@article {32, title = {Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {01/2015}, pages = {32-36}, type = {Original Article}, chapter = {32}, abstract = {

Introduction: Plectranthus amboinicus (Lour) Spreng is a known medicinal plant used in Siddha and Ayurveda medicines in India. It has enormous medicinal potential to treat various diseases. Methods: The present study focused on the use of essential oil obtained from the leaves of Plectranthusamboinicusto test cytotoxicity against breast (MCF-7) and colorectal (HT-29) cancer cell lines, to protect DNA from H2O2 induced genotoxicity through comet assay and to treat inflammation in lipopolysaccharide (LPS) induced over expression of matrix metalloproteinase-9 (MMP-9) in human peripheral blood mononuclear cells (PBMCs) by gelatin zymogram and inhibition at transcriptional level confirmed using RT-PCR (reverse transcriptase polymerase chain reaction). Results: Cytotoxicity of essential oil against MCF-7 and HT-29 cancer cell lines revealed the IC50 values of 53 \± 0.01 and 87 \± 0.01 \μg/mL respectively. At 200 \μg/mL essential oil protected against 75\% of DNA damage in 3T3-L1 fibroblast cells. Essential oil showed significant reduction in production of MMP-9 in a concentration dependent manner. Conclusion: Overall, the results showed that essential oil of P. amboinicus is a potent bioactive substance and it could be used in herbal medicine preparations.

}, keywords = {Antigenotoxicity, Cytotoxicity, Essential oil, RT- PCR., Zymogram}, doi = {10.5530/pj.2015.7.3}, author = {Ramaraj Thirugnanasampandan and Gunasekar Ramya and Madhusudhanan Gogulramnath and Rajarajeswaran Jayakumar and M.S. Kanthimathi} } @article {1458, title = {Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil.}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {27th Nov, 2014}, pages = {32-36}, type = {Original Article}, chapter = {32}, abstract = {

Introduction:Plectranthus amboinicus (Lour) Spreng is a known medicinal plant used in Siddha and Ayurveda medicines in India. It has enormous medicinal potential to treat various diseases. Methods: The present study focused on the use of essential oil obtained from the leaves of Plectranthus amboinicus to test cytotoxicity against breast (MCF-7) and colorectal (HT-29) cancer cell lines, to protect DNA from H2O2 induced genotoxicity through comet assay and to treat inflammation in lipopolysaccharide (LPS) induced over expression of matrix metalloproteinase-9 (MMP-9) in human peripheral blood mononuclear cells (PBMCs) by gelatin zymogram and inhibition at transcriptional level confirmed using RT-PCR (reverse transcriptase polymerase chain reaction). Results: Cytotoxicity of essential oil against MCF-7 and HT-29 cancer cell lines revealed the IC50 values of 53 \± 0.01 and 87 \± 0.01\μg/mL respectively. At 200 \μg/mL essential oil protected against 75\% of DNA damage in 3T3-L1 fibroblast cells. Essential oil showed significant reduction in production of MMP-9 in a concentration dependent manner. Conclusion: Overall, the results showed that essential oil of P. amboinicus is a potent bioactive substance and it could be used in herbal medicine preparations.
Key words: Essential oil , Cytotoxicity , Antigenotoxicity, Zymogram, RT- PCR.

}, keywords = {Antigenotoxicity, Cytotoxicity, Essential oil, RT- PCR, Zymogram}, author = {Ramaraj Thirugnanasampandan and Gunasekar Ramya and Madhusudhanan Gogulramnath and Rajarajeswaran Jayakumar and M.S. Kanthimathi} }