In Vitro Antioxidant Activity of Extracts from Fruits of Muntingia calabura Linn. from India

Abstract:

Introduction: In the present study, hexane, chloroform, ethyl acetate, butanol and methanol extracts of fruits of
Muntingia calabura L (Elaeocarpaceae) were examined total phenolics (TP) and in vitro antioxidative capacity.
Methods: For the determination of total phenolics (TP) and in vitro antioxidative capacity, established assay methods
such as 1, 1 - diphenyl – 2- picryl hydroxyl (DPPH) radical assay, reducing power, ferric ion chelating assay, superoxide
anion, and nitric oxide scavenging activity assays were used with reference to synthetic antioxidant butyl hydroxyl
toluene (BHT). One way analysis of variance (ANOVA) and Duncan’s Multiple Range Test (DMRT) were carried out.
Results: The TP ranged from 1486 ± 0.028 mg GAE /100 of fresh mass (FW) to 358 ± 0.020 mg GAE/100 of fresh mass
(FW). The data obtained from the study showed high levels of antioxidant activity of the fruit extracts. Conclusion: From
the findings, it was observed that there was a well correlation between antioxidant activity and total phenolic/flavonoid
contents. These results may be useful to further analyze wild edible fruits that contain most antioxidant activity in order
to identify the active constituents.