@article {2297, title = {Phytochemistry, Antihyperglycemic, Antioxidant and Anti- Inflammatory Properties of Uvaria Chamae and Sida Linifolia Extracts: Potential Implication in Diabetic Disease}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {June 2024}, pages = {582-590}, type = {Original Article}, chapter = {582-590}, abstract = {

Introduction: Uvaria chamae and Sida linifolia are plants traditionally used in Togo in diabetes treatment, an affection that often leads to several complications. This study aimed to evaluate the antihyperglycemic, anti-inflammatory, antioxidant activity and toxicity of these two plants extracts. Methods: A phytochemical analysis was carried out on extracts obtained either by decoction or maceration in ethanol of Uvaria chamae leaves and Sida linifolia whole plant. Evaluation of the antihyperglycemic activity consisted in glucose absorption test using yeast and rats{\textquoteright} muscle and jejunum. DPPH test, total antioxidant capacity assay, hemolysis and egg albumin denaturation inhibition assays and evaluation of extracts acute toxicity were performed. Results: Hydroalcoholic extract of Uvaria chamae showed the strongest antihyperglycemic activity (p\<0.05); the highest phenolic contents (147.93 {\textpm} 1.01 mg/g), the best total antioxidant capacity (153.33 {\textpm} 4.07), the lowest IC50 (μg/mL) for DPPH test (296.96 {\textpm} 91.69), a capacity of hemolysis (825.99 {\textpm} 29.24) and egg albumin denaturation (738.10 {\textpm} 92.26) inhibition assays. In the same way, hydroalcoholic extract of Sida linifolia, showed the strongest antihyperglycemic activity (p\<0.05), the highest phenolic contents (71.60 {\textpm} 2.16 mg/g), the best total antioxidant capacity (146.98 {\textpm} 2.81), lowest IC50 (μg/ mL) for DPPH test (788.28 {\textpm} 112.54), the hemolysis (882.03 {\textpm} 20.86) and egg albumin denaturation (1966.18 {\textpm} 35.94) inhibition assays. None of the extracts showed acute toxicity in rats. Conclusion: the hydroalcoholic leaves extract of Uvaria chamae and of the whole plant of Sida linifolia could be candidates in the treatment of diabetes and its complications.

}, keywords = {Decoction, hyperglycemia, Neuroprotection, Phytochemistry}, doi = {10.5530/pj.2024.16.91}, author = {Sabrina Chris Janiba Sanvee and Bignoate Kombate and Yendub{\'e} Toughelighan Kantati and Pascaline Kindji Kpoyizoun and Essotolom Badjabaissi and Mindede Assih1 Aboudoulatif Diallo and Batomayena Bakoma} } @article {1021, title = {Pharmacognostic Studies and Artemisinin Content of Artemisia Annua L. Grown in Togo}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2019}, pages = {1331-1335}, type = {Original Article}, chapter = {1331}, abstract = {

Objective: Artemisia annua grown in Togo is used as an antimalaria drug. The present study shows a detailed analysis of pharmacognostic evaluation of leaf powder and root that will be used for the purpose of identification, authentication, and consequent standardization. Materials and Methods: Both the leaf and root were evaluated for their macroscopic and microscopic features. The physicochemical parameters of the leaf powder and its phytochemical screening were done based on its total phenols and flavono{\"\i}d content. Artemisinin content was also performed using weigh method after extraction. Results: Physicochemical evaluation yielded water, alcohol, acetone, methanol, chloroform, and petroleum ether soluble extractive values which are 2.25\%, 1.25\%, 4.22\%, 8.12\% and 3.77\% (w/w), respectively. Fluorescence analysis imparted characteristic colors to the leaf powder when observed under visible, UV light 254 and 365 nm. Phytochemical screening of leaf powder showed the presence of alkalo{\"\i}ds, flavono{\"\i}d, and anthracene derivatives. Total phenols and flavono{\"\i}d content were 32.5 {\textpm} 0.67 mEq Gallic Acid/100 mg and 11.3 {\textpm} 1.52. mgEq Quercetin/100 mg, respectively. Artemisinin content value was 0.009\% (w/w). Conclusion: Various pharmacognostic parameters which were evaluated assisted in identification and standardization of A. annua leaf in powder and crude form.

}, keywords = {Artemisia, Artemisinin, Flavonoid, Pharmacognostic, Total Phenols}, doi = {10.5530/pj.2019.11.205}, author = {Messan Koffi Adjogbl{\'e} and Batomayena Bakoma and Kossi Metowogo and Kodjovi Dots{\`e} Amouzou and Yao Potchoo and Kwashie Eklu-gadegbeku and Kodjo A Aklikokou and Menssanvi Gbeassor} } @article {791, title = {Phytochemical Study and Biological Activities of Hydro-Alcoholic Extract of the Leaves of Bridelia ferruginea Benth and its Fractions}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {January 2019}, pages = {141-145}, type = {Original Article}, chapter = {141}, abstract = {

Context and purpose: The roots of B. ferruginea are used in traditional Togo medicine in the treatment of diabetes. Studies have shown that leaves have a low antihyperglycemic effect. This study aims to improve the effect of leaves. Methods: The hydroalcoholic extract of the leaves underwent a fractionation (separation in cold alcohol) and an OGTT (Oral Glucose tolerance test) on healthy mice, a phytochemical screening and antioxidant tests (in vitro: DPPH and ex vivo: AAPH) were performed on the extract and its fractions. Results: Phytochemical screening of the extract showed the presence of phenols, flavonoids and condensed tannins as well as the absence of condensed tannins, alkaloids, saponosides and anthracene derivatives. The extract also showed antioxidant activity. The supernatant fraction showed antihyperglycemic activity at 100 mg / kg in the 30th and 60th after glucose administration. Phytochemical screening of the supernatant revealed the presence of phenols, flavonoids and condensed tannins. The supernatant also showed more pronounced antioxidant activity than the total extract. Conclusion: In view of these results, the most active fraction obtained by the separation technique in cold alcohol is the supernatant.

}, keywords = {Antioxidant, Bridelia ferruginea, Diabetes, Fractionation, OGTT}, doi = {10.5530/pj.2019.1.23}, author = {Batomayena Bakoma and Sabrina Sanvee and Kossi Metowogo and Yao Potchoo and Kwashie Eklu gadegbeku and Kodjo Aklikokou and Menssanvi Gbeassor} }