@article {622, title = {Hydroalcoholic and Alkaloidal Extracts of Murraya koenigii(L.) Spreng Augments Glucose Uptake Potential against Insulin Resistance Condition in L6 Myotubes and Inhibits Adipogenesis in 3T3L1 Adipocytes}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {June 2018}, pages = {633-639}, type = {Original Article}, chapter = {633}, abstract = {

Background: Murraya koenigii, commonly known as \“curry leaves\” is native to India. The highly valued part of the plant is the leaves which possess various biological activities. Objective: The present study aimed to investigate the antidiabetic effect of Murraya koenigii (MK) leaf extracts, of two different solvent ratios. Materials and methods: 70\% hydroalcoholic and alkaloidal extracts of MK leaves were prepared by cold maceration method. Preliminary phytochemical analysis was carried out for both the extracts. In vitro anti diabetic activity was screened by inhibitory action on \α \– amylase, \α \– glucosidase enzymes. Further, the 70\% hydroalcoholic and alkaloidal extracts were assessed for glucose uptake potential, anti - adipogenic property, as well as inhibitory activity on diabetes associated complications. HPTLC quantification of major phytoconstituent was carried out. Results: The study showed presence of various phytoconstituents such as, polyphenols, alkaloids, tannins, reducing sugars etc. The 70\% hydroalcoholic and alkaloidal extracts of MK leaves exhibited \>90\% inhibition against carbohydrate metabolising enzymes compared to aqueous and absolute alcohol extracts. Both the extracts showed enhanced glucose uptake in L6 myotubes attenuating the effect of Palmitate induced insulin resistance. Significant inhibition on adipogenesis was exerted by both 70\% hydroalcoholic and alkaloidal extracts of MK leaves. Besides, marked inhibition of advanced glycation end products was exhibited by the extracts. HPTLC quantification analysis of the aforementioned extracts showed the presence of major phytoconstituent, Mahanine, in it. Conclusion: The results of the present study showed that MK possesses significant antidiabetic property and also exhibited considerable effect in preventing diabetes associated complications. The potent antidiabetic activity of MK could be attributed to the presence of Mahanine, the major active constituent, which is a carbazole alkaloid.

}, keywords = {complications, Diabetes, glucose uptake, L6 myotubes, Mahanine, α - amylase}, doi = {10.5530/pj.2018.4.104}, url = {http://fulltxt.org/article/642}, author = {Parameswari Royapuram Parthasarathy and Janani Murthy and Dinesh Murugan Girija and Srivani Telapolu and Chamundeeswari Duraipandian and Thyagarajan Sadras Panchatcharam} } @article {382, title = {Cytotoxic and Antioxidant Effects in Various Tissue Extracts of Plumbago zeylanica: Implications for Anticancer Potential}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {July 2017}, pages = {706-712}, type = {Original Article}, chapter = {706}, abstract = {

Introduction: Complex natural products are emerging as a potent alternate and complement in anticancer therapies due to its multiple mechanisms of action and minimal effects on nontarget cells/tissues. Some of the traditional herbal formulations contain Plumbago zeylanica L, (commonly known as Chitraka or Chitramoolam) to treat various disorders for centuries in Africa and Asia. Methods: The quantity of Plumbagin was detected in different tissues of P. zeylanica through HPTLC analysis. We evaluated and compared the alcoholic tissue extracts of leaf, stem and root of P. zeylanica with standard pure Plumbagin for its in vitro cytotoxic effects (metabolic viability; MTT assay) in A549 Lung cancer cells and antioxidant potential (DPPH free radical scavenging and total phenol content). RNA sequencing and transcriptome analysis was performed in the most potent tissues of P. zeylanica demonstrating efficient pharmacological activity. Results: The root extract containing 1.3\% of plumbagin exhibited strongest scavenging potential with 50\% radical scavenging (IC50\ value of 3.99 g) in comparison with 35.85 g in stem (0.2\% Plumbagin) and 18.06 g in leaf with 0.00007\% Plumbagin. The in vitro cytotoxicity in A549 cells also showed that the root extract was most potent with an IC50\ value of 164.5\ g as compared to extracts from stem (IC50= 379.5\μg) and leaf (IC50= 274.9 g). Conclusion: The strong antioxidant potential shown by the P. zeylanica extract demonstrates its ability to protect the non-target (normal) cells against oxidative stress, while the cytotoxic effects suggest that it could inhibit the growth of cancerous cells. Further, transcriptome analysis predicted the expression of potent gene transcripts coding for quinone biosynthesis in the root tissues of P. zeylanica.

}, keywords = {A549 Cell Line, Alcoholic Root Extract, DPPH assay, HPTLC Quantification, Plumbagin, Transcriptome Analysis}, doi = {10.5530/pj.2017.5.111}, url = {/files/pj-9-5/10.5530pj.2017.5.111/index.html}, author = {Balachandran Karpaga Raja Sundari and Srivani Telapolu and Bilikere S and Dwarakanath and Sadras. P. Thyagarajan} }