@article {962, title = {A New LC/MS/MS Method for the Analysis of Phyllanthin in Rat Plasma and its Application on Comparative Bioavailability of Phyllanthin in Different Formulations after Oral Administration in Rats}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {September 2019}, pages = {968-975}, type = {Original Article}, chapter = {968}, abstract = {

Introduction: A simple, short UPLC/MS/MS method for quantitation of phyllanthin in rat plasma in less than 2 minutes have been developed and fully validated. The validated method was used to investigate the pharmacokinetic properties of phyllanthin in PA extract and phospholipid complex of PA extract in rat. Methods: The separation was carried out on Acquity C18 (50 x 2.1 mm; 1.7 μm), with a mobile phase of 10 mM aqueous amonium acetate and acetonitrile (10:90; v/v), at a flow rate of 0.2 mL/min. Felodipin was used as internal standard. Phyllanthin is extracted from a small volume of rat plasma (100 μl) by means of liquid-liquid extraction method with tert butyl methyl ether. Electrospray ionization (ESI) mass spectrometry was applied in positive mode at capillary voltage of 4000 V for both phyllanthin and IS, cone voltage of 24 V for phyllanthin and 20 V for IS, desolvation temperature of 360oC, cone gas flow of 25 L/h, collision energy of 12 V for phyllanthin and 10 V for IS. Multiple reaction monitoring (MRM) was used to monitor the transitions at m/z (Q1/Q3) 436.41/355.36 for phyllanthin and 384.20/352.18 for IS. Results: The linear calibration curve of phyllanthin was obtained over the concentration range of 0.5 {\textendash} 100 ng/mL. The intra- and inter-day precisions were less than 7.08 \% and the accuracies were within {\textpm} 7.55\%. The Cmax values of phyllanthin from two different preparations in rat plasma after oral administration of 2.0 mg/kg were 11.44 and 31.44 ng/ml, and the AUC values were 18.07 and 41.43 h.ng/ml, respectively. Conclusion: A simple, short UPLC/MS/MS method for quantitation of phyllanthin in rat plasma in less than 2 minutes have been developed and fully validated. The bioavailability of phyllanthin from the phospholipid complex of PA extract in rat plasma was significantly improved compared with that of raw PA extract after oral administration.

}, keywords = {LC-MS/MS, Pharmacokinetics, Phospholipid, Phyllanthin, Plasma, Quantitation}, doi = {10.5530/pj.2019.11.153}, author = {Nguyen Van Long and Chu Van Men and Anh Vu Tuan and Nguyen Van Manh and Thanh Chu Duc and Ha Bui Thi Thu and Hoang Van Luong and Le Bach Quang and Pham Gia Khanh} } @article {987, title = {Phytosomal Nanoparticles Preparation of Curcuminoids to Enhance Cellular Uptake of Curcuminoids on Breast Cancer Cell Line MCF-7}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {September 2019}, pages = {1037-1045}, type = {Original Article}, chapter = {1037}, abstract = {

Objective: Curcuminoids, the bioactive compounds extracted from Curcuma longa consisting of Curcumin (CUR), demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC), have shown promising biological effects, including anticancer activity. This study sought to prepare a physically stable phytosomal nanoparticles of curcuminoids (Curs-Phyto) to facilitate uptake of curcuminoids on breast cancer cells line, and further increase the cytotoxicity against cancer cells. Methods: The evaporation combined extrusion technique was employed to prepare phytosomal curcuminoids nanoparticles. The interaction between curcuminoids and phospholipid by a hydrogen bond was confirmed by differential scanning calorimetry (DSC), powder X-ray diffraction (XRD), fourier transform infrared (FT-IR), and 1H nuclear magnetic resonance (1H-NMR). Their physicochemical characterizations and stability in simulated gastric and intestinal media were investigated. The effects of Curs-Phyto on MCF-7 cells were evaluated by flow cytometry, MTS assay and cell cycle analysis. Results: We found that the Curs-Phyto were formed at a spherical shape with good size (~ 180 nm), a narrow size distribution (PDI \< d0.2), high complexation rate (~ 87\%, 95\%, and 90\% for BDMC, DMC, and CUR respectively) and high loading capacity of curcuminoids. More importantly, the Curs-Phyto showed the increased cellular uptake and enhanced cytotoxicity against MCF- 7 cancer cells, compared to free curcuminoids. Conclusion: These results indicated that the phytosome could be a promising oral delivery system for curcuminoids for cancer treatment.

}, keywords = {Cellular uptake, Curcuminoids, FACS analysis, Nanoparticle, Phytosome}, doi = {10.5530/pj.2019.11.163}, author = {Nguyen Van Long and Bui Thi Thu Ha and Anh Vu Tuan and Hoang Van Luong and Nguyen Tung Linh and Thanh Chu Duc and Phung Cao Dai and Chul Soon Yong and Chu Van Men} }