@article {689, title = {In vitro Assay of Alpha-Glucosidase Inhibitor Activities of Three Seagrasses from Banten Bay, Indonesia}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {907-910}, type = {Original Article}, chapter = {907}, abstract = {

Introduction: Diabetes mellitus (DM) is an endocrine disorder characterized by high blood glucose levels. One of the approaches for treating DM is by regulating the postprandial blood glucose via inhibition of \α-glucosidase enzymes. Seagrass is known as a plant containing bioactive compounds, especially for therapy antidiabetic. This research aims to evaluate the \α-glucosidase inhibition activity of three species seagrasses (Enhalus acoroides, Thalassia hemprichii and Cymodocea rotundata) from the Banten Bay, Indonesia. Methods: The extracts of all parts (root, leaf, and stem) from three seagrasses were prepared with maceration method using 80\% ethanol solvents. The \α-glucosidase inhibitory activity was conducted by microplate reader at 400 nm using acarbose as a positive control. Furthermore, the kinetics of \α-glucosidase inhibition, phytochemical screening, and total phenolics content were evaluated against extract which has the most potential \α-glucosidase inhibition. Results: The result showed that the 80\% ethanol extracts of Enhalus acoroides (IC50 values 168.15 \± 2.71 g/mL) had the most potential \α-glucosidase inhibitors activity compared with the positive control acarbose, Thalassia hemprichii and Cymodocea rotundata (IC50 values 197.27 \± 3.07 g/mL, 425.86 \± 5.15 g/mL and 429.28 \± 8.89 g/mL). The kinetic type of inhibition against \α-glucosidase was noncompetitive inhibition. The phytochemical compounds were phenols, flavonoid, terpenes, and tannin with the total phenolic content was 28.76 \± 2.46 mgGAE/g. Conclusion: Enhalus acoroides has the strongest inhibitor of \α-glucosidase and can be further developed for DM therapy agents.

}, keywords = {Cymodocea rotundata, Diabetes mellitus, Enhalus acoroides, Phytochemical compound, Thalassia hemprichii}, doi = {10.5530/pj.2018.5.152}, author = {Agung Widiyanto and Effionora Anwar and Tati Nurhayati} }