@article {1155, title = {Investigation of Secondary Metabolites and its Bioactivity from Sarocladium kiliense SDA20 Using Shrimp Shell Wastes}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {636-644 }, type = {Research Article}, chapter = {636}, abstract = {

Introduction: In this study we isolated bioactive compounds using different chromatographic techniques from culture filtrate of Sarocladium kiliense SDA20 grown in fermentation media containing shrimp shell waste as substrate under optimum conditions. Antibacterial and antibiofilm activities of crude extract and purified compounds were evaluated. Methods: The test fungi strain Sarocladium kiliense SDA20 was isolated from Egyptian soil and identified by18 S ribosomal RNA. Optimization conditions were carried out in fermentation media containing shrimp shell waste as sole carbon source, inoculated by 106 spores/ml of Sarocladium kiliense SDA20 at pH 7.0 produce 84.5\% of the total toxins. Different chromatographic techniques for ethyl acetate extract of culture filtrate of fungi were used resulting in isolation of pure compounds were elucidated spectroscopically and comparing their data in literature. GC/MS analysis of extract was used for identification of other chemical compounds. Antibacterial and biofilm activity was evaluated using MTT assay. Results: Five compounds for the first time were identified: Cholest-5-en-3-ol (C1), Palmitic acid (C2), Oleic acid (C3), Nicotinamide (C4), Tricin (C5). GC-MS analysis showed the presence of twenty-seven compounds. Antibacterial activity of crude and pure compounds displayed a strong inhibitory activity against Bacillus subtilis. C1, C4, C5 showed moderate activity against Escherichia coli, followed by C2, C3. Meanwhile,\ Staphylococcus aureus was less susceptible to pure compounds with low activity of C3, C5. The crude extract and pure compounds displayed biofilm inhibition activity against four pathogenic bacterial strains. Conclusion: Shrimp shell wastes are one of the economic bioproducts used for the production of bioactive secondary metabolites from fungi.

}, keywords = {Antibacterial activity, Antibiofilm activity, GC/MS analysis, Sarocladium kiliense SDA20, Secondary metabolities, Shrimp waste shell}, doi = {10.5530/pj.2020.12.95 }, author = {Dina M Eskander and Sherien MM Atalla and Ahmed A Hamed and Ezzel -Din A El-Khrisy} } @article {915, title = {Investigation of Secondary Metabolites and Cytotoxicity of Jacquemontia pentantha (Jacq.)}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {July 2019}, pages = {718-723}, type = {Original Article}, chapter = {718}, abstract = {

Introduction: The aim of this study is to isolate and identify sterols and terpenes from the chloroform/methanol extract (3:1) of aerial parts of Jacquemontia pentantha (Jacq.) and evaluation of cytotoxic activity of crude extract and phytol for the first time from this plant. Methods: Different chromatographic techniques for the aerial parts of Jacquemontia pentantha extract were used resulting in isolation of eight compounds. Their structures were elucidated by spectroscopic methods including 1HNMR, 13CNMR, EI/MS spectrometry and by comparing their data with those reported in the literature. The cytotoxicity was evaluated using MTT assay. The mode of action of the extract was predicted by using Enzyme-linked Immunosorbent Assay Kit for Tubulin beta (TUBb). Results: Eight compounds for the first time from this plant were identified as Palmitic acid (1), Phytol (major) (2), Stigmast-4-en- 3-one (3), mixture of α-amyrin (4) and β{\textendash}amyrin (5), 1,6,10,14,18,22-Tetracosahexaen-3- ol,2,6,10,15,19,23-hexamethyl (all-E) (6) and mixture of α{\textendash} amyrin acetate (7) and β-amyrin acetate (8). The extract showed potent cytotoxic activity on MCF-7 breast carcinoma cell line as well as HCT-116 colon carcinoma cell line at different concentrations (100-6.25 ug/ml) with IC50 (21.8 {\textpm} 0.9) and (40.9 {\textpm} 1.3) respectively. Phytol showed potent cytotoxic activity on MCF-7 cell line at different concentrations (100-12.5 ug/ml) with IC50 (60 {\textpm} 2.4), while it had no cytotoxic effect on HCT-116 cell line. The extract showed significant TUBb polymerization inhibition activity. Conclusion: The extract of aerial parts of Jacquemontia pentantha (Jacq.) and also phytol compound has cytotoxic activity due to the presence of phytochemicals such as sterols and terpenes.

}, keywords = {Cytotoxic activity, Enzyme-Linked Immunosorbent Assay, Jacquemontia pentantha, MTT Assay, Sterols, Terpenes}, doi = {10.5530/pj.2019.11.114}, author = {Dina M Eskander and Ezzel -Din A El-Khrisy and Mary H Grace and Marian Nabil and Mahmoud I Nassar and Marwa M Mounier} }