@article {587, title = {Inhibitory effect of Bryophyllum pinnatum (Lam.) Oken leaf extract and their fractions on α-amylase, α-glucosidase and cholinesterase enzyme}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {March 2018}, pages = {497-506}, type = {Original Article}, chapter = {497}, abstract = {

Bryophyllum pinnatum (Lam.) Oken leaves are employed as food and as traditional medicines. This study investigates the antioxidant activity (reducing power, DPPH, ABTS, FRAP, H2O2 scavenging ability and metal ion chelating), carbohydrate digesting enzymes activity and inhibitory activity of cholinergic enzyme of aqueous extract and fractions (n-hexane, ethyl acetate, n-butanol, residual aqueous fraction) of B. pinnatum leaves were investigated. Results showed that aqueous extract of B. pinnatum exhibited DPPH radical scavenging abilities, iron chelation, hydrogen peroxide scavenging abilities and reducing power (Fe3+- Fe2+). B. pinnatum aqueous extract also had considerably \α-amylase and \α-glucosidase inhibitory activities with IC50 values 149.20 \± 14.44 \μg/mL and 126.15 \± 9.76 \μg/mL respectively. Our findings indicated that ethyl acetate fraction contained a considerably higher (p \< 0.05) amount of total phenolic, flavonoids, total antioxidant, FRAP, metal ion, ABTS and DPPH radical scavenging activity than other solvent fractions. Furthermore, the ethyl acetate fraction elicited a significantly higher (p \< 0.05) inhibitory effects on \α-glucosidase (IC50 = 70.90 \± 1.23 \μg/ml), \α-amylase (IC50 = 62.45 \± 1.22 \μg/ml), acetylcholinesterase (AChE) (IC50 = 66.75 \μg/mL) and butyrylcholinesterase (BChE) (IC50 = 62.97 \μg/mL) activities than other fractions. Hence, B. pinnatum leaves were rich in biologically active components; thus, could be employed to formulate new plant-based pharmaceutical and nutraceutical drugs to improve human health.

}, keywords = {Antioxidants, Bryophyllum pinnatum (Lam.), Cholinesterase, Enzyme inhibitory activity, Oken}, doi = {10.5530/pj.2018.3.82}, url = {http://fulltxt.org/article/515}, author = {Oluwafemi Adeleke Ojo and Adebola Busola Ojo and Basiru Olaitan Ajiboye and Oluranti Olaiya and Ayodeji Akawa and Oyindamola Olaoye and Omosola Olufisayo Anifowose and Olajumoke Idowu and Oluwaseun Olasehinde and Tajudeen Obafemi and Joseph Awe and Babatunji Emmanuel Oyinloye} }