@article {2495, title = {Phytochemical Screening, Antioxidant Potential, and α-Glucosidase Inhibition of Causonis trifolia Leaf Extracts: A Solvent-Based Comparative Study}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {April 2025}, pages = {164-170}, type = {Original Article}, chapter = {164}, abstract = {

Introduction: Medicinal plants are used in diabetes mellitus due to their proven minimal adverse effects in humans. Causonis trifolia leaves have been used as traditional medicine for various treatments. Objective: The aim of this research was to investigate the phytochemicals, the total flavonoid and phenolic content, and in-vitro antioxidant and antidiabetic activities of the extracts of C. trifolia leaves. Method: The leaves of C. trifolia were sequentially extracted with maceration in hexane, ethyl acetate, ethanol, and 50\% ethanol. Then, the C. trifolia leaf extracts were assessed for antioxidant activity by the DPPH and ABTS radical scavenging and FRAP assay. Total phenolic and flavonoid contents were determined by the Folin- Ciocalteu and aluminum chloride colorimetric methods, respectively. Antidiabetic activity was evaluated by determination of α-glucosidase inhibitory effect. Results: Preliminary phytochemical analysis of ethanol and 50\% ethanol extracts of C. trifolia leaves showed positive results for alkaloids, coumarin, tannins, saponins, cardiac glycosides, terpenoids, and steroids. The 50\% ethanol extract had the best antioxidant activities of the three antioxidant assays (p \< 0.05). The results revealed higher phenolic and flavonoid contents in 50\% ethanol extract than in the other extracts (p \< 0.05). Moreover, the 50\% ethanol extract exhibited the best potential as α-glucosidase inhibitors of all the extracts and acarbose (p \< 0.05). Conclusions: The results conclude that the C. trifolia leaves with 50\% ethanol as the solvent possessed the potential to extract the highest levels of phytochemical content and have potential antioxidant and α-glucosidase inhibitory activities for diabetic therapy.

}, keywords = {Antidiabetic, Antioxidant, Causonis trifolia, α-glucosidase}, doi = {10.5530/pj.2025.17.20}, author = {Patcharin Singdam and Anussara Kamnate and On-Anong Somsap and Ruhainee Tohkayomatee} }