@article {713, title = {Immunostimulatory Activity of Chitosan Nanoparticles on Wistar Albino Rats}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {892-898}, type = {Original Article}, chapter = {892}, abstract = {

Background: The active components of natural products provide a potential alternative to conventional immunotherapy for a variety of diseases conditions and become subject to scientific investigations currently worldwide. Objective: The purpose of this research was to investigate the immunostimulatory activity of the chitosan nanoparticle on Wistar albino rats. Materials and Methods: The present investigation was carried out on various groups of healthy adult rats. The assessment of immunomodulatory potential was carried out by neutrophil adhesion test, delayed-type hypersensitivity (DTH) response, haemagglutinating antibody (HA) titre, cyclophosphamide-induced myelosuppression and phagocytic activity were determined in various groups of animals. Results: The administration of chitosan nanoparticle at doses 300 mg/kg BW and 600 mg/kg BW but not at doses 150 mg/kg BW significantly increased in neutrophil adhesion fibers, haemagglutinating antibody titre values and potentiated the inhibited type hypersensitivity reaction induced by sheep red blood cells. Also, it had good response towards phagocytosis in carbon clearance assay and prevented myelosuppression of cyclophosphamide on rats. Conclusion: From these findings, it can be concluded that chitosan nanoparticle responsible for immunostimulatory activity and has therapeutic potential for the prevention of immune depressed conditions.

}, keywords = {Chitosan nanoparticle, Delayed-Type Hypersensitivity, Haemagglutinating antibody, Myelosuppression, Neutrophil adhesion, Phagocytosis}, doi = {10.5530/pj.2018.5.150}, author = {Giftania Wardani and Mahmiah and Sri Agus Sudjarwo} } @article {488, title = {In vitro Antibacterial Activity of Chitosan Nanoparticles against Mycobacterium tuberculosis}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {December 2017}, pages = {162-166}, type = {Original Article}, chapter = {162}, abstract = {

Background: Chitosan nanoparticles have gained growing interest for nanomedicine, biomedical engineering and development of new therapeutic with improved bioavailability, increased sensitivity and specificity, and reduced toxicity. Objective: The aim of the present study is to synthesis of the chitosan nanoparticles for antimycobacterial applications. Methods: Chitosan were isolated from the shrimp shell. Tripolyphosphate (TPP) will be used to prepare chitosan nanoparticles by ionotropic gelation method. The size and morphology of the chitosan nanoparticle was analyzed by scanning electron microscope (SEM). The broth microdilution method is used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of nanoparticle chitosan on strain M. tuberculosis H37Rv. Results: The SEM micrographs of the nano-magnetic chitosan showed that they were approximately uniform spheres and the rough surface morphology, have a solid dense cubical or rectangular structure. Using the broth microdilution susceptibility method, chitosan nanoparticle was found to have the antimycobacterial effects with a MIC value of 1200 \μg/mL whilst MBCs value of 2400 \μg/mL for M. tuberculosis H37Rv. Conclusion: The conclusion from the study was chitosan nanoparticle have potential as a source of lead compounds that may be developed further into antimycobacterial drugs.

}, keywords = {Chitosan nanoparticle, M. tuberculosis H37Rv, Minimal bactericidal concentration., Minimal inhibitory Concentration}, doi = {10.5530/pj.2018.1.27}, url = {http://fulltxt.org/article/413}, author = {Giftania Wardani and Mahmiah and Sri Agus Sudjarwo} }