@article {1075, title = {Comparative Pharmacognostical and Pharmacological Evaluation of Costus speciosus (Koen) J.E. Sm. Germplasm Collected from Eastern Ghats of India}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {February 2020}, pages = {150-156}, type = {Research Article}, chapter = {150}, abstract = {

Background: Costus speciosus is an erect perennial herb belonging to family Costaceae, an important medicinal plant widely used in several indigenous medicinal formulations. Objective: A comparative evaluation of Pharmacognostical and Pharmacological potential of Costus speciosus for the validation of traditional claims and quality parameters for industry. Materials and Methods: Pharmacognostical studies were performed as per Ayurvedic Pharmacopeia of India and quantification of diosgenin was done through HPTLC. In vitro antidiabetic activity was evaluated by α-amylase inhibition assay based on starch iodine method and in vitro anti-inflammatory were done by using inhibition of protein denaturation assay. Results: The pharmacognostical standards were also laid down for each sample. Morpho-anatomical characters had no distinct variation in all the collected samples of Eastern Ghats. The quantification of diosgenin (without hydrolysis of samples) in the collected germplasm varies significantly from 0.002 to 0.076 \% on dry weight basis. The maximum content was recorded in NBCS-06 from Patiya, Bhubaneswar and was identified as distinct chemotype with high metabolite content. IC50 value of Costus speciosus extract in starch-iodine assay was found to be maximum in NBCS- 6 (87.54 μg/ml) and inhibition of protein denaturation assay was found to be maximum in NBCS- 11 (73.91 μg/ml), respectively. Conclusion: The study suggests that the Costus speciosus germplasm possess potential anti-inflammatory and anti-diabetic activity and comparative pharmacognostical parameters will be useful in collection of location specific potential samples for industrial usage along with quality control of raw materials.

}, keywords = {Anti-diabetic, Anti-inflammatory, Costus speciosus, Diosgenin, HPTLC}, doi = {10.5530/pj.2020.12.22}, author = {Manish Kumar and Ankita Misra and Akanksha Srivastava and Pushpendra Kumar Shukla and L M Tewari and Sharad Srivastava} } @article {605, title = {Pharmacognostic and Pharmacological Evaluation of Hyssopus officinalis L. (Lamiaceae) Collected from Kashmir Himalayas, India}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {June 2018}, pages = {690-693}, type = {Original Article}, chapter = {690}, abstract = {

Introduction: Hyssopus officinalis L. is a well-known herb for its culinary and medicinal significance. The purpose of this study was to perform the pharmacognostic evaluation. Methods: Physicochemical and phytochemical analysis, HPTLC quantification and in vitro antioxidant and antidiabetic activity were done. Results: Preliminary screening revealed the presence of phytomolecules such as alkaloid (0.99\%), tannin (1.75\%), sugar (1.96\%) and starch (0.68\%). Total phenolic and flavonoid content were found to be 2.32\% and 1.16\% respectively. HPTLC quantification data showed that the content of ferulic acid (0.034\%) was higher than caffeic acid (0.0064\%) on dry weight basis The IC50 value for the in vitro DPPH radical scavenging assay was 0.50 \μg/ml and in vitro anti diabetic assay displayed IC50 value of 0.8366 mg/ml. Conclusion: The study suggests presence of considerable amount of phenolic acids and antioxidant activity in the plant which supports its use in the traditional systems of medicine.

}, keywords = {Antioxidant, DPPH, HPTLC, Hyssopus officinalis, Phenolic acids}, doi = {10.5530/pj.2018.4.114}, url = {http://fulltxt.org/article/652}, author = {Akanksha Srivastava and Kuldeep Awasthi and Bhanu Kumar and Ankita Misra and Sharad Srivastava} } @article {450, title = {Evaluation of Anti Arthritic Potential of Gloriosa superba (L.) Elite Germplasm Collected from Eastern Himalayas, India}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {November 2017}, pages = {s87-s92}, type = {Original Article}, chapter = {s87}, abstract = {

Introduction: Gloriosa superba (L.) is a traditionally known medicinal plant for its potential antigout property. The species is rich source of colchicine alkaloid and is commercially exploit in the international market for the same. Method: In the present study, elite chemotype of G. superba was identified from natural population in Eastern Himalayas based on their colchicine content through HPTLC calibrated method. The selected elite chemotypes were further evaluated for in vitro anti-arthritic potential via inhibition of protein denaturation along with hydroxyl radical scavenging potential. Result: The HPTLC quantification data reveals that the content of colchicine varies from 0.044 to 0.184\% having maximum content in NBG-128 from Jorhat, Assam. The results of bioassay reflect a potentiating anti-arthritic and hydroxyl radical scavenging with statistically insignificant difference within the elite germplasms. Conclusion: The presence of bioactive polyphenolics with significant hydroxyl radical scavenging will further suggest that inhibition of inflammatory mediator cells by extract is superimposed action of colchicine and other chemical inhibitors like polyphenolics. The study will aid in site specific exploration of high metabolite yielding chemotype(s) with validated pharmacological action for commercial cultivation to meet out the industrial demand of colchicine and herbal product development.

}, keywords = {Anti arthritic, Colchicine, Elite chemotype, G. superba, HPTLC}, doi = {10.5530/pj.2017.6s.162}, url = {http://fulltxt.org/article/387}, author = {Ankita Misra and Akanksha Srivastava and Mohammad Khalid and Poonam Kushwaha and Sharad Srivastava} }