and become one of the deadliest disease in the world. However, to date, lung cancer treatments are still not fully effective, quite expensive and very exhaustive for the patient. Eucheuma cottonii is an abundant marine red macroalgae in Indonesia which have a potential anti-lung cancer properties. Aim of this research is to determine phytochemical profile of Eucheuma cottonii extracts, as well as to evaluate its antioxidant and cytotoxic effects on Lung A-549 cancer cells. Methods: Eucheuma cottonii obtained from Sorong beach, West Papua Province, Indonesia, were extracted with three different solvents, that is ethanol, ethylacetate, and n-hexane. These three Eucheuma cottonii extracts were identified for its phytochemical profiles, antioxidant activity by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, and cytotoxic activity on lung A-549 cells by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Results: Phytochemical analysis revealed that Eucheuma cottonii contains metabolites of triterpenoid and alkaloid. Antioxidant activity evaluation showed ethanol extract of Eucheuma cottonii has IC50 value of 559.76 μg/mL against DPPH free radical. Whereas cytotoxicity evaluation showed that ethanol extract and ethylacetate extract of Eucheuma cottonii have cytotoxic effects on Lung A-549 cancer cells, with IC50 value of 251.73 μg/ mL and 261.41 μg/mL, respectively. Conclusion: These results suggesting that Eucheuma cottonii extract could be further developed as a natural anti-lung cancer agent.

}, keywords = {Antioxidant, Cell line study, Eucheuma cottonii, Lung A-549 cells, Phytochemical}, doi = {10.5530/pj.2020.12.43}, author = {Ade Arsianti and Gerry Kurniawan and Nadzila Anindya Tejaputri and Fona Qorina and Qotrunnada Fithrotunnisa and Norma Nur Azizah and Ajeng Megawati Fajrin} } @article {1108, title = {Synthesis, Characterization, and Cytotoxicity Evaluation of Gallic Acid Nanoparticles Towards Breast T47D Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {321-327}, type = {Original Article}, chapter = {321}, abstract = {

Introduction: Gallic acid is a naturally polyphenolic acid which shows cytotoxicity against several cancer cells, as well as it displays chemo-preventive activity which is attributed to its strong apoptosis- inducing and antioxidant effects. Thus, gallic acid has become an attractive substance to be further developed due to its strong cytotoxic activity. This study aimed to synthesize gallic acid nanoparticle coating with alginate-chitosan, and evaluate its cytotoxicity against breast T47D cancer cells. Methods: Gallic acid nanoparticle was synthesized using ionic gelation method. The yield, size and morphology of the nanoparticles were determined by UV-Vis Spectroscopy, Transmission electron microscopy (TEM) and Fourier Transform Infrared (FTIR) spectroscopy. Cytotoxicity evaluation of gallic acid nanoparticle towards breast T47D cancer cell is carried out by MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide) assay. Results: Spherical nanoparticles of gallic acid with the size of 100-200 nm has been successfully synthesized in 96\% of yield. Compared to gallic acid (IC50: 20.86 μg/mL) and alginate-chitosan nanoparticle (IC50: 38.46 μg/mL), gallic acid coating with alginate-chitosan nanoparticles demonstrated higher cytotoxicity towards breast T47D cancer cells with IC50 value of 9.03μg/mL. Conclusion: Our results clearly confirmed that gallic acid nanoparticles coating with alginate-chitosan showed a strong cytotoxicity towards breast T47D cancer cells, which is potential to be developed as a candidate for new anti-breast cancer agent.

}, keywords = {Cytotoxicity, Gallic acid, Nanoparticle, Synthesis, T47D cells}, doi = {10.5530/pj.2020.12.51}, author = {Ade Arsianti and Anton Bahtiar and Fadilah Fadilah and Vincent Kharisma Wangsaputra and Rafika Indah Paramita and Norma Nur Azizah and Lince Dameria Nadapdap and Ajeng Megawati Fajrin and Hiroki Tanimoto and Kiyomi Kakiuchi} }