@article {1408, title = {Preliminary Phytochemical Studies, GC-MS Analysis and In vitro Antioxidant Activity of Selected Medicinal Plants and its Polyherbal Formulation}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {May 2021}, pages = {648-659}, type = {Original Article}, chapter = {648}, abstract = {

Background: Novel polyherbal formulation (PHF) is the utilization of more than one herb in the preparation of herbal medication. The thought is found in the conventional system of medicine where the variety of herbs in a specific proportion of illness. Because of synergism, polyherbalism presents a few advantages which aren{\textquoteright}t accessible in single herbal medication. It is utilized in these medications for the treatment of numerous sicknesses including antioxidants. Objective: To develop a phytochemical screening and GC-MS analysis of Novel Polyherbal formulation for In vitro antioxidant activity. Materials and Methods: Macroscopical, preliminary phytochemical, quantitative phytoconstituents, and In-vitro antioxidant activity of all the individual extract and polyherbal formulation was done by chemical method. Identification of phytoconstituents with the aid of Gas chromatography {\textendash} Mass spectroscopy (GC-MS). Results: Macroscopical study and physicochemical examination, for example, ash value, extractive value, loss on drying, and pH were reported to A. racemosus, B. variegata, C. bonducella, S. asoka, and S. racemosus and novel polyherbal formulation. Qualitative phytochemical investigation revealed the presence of alkaloids, flavonoids, gums \& mucilage, carbohydrates, steroids, proteins \& amino acids, fats \& fixed oils, glycoside, phenols, and saponins. Quantitative estimation such as TAC, TFC, TGC, TSC, and TPC was showed positive results. All the individual extract and PHF were subjected to GC-MS analysis. All the individual extract and polyherbal formulation displayed strong antioxidant activity. Conclusions: To conclude the PHF was reported that high level of bioactive contents present and strong antioxidant activity in contrast to the preferred ascorbic acid. The GC-MS uncovered the presence of bioactive compounds and these compounds are suggested to treat antibacterial, antioxidant, anti-inflammatory, and antiviral, anti-tumor, anti-proliferative activity, and antifungal activity.

}, keywords = {Antioxidant, GC-MS analysis, Macroscopical, Phytochemical, Polyherbal formulation}, doi = {10.5530/pj.2021.13.83}, author = {Shalini K and Ilango K} } @article {1489, title = {Development and validation of a RP-HPLC method for the simultaneous determination of Mangiferin, Ellagic acid and Hydroxycitric acid in polyherbal formulation}, journal = {Pharmacognosy journal}, volume = {6}, year = {2014}, month = {8th April 2014}, pages = {23-28}, type = {Original Article}, abstract = {

The US patented polyherbal formulation for the prevention and management of Type II diabetes and its vascular complications was used for the present study. The formulation consists of roots of Salacia species, leaves of Lagestroemia parviflora and fruit rind of Garcinia indica. The use of reversed phase C18 HPLC column was used and eluted with isocratic mobile phase of acetonitrile and phosphoric acid buffer solution enabled the efficient separation of chemical markers within 20min. Validation of the method was performed in order to demonstrate its selectivity, accuracy, precision, repeatability and recovery. All calibration curve shows good linear correlation coefficients (r2\>0.995) within tested ranges. Three markers in this polyherbal formulation were quantified were Mangiferin (1.53\% w/w), Ellagic acid (0.9655 w/w), Hydroxycitric acid (5.3\% w/w). Intra and inter day RSDs of retention times and peak areas were less than 3\%. The recoveries were between 95\% and 102.5\%. In conclusion a method has been developed for the simultaneous quantification of three markers in this polyherbal formulation. The established RP-HPLC method was simple, precise and accurate and can be used for the quality control of the raw materials as well as formulations.

Key words: Polyherbal formulation, Mangiferin, Ellagic acid, Hydroxycitric acid, RP-HPLC.

}, keywords = {Ellagic acid, Hydroxycitric acid, Mangiferin, Polyherbal formulation, RP-HPLC}, author = {Ananth Kumar Kammalla, and Mohan Kumar Ramasamy, and Agarwal Aruna, and Dubey GP, and Ilango Kaliappan} }