@article {62, title = {GC-MS Analysis of Phytocomponents in, Pet Ether Fraction of Wrightia tinctoria Seed}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {Jul-Aug 2015}, pages = {249-253}, type = {Original Article}, chapter = {249}, abstract = {

Introduction: Wrightia tinctoria R.Br. (Family: Apocynaceae) commonly called \“Indrajau\” is well known in Indian traditional system for its traditional uses. Materials and Methods: The present investigation was carried out to determine the possible bioactive components of plant seed ethanolic extract, pet ether fraction using GC-MS analysis. 22 components were identified from pet ether fraction obtained from elution of ethanolic extract packed in silica column. Results: The prevailing compounds from fraction F6to F9 were [1,1\&$\#$39;-Bicyclopropyl]-2-octanoic acid, 2\&$\#$39;-hexyl-, methyl ester (21.39\%) , Trilinolein (7.74\%), 2-Myristynoyl pantetheine (18.07\%), 9-Octadecen-12-ynoic acid, methyl ester (4.46\%), 1Hexadecanol,2-methyl (3.77\%), Cyclopropane tetradecanoic acid, 2-octyl-, methyl ester (2.36\%), 1b, 4a-Epoxy-2H-cyclopenta [3,4] cyclopropa [8,9]cycloundec [1,2-b]oxiren-5 (6H)-one, 7-(acetyloxy) decahydro-2,9,10-trihydroxy-3,6,8,8,10a-pentamethyl (38.91\%), Geranyl isovalerate (23.58\%), cis-13-Octadecenoic acid (5.91\%), Quassin (3.82\%), cis-10-Heptadecenoic acid (3.08\%), 9,12,15-Octadecatrienoic acid 2-phenyl-1, 3-dioxan-5-yl ester (31.50\%), 9,12,15-Octadecatrienoic acid, (Z,Z,Z)-2,3-dihydroxypropyl ester (14.35\%), Cyclopropanebutanoic acid, 2-[ [2-[ [2- [(2-pentylcyclopropyl) methyl] cyclopropyl] methyl] cyclopropyl] methyl]-, methyl ester (10.13\%), 6,9,12,15-Docosatetraenoic acid, methyl ester (3.39\%), 9,12-Octadecadienoic acid, (2-phenyl-1,3-dioxolan-4-yl) methyl ester, trans-( 2.73\%), 9,12-Octadecadienoic acid, (2-phenyl-1,3-dioxolan-4-yl) methyl ester, cis-(4.34\%), Ursodeoxycholic acid (7.14\%), Bufa-20,22-dienolide, 3-(acetyloxy)-14,15-epoxy-16-hydroxy-, (3\á,5\á,15\á,16\á)-(4.75\%), 5H-Cyclopropa [3,4] benz [1,2-e]azulen-5-one, 9a (acetyloxy)-1,1a,1b,4,4a,7a,7b,8,9,9a-de cahydro-4a,7b,9-trihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-,[1aR-(1a\à,1b\á,4a\á,7a\à,7b\à,8\à,9\á,9a\à)]-(6.59\%), Docosahexaenoic acid, 1,2,3-propanetriyl ester (10.86\%), Olean-12-ene-3,15,16,21,22,28-hexol, (3\á,15\à,16\à,21\á,22\à)-( 4.40\%) found as the major components. Conclusion: It could be concluded that, Wrightia tinctoria contains various bioactive compounds. So it is recommended as a plant of phytopharmaceutical importance

}, keywords = {Bioactive components, Ethanolic extract, GC-MS, Indrajau, Wrightia tinctoria}, doi = {10.5530/pj.2015.4.7}, author = {Rajani Srivastava and Amita Verma}, editor = {Alok Mukerjee} } @article {1452, title = {GC-MS Analysis of Phytocomponents in, Pet Ether Fraction of Wrightia tinctoria Seed.}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {29th Apr, 2015}, pages = {249-253}, type = {Original Article}, chapter = {249}, abstract = {

