@article {1760, title = {The Activities of Pegagan Embun (Hydrocotyle sibthorpioides Lam.) on TNF-α, Macrophages and Leukocytes Male White Mice Exposed by H5N1 Virus Antigens}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {April 2022}, pages = {253-261}, type = {Original Article}, chapter = {253}, abstract = {

Introduction: Pegagan embun (Hydrocotyle sibthorpioides Lam.) has been known to have immunostimulatory activity, it can increase the activity and capacity of mice macrophage phagocytosis at optimum dose of 200 mg/kgbw. Aim: This study aims to determine the activity of the ethanol extract of pegagan embun on TNF-α levels, total macrophages, total leukocytes, and percentage of leukocytes types. Methods: Mice were divided into four groups, one group as control was given 0.5\% Na CMC suspension, three groups were given pegagan embun extract at a dose of 10 mg/kgbw, 50 mg/kgbw and 200 mg/kgbw orally for 7 days. On the 8th day, all mice were induced with 0.3 mL H5N1 vaccine subcutaneously, then left for 24 hours. After 24 hours, the mice were sacrificed and then TNF-α levels, total macrophages, total leukocytes, and percentage of leukocytes types were determined. The research data were analyzed using one-way ANOVA (significance was taken at p\<0.05) and continued with the Duncan test. Results: The results showed that pegagan embun extract significantly reduce TNF-α levels and total macrophages, also significantly increase total leukocytes and percentage of leukocyte types in mice (p\<0.05). Significant increases and decreases occurred on average at doses of 50 mg/kgbw and 200 mg/kgbw. Conclusion: Based on these results, it can be concluded that pegagan embun extract have immunomodulatory activity.

}, keywords = {Hydrocotyle sibthorpioides Lam., Immunomodulators, Leukocytes, Leukocytes types, Macrophages, TNF-α}, doi = {10.5530/pj.2022.14.31}, author = {Salman Umar and Nengsyih Putri Erman and Armenia and Elsa Badriyya and Yufri Aldi} } @article {1329, title = {The Impact of Sub Acute Administration of Purified Gambier (Uncaria gambir Roxb.) to The Liver and Kidney Functions and its Reversibility on Rats}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {January 2021}, pages = {44-51}, type = {Original Article}, chapter = {44}, abstract = {

Introduction: The impact of sub-acute administration of purified gambier (Uncaria gambir Roxb.) to the liver and kidney function and its reversibility had been studied on rats. Methods: Rats at the aged of 2-3 months and the bodyweight of {\textpm}250 g were treated with water solution of purified gambier at the dose of 5 mg/kg10 and 20 mg/kg for 7 to 14 consecutive days. Plasma ALP, AST activities, creatinine clearance, liver and kidney ratios were determined on the day 1, 7, 14 one week after the doses stopped. All data on each parameter were analyzed using two-way ANOVA followed by Duncan{\textquoteright}s multiple T-test and significance was taken at p\<0.05. Results: The results showed that all parameters was not affected significantly (p\>0.1), except ALT activity and liver organ ratio decreased significantly (p\<0.05). Conclusion: These indicated that purified gambier is relatively non-toxic to the liver and the kidney of the rats at doses of 5-20 mg/kg BW for 14 days.

}, keywords = {ALP, ALT, CrCl, Liver function, Purified gambier, Renal function}, doi = {10.5530/pj.2021.13.7}, author = {Armenia and Dita Permatasari and Lathifah Putri Sinamar and Keke Estera and Almahdy Ahmadin} } @article {1069, title = {Subacute Toxicity of Butanol Fraction of Tali Putri Plants (Cassytha filiformis L.) Against Hematology Parameters of White Male Mice}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {February 2020}, pages = {25-28}, type = {Original Article}, chapter = {25}, abstract = {

Hematotoxicity study of butanol fraction of Cassytha filiformis L. on white male mice has been performed. A total of 12 white male mice were used, and they were divided into four groups. Each group consists of 3 mice. These animals were treated with Cassytha filiformis L. butanolic fraction at several doses of 2.5, 5, and 10 mg/kg for seven days. Hemoglobin value, erythrocytes, platelets, leukocytes, monocytes, lymphocytes, eosinophils, neutrophils, and basophils counts were measured at the 1st, 3rd, and 7th day of treatment. The data of this study were analyzed using two-way ANOVA, followed by Duncan{\textquoteright}s multiple region tests. The results showed that the doses of butanol fraction did not affect hemoglobin value, erythrocytes, leukocytes, and neutrophils (p \>0.1), but it significantly reduced platelet, monocyte, lymphocyte count, and increases eosinophils (p \<0.01) counts within their normal limits. There is no influence on the duration of administration and the interaction of dosage, and also the duration of use of the parameters above. This result implies that the Cassytha filiformis butanol fraction at doses of 2.5-10 mg/kg does not affect hematology parameters if it were used for seven days.

}, keywords = {Butanol fraction, Cassytha filiformis L, Haematology, Subacute toxicity}, doi = {10.5530/pj.2020.12.5}, author = {Dwisari Dillasamola and Annisa Yatursyi and Armenia} }