@article {2609, title = {Total Phenolic and Flavonoid Contents, Antioxidant and Antiinflammatory Activities of Phellinus rimosus Extracts}, journal = {Pharmacognosy Journal}, volume = {18}, year = {2026}, month = {June 2026}, pages = {181-186}, type = {Original Article}, chapter = {181}, abstract = {

Background: Phellinus rimosus is a medicinal mushroom with potential bioactive properties, although scientific evidence supporting its pharmacological activities remains limited. Objective: To investigate the phenolic and flavonoid contents, antioxidant capacity, and anti-inflammatory activity of ethanolic and aqueous extracts of P. rimosus. Materials and Methods: Total phenolic content (TPC) and total flavonoid content (TFC) were determined using colorimetric assays. Antioxidant activity was evaluated by DPPH, ABTS, and FRAP methods. Anti-inflammatory activity was assessed by measuring nitric oxide (NO) inhibition in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Statistical analysis was performed at P \< 0.05. Results: The ethanolic extract contained significantly higher TPC (361.04 {\textpm} 5.69 mg GAE/g extract) and TFC (646.55 {\textpm} 6.29 mg RE/g extract) than the aqueous extract. It exhibited strong antioxidant activity with IC$_{5}$$_{0}$ values of 9.56 {\textpm} 0.47 μg/mL (DPPH) and 5.40 {\textpm} 0.06 μg/mL (ABTS), and a FRAP value of 52.39 {\textpm} 0.25 mM Fe{\texttwosuperior}$^{+}$/100 mg extract. The ethanolic extract also significantly inhibited NO production (IC$_{5}$$_{0}$ = 54.40 {\textpm} 2.35 μg/mL) without cytotoxicity, whereas the aqueous extract showed no inhibitory effect. Conclusion: The ethanolic extract of P. rimosus demonstrates notable antioxidant and anti-inflammatory activities and may serve as a promising natural source of bioactive compounds.

}, keywords = {Anti-inflammatory activity, antioxidant activity, Flavonoid content, nitric oxide inhibition, Phellinus rimosus, Phenolic content}, doi = {10.5530/pj.2026.18.131}, author = {Pornpun Laovachirasuwan and Sonesay Thammavong and Khwanruan Naksuwankul and Sounantha suvanlasy and Theera Rittirod and Weerapong Prasongchean and Jidapha Sangmahachai and Sutthipong Chiangtong and Panwad Lerdpiriyasakulkit and Burin T. Sriwong and Piyachet Jatuten and Methin Phadungkit} }