Introduction:Wrightia tinctoria R.Br. (Family: Apocynaceae) commonly called \“Indrajau\” is well known in Indian traditional system for its traditional uses. Materials and Methods: The present investigation was carried out to determine the possible bioactive components of plant seed ethanolic extract, pet ether fraction using GC-MS analysis. 22 components were identified from pet ether fraction obtained from elution of ethanolic extract packed in silica column. Results: The prevailing compounds from fraction F6to F9 were [1,1\&$\#$39;-Bicyclopropyl]-2-octanoic acid, 2\&$\#$39;-hexyl-, methyl ester (21.39\%) , Trilinolein (7.74\%), 2-Myristynoyl pantetheine (18.07\%), 9-Octadecen-12-ynoic acid, methyl ester (4.46\%), 1Hexadecanol,2-methyl (3.77\%), Cyclopropane tetradecanoic acid, 2-octyl-, methyl ester (2.36\%), 1b, 4a-Epoxy-2H-cyclopenta [3,4] cyclopropa [8,9]cycloundec [1,2-b]oxiren-5 (6H)-one, 7-(acetyloxy) decahydro-2,9,10-trihydroxy-3,6,8,8,10a-pentamethyl (38.91\%), Geranyl isovalerate (23.58\%), cis-13-Octadecenoic acid (5.91\%), Quassin (3.82\%), cis-10-Heptadecenoic acid (3.08\%), 9,12,15-Octadecatrienoic acid 2-phenyl-1, 3-dioxan-5-yl ester (31.50\%), 9,12,15-Octadecatrienoic acid, (Z,Z,Z)-2,3-dihydroxypropyl ester (14.35\%), Cyclopropanebutanoic acid, 2-[ [2-[ [2- [(2-pentylcyclopropyl) methyl] cyclopropyl] methyl] cyclopropyl] methyl]-, methyl ester (10.13\%), 6,9,12,15-Docosatetraenoic acid, methyl ester (3.39\%), 9,12-Octadecadienoic acid, (2-phenyl-1,3-dioxolan-4-yl) methyl ester, trans-( 2.73\%), 9,12-Octadecadienoic acid, (2-phenyl-1,3-dioxolan-4-yl) methyl ester, cis-(4.34\%), Ursodeoxycholic acid (7.14\%), Bufa-20,22-dienolide, 3-(acetyloxy)-14,15-epoxy-16-hydroxy-, (3\á,5\á,15\á,16\á)-(4.75\%), 5H-Cyclopropa [3,4] benz [1,2-e]azulen-5-one, 9a (acetyloxy)-1,1a,1b,4,4a,7a,7b,8,9,9a-de cahydro-4a,7b,9-trihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-,[1aR-(1a\à,1b\á,4a\á,7a\à,7b\à,8\à,9\á,9a\à)]-(6.59\%), Docosahexaenoic acid, 1,2,3-propanetriyl ester (10.86\%), Olean-12-ene-3,15,16,21,22,28-hexol, (3\á,15\à,16\à,21\á,22\à)-( 4.40\%) found as the major components. Conclusion: It could be concluded that, Wrightia tinctoria contains various bioactive compounds. So it is recommended as a plant of phytopharmaceutical importance.

Key words: Bioactive components, Ethanolic extract, GC-MS, Indrajau, Wrightia tinctoria.

}, keywords = {Bioactive components, Ethanolic extract, GC-MS, Indrajau, Wrightia tinctoria.}, author = {Rajani Srivastava and Alok Mukerjee and Amita Verma} } @article {1503, title = {High-Performance Thin-Layer Chromatography Fingerprinting of Ethnopharmacological Important Seeds of Wrightia tinctoria}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {3rd Sept, 2014}, pages = {10-14}, type = {Original Article}, chapter = {10}, abstract = {

Introduction:Wrightia tinctoria R. Br. belongs to family Apocynaceae commonly called as sweet Indrajao, Pala indigo plant, Dyer\’s Oleander. \“Jaundice curative tree\” in south India. Materials and Methods: A method has been developed for different extracts of W. tinctoria for high-performance thin-layer chromatography (HPTLC) fingerprinting analysis for identification and quantification of the marker compound. Result: The satisfactory resolution was obtained in the solvent system toluene:ethyl acetate v/v (8:2) for petroleum ether extract, toluene:ethyl acetate v/v (7:3) for chloroform extract and toluene:ethyl acetate:formic acid v/v (7:3:0.1) for alcoholic extract. Conclusion: The HPTLC fingerprinting profile developed for different extracts of W. tinctoria will help in proper identification and quantification of the marker compound.

Key words: High-performance thin-layer chromatography fingerprinting, Wrightia tinctoria, petether extract, chloroform extract, alcoholic extract.

}, keywords = {Alcoholic extract, chloroform extract, High-performance thin-layer chromatography fingerprinting, petether extract, Wrightia tinctoria}, author = {Rajani Srivastava and Alok Mukerjee and Amita Verma} }