@article {1067, title = {In vitro Cytotoxicity of Hibiscus sabdariffa Linn Extracts on A549 Lung Cancer Cell Line}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {February 2020}, pages = {14-19}, type = {Original Article}, chapter = {14}, abstract = {

Background: Lung cancer is the one of the leading causes of cancer death. However, current treatments for lung cancer are expensive and show negative side effects. Therefore, the study concerning natural anticancer from plants has intensified. Hibiscus sabdariffa Linn are Indonesian herb plants which have been consumed as a drink, are known to have anticancer activity against several cancer cell lines. However, its potential cytotoxic activity on A549 lung cancer cell line is still unclear. Objective: This study aimed to identify cytotoxic activity of Hibiscus sabdariffa Linn extracts on A549 lung cancer cell line. Materials and Methods: Hibiscus sabdariffa Linn flowers from Tangerang, province of Banten, Indonesia, were macerated in three different solvents: ethyl acetate, ethanol, and n-hexane. Afterwards, cytotoxic activity of Hibiscus sabdariffa Linn extracts on A549 lung cancer cell line were evaluated using MTT assay. There were eight variety of concentration of the extracts, the experiment has been done triplicate for each concentration. The anticancer activity is expressed by IC50 value. Results: Hibiscus sabdariffa Linn extracts in ethanol, ethyl acetate, and n-hexane showed IC50 value of 374.01 μg/mL, 719.28 μg/mL, and 906.57 μg/mL respectively, in which indicated weak cytotoxic activity on A549 lung cancer cell line. Conclusion: Ethanol, ethyl acetate, and n-hexane extracts of Hibiscus sabdariffa Linn are potential to be further developed as natural anticancer agents.

}, keywords = {A549 Lung Cancer Cell Line, Cytotoxicity, Hibiscus sabdariffa Linn}, doi = {10.5530/pj.2020.12.3}, author = {Qotrunnada Fithrotunnisa and Ade Arsianti and Gerry Kurniawan and Fona Qorina and Nadzila Anindya Tejaputri and Norma Nur Azizah} } @article {1116, title = {Phytochemical Profile, Antioxidant Activity and Cell Line Study of Marine Red Macroalgae Eucheuma cottonii on Lung A-549 Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {276-281}, type = {Original Article}, chapter = {276}, abstract = {

Introduction: In this century, cancer has increased in incidence and become one of the deadliest disease in the world. However, to date, lung cancer treatments are still not fully effective, quite expensive and very exhaustive for the patient. Eucheuma cottonii is an abundant marine red macroalgae in Indonesia which have a potential anti-lung cancer properties. Aim of this research is to determine phytochemical profile of Eucheuma cottonii extracts, as well as to evaluate its antioxidant and cytotoxic effects on Lung A-549 cancer cells. Methods: Eucheuma cottonii obtained from Sorong beach, West Papua Province, Indonesia, were extracted with three different solvents, that is ethanol, ethylacetate, and n-hexane. These three Eucheuma cottonii extracts were identified for its phytochemical profiles, antioxidant activity by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, and cytotoxic activity on lung A-549 cells by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Results: Phytochemical analysis revealed that Eucheuma cottonii contains metabolites of triterpenoid and alkaloid. Antioxidant activity evaluation showed ethanol extract of Eucheuma cottonii has IC50 value of 559.76 μg/mL against DPPH free radical. Whereas cytotoxicity evaluation showed that ethanol extract and ethylacetate extract of Eucheuma cottonii have cytotoxic effects on Lung A-549 cancer cells, with IC50 value of 251.73 μg/ mL and 261.41 μg/mL, respectively. Conclusion: These results suggesting that Eucheuma cottonii extract could be further developed as a natural anti-lung cancer agent.

}, keywords = {Antioxidant, Cell line study, Eucheuma cottonii, Lung A-549 cells, Phytochemical}, doi = {10.5530/pj.2020.12.43}, author = {Ade Arsianti and Gerry Kurniawan and Nadzila Anindya Tejaputri and Fona Qorina and Qotrunnada Fithrotunnisa and Norma Nur Azizah and Ajeng Megawati Fajrin} }